Episodic positive selection during the evolution of naphthalene dioxygenase to nitroarene dioxygenase.

Abstract:

:Using different maximum-likelihood models of adaptive evolution, signatures of natural selective pressure, operating across the naphthalene family of dioxygenases, were examined. A lineage- and branch-site specific combined analysis revealed that purifying selection pressure dominated the evolutionary history of the enzyme family. Specifically, episodic positive Darwinian selection pressure, affecting only a few sites in a subset of lineages, was found to be responsible for the evolution of nitroarene dioxygenases (NArDO) from naphthalene dioxygenase (NDO). Site-specific analysis confirmed the absence of diversifying selection pressure at any particular site. Different sets of positively selected residues, obtained from branch-site specific analysis, were detected for the evolution of each NArDO. They were mainly located around the active site, the catalytic pocket and their adjacent regions, when mapped onto the 3D structure of the α-subunit of NDO. The present analysis enriches the current understanding of adaptive evolution and also broadens the scope for rational alteration of substrate specificity of enzyme by directed evolution.

authors

Dutta A,Chakraborty J,Dutta TK

doi

10.1016/j.bbrc.2013.09.029

subject

Has Abstract

pub_date

2013-10-11 00:00:00

pages

68-75

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(13)01500-3

journal_volume

440

pub_type

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