当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Intensified autophagy compromises the efficacy of radiotherapy against prostate cancer.

Intensified autophagy compromises the efficacy of radiotherapy against prostate cancer.

Abstract：

INTRODUCTION:Radiotherapy is an equivalent alternative or complement to radical prostatectomy, with high therapeutic efficacy. High risk patients, however, experience high relapse rates, so that research on radio-sensitization is the most evident route to improve curability of this common disease. MATERIALS AND METHODS:In the current study we investigated the autophagic activity in a series of patients with localized prostate tumors treated with radical radiotherapy, using the LC3A and the LAMP2a proteins as markers of autophagosome and lysosome cellular content, respectively. The role of autophagy on prostate cancer cell line resistance to radiation was also examined. RESULTS:Using confocal microscopy on tissue biopsies, we showed that prostate cancer cells have, overall, high levels of LC3A and low levels of LAMP2a compared to normal prostate glands. Tumors with a 'highLC3A/lowLAMP2a' phenotype, suggestive of intensified lysosomal consumption, had a significantly poorer biochemical relapse free survival. The PC3 radioresistant cell line sustained remarkably its autophagic flux ability after radiation, while the DU145 radiosensitive one experiences a prolonged blockage of the autophagic process. This was assessed with aggresome accumulation detection and LC3A/LAMP2a double immunofluorescence, as well as with sequestrosome/p62 protein detection. By silencing the LC3A or LAMP2a expression, both cell lines became more sensitive to escalated doses of radiation. CONCLUSIONS:High base line autophagy activity and cell ability to sustain functional autophagy define resistance of prostate cancer cells to radiotherapy. This can be reversed by blocking up-regulated components of the autophagy pathway, which may prove of importance in the field of clinical radiotherapy.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Koukourakis MI,Kalamida D,Mitrakas A,Pouliliou S,Kalamida S,Sivridis E,Giatromanolaki A

doi

10.1016/j.bbrc.2015.04.014

subject

Has Abstract

pub_date

2015-05-29 00:00:00

pages

268-74

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(15)00680-4

journal_volume

461

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • A selective splicing variant of hepcidin mRNA in hepatocellular carcinoma cell lines.

    abstract：:Hepcidin is a main regulator of iron metabolism, of which abnormal expression affects intestinal absorption and reticuloendothelial sequestration of iron by interacting with ferroportin. It is also noted that abnormal iron accumulation is one of the key factors to facilitate promotion and progression of cancer includi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.05.153

    authors： Toki Y,Sasaki K,Tanaka H,Yamamoto M,Hatayama M,Ito S,Ikuta K,Shindo M,Hasebe T,Nakajima S,Sawada K,Fujiya M,Torimoto Y,Ohtake T,Kohgo Y

    更新日期：2016-08-05 00:00:00

  • Three growth hormone- and two prolactin-related novel peptides of Mr 13,000-18,000 identified in the anterior pituitary.

    abstract：:Electrophoretic analysis of murine anterior pituitary extract revealed five major proteins of Mr 13,000-18,000 (designated P13, P14, P16, P17, and P18 according to Mr), three of which, P16, P17, and P18, were markedly influenced by estradiol benzoate and perphenazine in a manner similar to that of growth hormone, and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80820-9

    authors： Sinha YN,Jacobsen BP

    更新日期：1988-10-14 00:00:00

  • Effects of aminoglycoside antibiotics on the coupling of protein and RNA syntheses in Escherichia coli.

    abstract：:The interdependency of protein and RNA syntheses was studied comparatively in bacteria confronted with amino acid starvation or treated separately with various aminoglycoside antibiotics. By contrast with the concomitant inhibition of macromolecular syntheses in cells deprived of an essential amino acid, RNA productio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91688-1

    authors： Cortay JC,Cozzone AJ

    更新日期：1983-05-16 00:00:00

  • Aβ1-40 mediated aggregation of proteins and metabolites unveils the relevance of amyloid cross-seeding in amyloidogenesis.

    abstract：:The multicomponent nature of neuronal plaques in Alzheimer's disease signifies the possible recruitment of non-Aβ candidates during the amyloid growth of Aβ peptides. Here, we show that amyloid fibrils of Aβ1-40 peptide can effectively initiate amyloid formation in different globular proteins and metabolites, converti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.04.198

    authors： Anand BG,Prajapati KP,Kar K

    更新日期：2018-06-18 00:00:00

  • Functional interaction between nonreceptor tyrosine kinase c-Abl and SR-Rich protein RBM39.

    abstract：:RBM39, also known as splicing factor HCC1.4, acts as a transcriptional coactivator for the steroid nuclear receptors JUN/AP-1, ESR1/ER-α and ESR2/ER-β. RBM39 is involved in the regulation of the transcriptional responses of these steroid nuclear receptors and promotes transcriptional initiation. In this paper, we repo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.03.108

    authors： Mai S,Qu X,Li P,Ma Q,Liu X,Cao C

    更新日期：2016-04-22 00:00:00

  • Inhibition of Drp1 hyperactivation reduces neuropathology and behavioral deficits in zQ175 knock-in mouse model of Huntington's disease.

