Establishment of a new glial cell line from hippocampus of prion protein gene-deficient mice.


:Cellular prion protein (PrP(C)) is expressed not only in neuronal cells but also in non-neuronal cells such as astroglial cells. In the present study, the prion protein (PrP) gene (Prnp)-deficient astroglial cell line GpL1 from hippocampal cells of ZrchI Prnp(-/-) mice were established. Transfection of Prnp suppressed cell death in GpL1 cells under serum-free conditions. The PrP-expressing GpL1 cells showed increased superoxide dismutase activity compared to control GpL1 cells. These results suggest that the anti-oxidative activity of PrP(C) functions in not only neuronal cells but also astroglial cells possibly due to the increased anti-oxidative activity of astroglial cells.


Nishimura T,Sakudo A,Xue G,Ikuta K,Yukawa M,Sugiura K,Onodera T




Has Abstract


2008-12-26 00:00:00














  • Pre-pore oligomer formation by Vibrio cholerae cytolysin: insights from a truncated variant lacking the pore-forming pre-stem loop.

    abstract::Vibrio cholerae cytolysin (VCC), a β-barrel pore-forming toxin (β-PFT), induces killing of the target eukaryotic cells by forming heptameric transmembrane β-barrel pores. Consistent with the β-PFT mode of action, binding of the VCC toxin monomers with the target cell membrane triggers formation of pre-pore oligomeric ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Paul K,Chattopadhyay K

    更新日期:2014-01-03 00:00:00

  • Isolation and characterization of monoclonal antibodies against ribonuclease inhibitor.

    abstract::Mouse monoclonal antibodies were generated against human ribonuclease inhibitor, an intracellular regulatory protein. A total of four antibodies were isolated, all of which were of the immunoglobulin G1 subtype. Western blot analysis of the antibodies suggested monospecificity. Based on immunoradiometric competition a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Feldman M,Kohtz DS,Kleinberg DL

    更新日期:1988-11-30 00:00:00

  • Increased antiviral and opsonic activity of a highly multimerized collectin chimera.

    abstract::Altering the carbohydrate binding properties of surfactant protein D (SP-D) [e.g., by replacing its carbohydrate recognition domain (CRD) with that of either mannose binding lectin (MBL) or conglutinin] can increase its activity against influenza A virus (IAV). The current study demonstrates that the degree of multime...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: White MR,Crouch E,Chang D,Hartshorn KL

    更新日期:2001-08-10 00:00:00

  • Effect of a novel thermogenic beta-adrenoceptor agonist (BRL 26830) on insulin resistance in soleus muscle from obese Zucker rats.

    abstract::Young lean (Fa/?) and obese (fa/fa) rats were treated with the thermogenic beta-adrenoceptor agonist, BRL 26830, for 3 weeks. In lean rats this treatment had no effect on body weight but there was a marked increase in the insulin sensitivity of soleus muscle strips with respect to glycolytic rate. Treatment of obese r...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Challiss RA,Budohoski L,Newsholme EA,Sennitt MV,Cawthorne MA

    更新日期:1985-04-30 00:00:00

  • Inhibition of lysine-specific demethylase 1 by the acyclic diterpenoid geranylgeranoic acid and its derivatives.

    abstract::Lysine-specific demethylase 1 (LSD1) is upregulated in many cancers, especially neuroblastoma. We set out to explore whether geranylgeranoic acid (GGA) inhibits LSD1 activity by using recombinant human LSD1. GGA inhibited LSD1 activity with IC50 similar to that of the clinically used drug tranylcypromine. In human neu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sakane C,Okitsu T,Wada A,Sagami H,Shidoji Y

    更新日期:2014-01-31 00:00:00

  • Genetics of subpeptin JM4-A and subpeptin JM4-B production by Bacillus subtilis JM4.

    abstract::Subpeptin JM4-A and subpeptin JM4-B are two novel antimicrobial peptides produced by Bacillus subtilis JM4. To identify putative genes involved in their production, degenerate PCR primers targeted to conserved motifs of nonribosomal peptide synthetases (NRPSs) were used. A resulting 1.2 kb PCR product had high sequenc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wu S,Zhong J,Huan L

    更新日期:2006-06-16 00:00:00

  • A CRE and the region occupied by a protein induced by growth factors contribute to up-regulation of cyclin D1 expression in hepatocytes.

