Abstract:
:Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study, we examined the effects of Hrp3 on heterochromatin silencing using several S. pombe reporter strains. The phenotypic analysis revealed that hrp3(+) is not an essential gene for cell viability. However, Hrp3 is required for transcriptional repression at silence loci of mat3. A chromatin immunoprecipitation assay showed that Hrp3 directly associates with mat3 chromatin. Thus, our results strongly suggest that Hrp3 is involved in heterochromatin silencing and plays a direct role as a chromatin remodeling factor at mat3 in vivo.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Jae Yoo E,Kyu Jang Y,Ae Lee M,Bjerling P,Bum Kim J,Ekwall K,Hyun Seong R,Dai Park Sdoi
10.1016/s0006-291x(02)00797-0subject
Has Abstractpub_date
2002-07-26 00:00:00pages
970-4issue
4eissn
0006-291Xissn
1090-2104pii
S0006291X02007970journal_volume
295pub_type
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