Abstract:
:It is known that fatty acids (FAs) regulate transcription through a number of FA responsive transcription factors. In order to investigate the effect of FAs on gene regulation in cultured human hepatocytes we examined the effect of palmitate on hepatic glucokinase (GK) promoter activity and expression of transcription factors that regulate GK expression. GK promoter activity was increased in constructs lacking a cAMP response element (CRE), while palmitate incubation decreased GK promoter activity in CRE-negative constructs. Cells exposed to palmitate showed increased levels of PPARalpha apolipoprotein-AII and -B100 mRNA and decreased levels of SREBP-1c mRNA but there was no effect on LXRalpha and HNF-4alpha mRNA. In addition, cDNA microarray analysis of short-term (1.5h) transcriptional regulation by palmitate, oleate, and eicosapentaenoic acid (EPA) identified that oleate and EPA initiated similar changes in the pattern of hepatic gene regulation, whereas palmitate had little effect.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Swagell CD,Henly DC,Morris CPdoi
10.1016/j.bbrc.2007.07.191subject
Has Abstractpub_date
2007-10-19 00:00:00pages
374-80issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(07)01672-5journal_volume
362pub_type
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