    abstract::Induction of cyclin D1 expression is a critical feature of growth factor-induced cell proliferation in hepatocytes. To clarify the mechanisms regulating cyclin D1 gene expression, we isolated the rat cyclin D1 gene and analyzed the transcriptional regulatory elements in rat hepatoma cells and primary cultured rat hepa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Moriuchi A,Ido A,Nagata Y,Nagata K,Uto H,Hasuike S,Hori T,Hirono S,Hayashi K,Tsubouchi H

    更新日期:2003-01-10 00:00:00

  • Distinct characteristics of receptor-operated Ca2+ influx and refilling in pancreatic acinar cells.

    abstract::Ca2+ influx from the extracellular space in nonexcitable cells occurs via receptor-operated and refilling processes. However, they showed different characteristics with respect to the Mn2+ permeability, depletion of intracellular Ca2+ stores, and sensitivity to the K+ ionophore valinomycin in rat pancreatic acinar cel...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tsunoda Y,Tashiro Y

    更新日期:1999-03-24 00:00:00

  • PhyA gene product of Aspergillus ficuum and Peniophora lycii produces dissimilar phytases.

    abstract::PhyA gene products of Aspergillus ficuum (AF) and Peniophora lycii (PL) as expressed in industrial strains of Aspergillus niger and Aspergillus oryzae, respectively, were purified to homogeneity and then characterized for both physical and biochemical properties. The PL phytase is 26 amino acid residues shorter than t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ullah AH,Sethumadhavan K

    更新日期:2003-04-04 00:00:00

  • Internalization of MUC1 by anti-MUC1 antibody from cell membrane through the macropinocytotic pathway.

    abstract::MUC1 is a type I transmembrane glycoprotein aberrantly overexpressed in various cancer cells. It is thought to serve as a physical barrier from the extracellular environment and also as a receptor for various extracellular molecules. However, little is known about the fate of MUC1 during and after the interaction with...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hisatsune A,Kawasaki M,Nakayama H,Mikami Y,Miyata T,Isohama Y,Katsuki H,Kim KC

    更新日期:2009-10-30 00:00:00

  • Transgenic expression of L-gulono-gamma-lactone oxidase in medaka (Oryzias latipes), a teleost fish that lacks this enzyme necessary for L-ascorbic acid biosynthesis.

    abstract::Transfer of the gene for L-gulono-gamma-lactone oxidase, the missing enzyme in L-ascorbic acid biosynthesis in scurvy-prone animals, into medaka (Oryzias latipes) was successfully done. The expression plasmid pSVL-GLO, carrying rat liver L-gulono-gamma-lactone oxidase cDNA, was microinjected into the cytoplasm of fert...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Toyohara H,Nakata T,Touhata K,Hashimoto H,Kinoshita M,Sakaguchi M,Nishikimi M,Yagi K,Wakamatsu Y,Ozato K

    更新日期:1996-06-25 00:00:00

  • An alpha-subunit loop structure is required for GM2 activator protein binding by beta-hexosaminidase A.

    abstract::The alpha- and/or beta-subunits of human beta-hexosaminidase A (alphabeta) and B (betabeta) are approximately 60% identical. In vivo only beta-hexosaminidase A can utilize GM2 ganglioside as a substrate, but requires the GM2 activator protein to bind GM2 ganglioside and then interact with the enzyme, placing the termi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zarghooni M,Bukovac S,Tropak M,Callahan J,Mahuran D

    更新日期:2004-11-19 00:00:00

  • Oxidative stress increases hepatocyte iNOS gene transcription and promoter activity.

    abstract::Hepatocyte expression of inducible nitric oxide synthase (iNOS) is initiated by the presence of pro-inflammatory cytokines, such as interleukin-1beta (IL-1). In the presence of oxidative stress, IL-1beta mediated hepatocyte iNOS expression and NO synthesis are significantly increased. To determine the underlying molec...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kuo PC,Abe KY,Schroeder RA

    更新日期:1997-05-19 00:00:00

  • Getting mitochondria to center stage.

    abstract::The question of how eukaryotic cells assemble their mitochondria was long considered to be inaccessible to biochemical investigation. This attitude changed about fifty years ago when the powerful tools of yeast genetics, electron microscopy and molecular biology were brought to bear on this problem. The rising interes...

    journal_title:Biochemical and biophysical research communications

    pub_type: 历史文章,杂志文章,评审


    authors: Schatz G

    更新日期:2013-05-10 00:00:00

  • Increased copper toxicity in Saccharomyces cerevisiae lacking VPS35, a component of the retromer and monogenic Parkinson disease gene in humans.

