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A new comprehensive method for detection of livestock-related pathogenic viruses using a target enrichment system.

Abstract：

:We tested usefulness of a target enrichment system SureSelect, a comprehensive viral nucleic acid detection method, for rapid identification of viral pathogens in feces samples of cattle, pigs and goats. This system enriches nucleic acids of target viruses in clinical/field samples by using a library of biotinylated RNAs with sequences complementary to the target viruses. The enriched nucleic acids are amplified by PCR and subjected to next generation sequencing to identify the target viruses. In many samples, SureSelect target enrichment method increased efficiencies for detection of the viruses listed in the biotinylated RNA library. Furthermore, this method enabled us to determine nearly full-length genome sequence of porcine parainfluenza virus 1 and greatly increased Breadth, a value indicating the ratio of the mapping consensus length in the reference genome, in pig samples. Our data showed usefulness of SureSelect target enrichment system for comprehensive analysis of genomic information of various viruses in field samples.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Oba M,Tsuchiaka S,Omatsu T,Katayama Y,Otomaru K,Hirata T,Aoki H,Murata Y,Makino S,Nagai M,Mizutani T

doi

10.1016/j.bbrc.2017.12.017

subject

Has Abstract

pub_date

2018-01-08 00:00:00

pages

1871-1877

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(17)32402-6

journal_volume

495

pub_type

杂志文章

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