    abstract::The Saccharomyces cerevisiae gene VPS35 encodes a component of the retromer complex which is involved in vesicle transport from endosomes to the trans-Golgi network. Yeast and human VPS35 orthologs are highly conserved and mutations in human VPS35 cause an autosomal dominant form of late-onset Parkinson disease (PD). ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sowada N,Stiller B,Kubisch C

    更新日期:2016-08-05 00:00:00

  • A novel methyltransferase required for the formation of the hypermodified nucleoside wybutosine in eucaryotic tRNA.

    abstract::We demonstrate that the product of the yeast open reading frame YML005w is required for wybutosine (yW) formation in the phenylalanine-accepting tRNA of the yeast Saccharomyces cerevisiae. tRNA isolated from a deletion mutant of the YML005w gene accumulates 4-demethylwyosine (ImG-14), a precursor lacking three of the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kalhor HR,Penjwini M,Clarke S

    更新日期:2005-08-26 00:00:00

  • Production of soluble integrin alpha2beta1 heterodimer complex functionally active in vitro and in vivo.

    abstract::Integrin alpha2beta1, which is a membrane protein consisting of noncovalently bound alpha2 and beta1 chains, mediates cell binding to collagen and plays a role in platelet functions. DNAs encoding the chimeric proteins in which the extracellular domains of each alpha2 and beta1 chain was fused to hinge and Fc regions ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kainoh M,Tanaka T

    更新日期:2002-01-11 00:00:00

  • Methylation of the vitamin D-dependent CaBP gene (calbindin 9 Kd) does not mediate tissue or vitamin D regulation.

    abstract::The vitamin D-dependent intestinal calcium binding protein gene is predominantly expressed in the intestine. In this report we have examined the possibility that methylation of the gene might play a role in its tissue-specific expression employing genomic Southern analysis. None of the Hpa II and Hha I sites examined ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Darwish HM,Krisinger J,DeLuca HF

    更新日期:1989-05-15 00:00:00

  • Interleukin 1 alpha mRNA in virus-transformed T and B cells.

    abstract::IL-1 alpha cDNA clone was isolated from a T cell line infected by the human T lymphotropic retrovirus type-I (HTLV-I/ATLV). We found significant amounts of mRNA hybridizing to IL-1 alpha cDNA not only in HTLV-I-transformed T cells but also in Epstein-Barr Virus-transformed B cells. A part of IL-2 receptor inducing act...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Noma T,Nakamura T,Maeda M,Okada M,Taniguchi Y,Tagaya Y,Yaoita Y,Yodoi J,Honjo T

    更新日期:1986-08-29 00:00:00

  • Mechanism of the action of the inhibitor of reverse transcriptase.

    abstract::An inhibitor of reverse transcriptase of Moloney leukemia virus was reported previously in the cytoplasm of the cultured cells (1). In this report, the mechanism of the inhibition was examined. The inhibitory activity was completely abrogated by treatment at 55 degrees C for 20 min. Destruction of the reaction product...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rokutanda M,Watanabe ST,Maeda YY

    更新日期:1984-08-30 00:00:00

  • miR-143 decreases COX-2 mRNA stability and expression in pancreatic cancer cells.

    abstract::Small non-coding RNAs, microRNAs (miRNA), inhibit the translation or accelerate the degradation of message RNA (mRNA) by targeting the 3'-untranslated region (3'-UTR) in regulating growth and survival through gene suppression. Deregulated miRNA expression contributes to disease progression in several cancers types, in...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pham H,Rodriguez CE,Donald GW,Hertzer KM,Jung XS,Chang HH,Moro A,Reber HA,Hines OJ,Eibl G

    更新日期:2013-09-13 00:00:00

  • Functional expression of thyrotropin receptor in differentiated 3T3-L1 cells: a possible model cell line of extrathyroidal expression of thyrotropin receptor.

    abstract::Thyrotropin receptor (TSHR) in extrathyroidal tissue, especially fat tissue, is supposed to have important roles in the development of extrathyroidal manifestations of Graves' disease. However, the molecular mechanism of TSHR expression is not known. Expression of TSHR mRNA and TSH-dependent cAMP production were obser...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Haraguchi K,Shimura H,Lin L,Saito T,Endo T,Onaya T

    更新日期:1996-06-05 00:00:00

  • Hepatocellular metabolism of 4-hydroxy-2,3-nonenal is impaired in conditions of chronic cholestasis.

    abstract::4-Hydroxy-2,3-nonenal is a major aldehydic end-product of lipid peroxidation known to exert several biological and cytotoxic effects and to be produced during conditions of chronic cholestasis. Here we report that viable hepatocytes isolated from cholestatic livers of bile duct-ligated rats (BDL hepatocytes) show a si...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Leonarduzzi G,Parola M,Muzio G,Garramone A,Maggiora M,Robino G,Poli G,Dianzani MU,Canuto RA

    更新日期:1995-09-14 00:00:00

  • The inhibition of progesterone secretion and the regulation of cyclic nucleotides by atrial natriuretic factor in gonadotropin responsive murine Leydig tumor cells.

    abstract::We have found that atrial natriuretic factor (ANF) has a profound effect on testicular cells in altering intracellular cyclic nucleotide levels as well as progesterone secretion. Using clonal cultured Leydig tumor cells we found that 1 X 10(-8)M ANF caused a two thousand-fold elevation in the accumulation of cellular ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pandey KN,Kovacs WJ,Inagami T

    更新日期:1985-12-17 00:00:00

  • Deficient repair of 8-hydroxyguanine in the BxPC-3 pancreatic cancer cell line.

    abstract::Elevated levels of oxidatively induced DNA lesions have been reported in malignant pancreatic tissues relative to normal pancreatic tissues. However, the ability of the pancreatic cancer cells to remove these lesions has not previously been addressed. This study analyzed the effectiveness of the pancreatic cancer cell...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nyaga SG,Lohani A,Evans MK

    更新日期:2008-11-14 00:00:00

  • UV light induces premature senescence in Akt1-null mouse embryonic fibroblasts by increasing intracellular levels of ROS.

    abstract::Akt/PKB plays a pivotal role in cell survival and proliferation. Previously, we reported that UV-irradiation induces extensive cell death in Akt2(-/-) mouse embryonic fibroblasts (MEFs) while Akt1(-/-) MEFs show cell cycle arrest. Here, we find that Akt1(-/-) MEFs exhibit phenotypic changes characteristics of senescen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Jee HJ,Kim HJ,Kim AJ,Bae YS,Bae SS,Yun J

    更新日期:2009-06-05 00:00:00

  • Cholecystokinin-induced redistribution of paxillin in rat pancreatic acinar cells.

    abstract::Cholecystokinin (CCK) dose-dependently stimulates enzyme secretion or loss of cell integrity in the exocrine pancreas. Signaling mechanims include tyrosine phosphorylation of p125(FAK) and paxillin. Here, we examine their potential function. Maximum phosphorylation of both proteins was observed after stimulation of fr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Leser J,Lührs H,Beil MF,Adler G,Lutz MP

    更新日期:1999-01-19 00:00:00

  • Molecular cloning and nucleotide sequence of complementary DNA encoding rabbit alpha 1-acid glycoprotein.

    abstract::Alpha 1-acid glycoprotein (AGP) complementary DNA clone has been isolated from an acute phase rabbit liver library. Complete and full-length nucleotide sequence of this cDNA has been determined. The cDNA contains 35 nucleotides 5' untranslated region followed by 606 nucleotides of coding region and a 112 nucleotides 3...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ray BK,Ray A

    更新日期:1991-07-31 00:00:00

  • Resolvin E1 dampens airway inflammation and hyperresponsiveness in a murine model of asthma.

    abstract::Resolvin E1 (RvE1; 5S, 12R, 18R-trihydroxyeicosapentaenoic acid) is an anti-inflammatory lipid mediator derived from the omega-3 fatty acid eicosapentaenoic acid (EPA). It has been recently shown that RvE1 is involved in the resolution of inflammation. However, it is not known whether RvE1 is involved in the resolutio...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Aoki H,Hisada T,Ishizuka T,Utsugi M,Kawata T,Shimizu Y,Okajima F,Dobashi K,Mori M

    更新日期:2008-03-07 00:00:00

  • Inverse expression of two laminin binding proteins, 67LR and galectin-3, correlates with the invasive phenotype of trophoblastic tissue.

    abstract::Tumor invasion of host tissues and trophoblastic penetration of the endometrium share common biological features. Both processes involve the invasion of basement membranes, an event that is initiated by adhesion of cancer or trophoblast cells to basement membrane components and particularly to laminin. Adhesion to thi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: van den Brûle FA,Price J,Sobel ME,Lambotte R,Castronovo V

    更新日期:1994-05-30 00:00:00