    abstract：:Mitochondrial dysfunction manifests in the pathogenesis of Huntington's disease (HD), a fatal and inherited neurodegenerative disease. Dynamin-related protein 1 (Drp1) is the primary component of mitochondrial fission and becomes hyperactivated in various models of HD. We previously reported that inhibition of Drp1 hy...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.11.031

    authors： Zhao Y,Sun X,Qi X

    更新日期：2018-12-09 00:00:00

  • Molecular monitoring of bleomycin-induced pulmonary fibrosis by cDNA microarray-based gene expression profiling.

    abstract：:Pulmonary fibrosis is a progressive disorder whose molecular pathology is poorly understood. Here we developed an in-house cDNA microarray ("lung chip") originating from a lung-normalized cDNA library. By using this lung chip, we analyzed global gene expression in a murine model of bleomycin-induced fibrosis and selec...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5853

    authors： Katsuma S,Nishi K,Tanigawara K,Ikawa H,Shiojima S,Takagaki K,Kaminishi Y,Suzuki Y,Hirasawa A,Ohgi T,Yano J,Murakami Y,Tsujimoto G

    更新日期：2001-11-09 00:00:00

  • Camptothecin and Zeocin can increase p53 levels during all cell cycle stages.

    abstract：:The ability of DNA damage to stabilize p53 in all cell cycle stages has not been examined in actively growing cells. The chemotherapeutic drug camptothecin is a topoisomerase I poison. Zeocin is a member of the bleomycin/phleomycin family of antibiotics, known to bind DNA. Both increase the level of p53 albeit by diff...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6073

    authors： Houser S,Koshlatyi S,Lu T,Gopen T,Bargonetti J

    更新日期：2001-12-21 00:00:00

  • Mechanism of the action of the inhibitor of reverse transcriptase.

    abstract：:An inhibitor of reverse transcriptase of Moloney leukemia virus was reported previously in the cytoplasm of the cultured cells (1). In this report, the mechanism of the inhibition was examined. The inhibitory activity was completely abrogated by treatment at 55 degrees C for 20 min. Destruction of the reaction product...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90412-1

    authors： Rokutanda M,Watanabe ST,Maeda YY

    更新日期：1984-08-30 00:00:00

  • Proteoglycan gene expression in rat liver after partial hepatectomy.

    abstract：:Liver regeneration is regulated by several factors. Among these extracellular matrix plays a crucial role in the restoration of normal structure. Proteoglycans are major components of extracellular matrix and they are able to bind growth factors implicated in liver growth. We studied syndecan, perlecan, fibroglycan, a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1718

    authors： Gallai M,Sebestyén A,Nagy P,Kovalszky I,Onody T,Thorgeirsson SS

    更新日期：1996-11-21 00:00:00

  • Pyruvate kinase M2 promotes the growth of gastric cancer cells via regulation of Bcl-xL expression at transcriptional level.

    abstract：:PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase that promotes aerobic glycolysis. Here, we describe an important role for PKM2 in regulating the survival of gastric cancer (GC) cells. We showed that PKM2 was overexpressed in gastric tumor tissues compared to normal tissues and its expression level was as...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.05.063

    authors： Kwon OH,Kang TW,Kim JH,Kim M,Noh SM,Song KS,Yoo HS,Kim WH,Xie Z,Pocalyko D,Kim SY,Kim YS

    更新日期：2012-06-22 00:00:00

  • Mitogen-induced topoisomerase II synthesis precedes DNA synthesis in human breast cancer cells.

    abstract：:Cells released from quiescence exhibit increased levels of the DNA-modifying enzyme topoisomerase II, a nuclear protein which is also a target for antitumour drugs such as VP-16 (etoposide) and m-AMSA (4',9'-acridinylamino-methanesulfon-m-anisidide). By using Western blotting, DNA-protein crosslinking and drug-induced...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91613-6

    authors： Epstein RJ,Smith PJ

    更新日期：1989-04-14 00:00:00

  • A cold-regulated Arabidopsis gene encodes a polypeptide having potent cryoprotective activity.

    abstract：:We previously showed that cor15, a cold-regulated gene from Arabidopsis thaliana, encodes a 15 kDa polypeptide designated COR15. Here we report that COR15 has potent cryoprotective activity in an in vitro cryoprotection assay. Specifically, COR15 was very effective in protecting the cold-labile enzyme lactate dehydrog...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80304-3

    authors： Lin C,Thomashow MF

    更新日期：1992-03-31 00:00:00

  • Computational studies of the binding mechanism of calmodulin with chrysin.

    abstract：:Calmodulin (CaM) plays a crucial role in metabolism and physiology of eukaryotes by regulating biological activities. Multiple lines of evidences indicate that the phosphorylated flavonoids possess relatively stronger affinities for proteins by forming non-covalent complexes with them, and that the cellular functions ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.05.053

    authors： Li L,Wei DQ,Wang JF,Chou KC

    更新日期：2007-07-13 00:00:00

  • Efficient singlet oxygen inactivation of firefly luciferase.

    abstract：:Firefly luciferase is inactivated by singlet oxygen at near diffusion controlled rates, 1.9 X 10(9) M-1 s-1, based on direct comparison with the oxidation of L-histidine. The inactivation kinetics are multiphasic. Inactivation is inhibitable by NaN3. Surface-separated-sensitizer (SSS) system in which singlet oxygen is...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90718-7

    authors： Thompson A,Nigro J,Seliger HH

    更新日期：1986-11-14 00:00:00

  • PTHrP promotes malignancy of human oral cancer cell downstream of the EGFR signaling.

    abstract：:Parathyroid hormone-related protein (PTHrP) is detected in many aggressive tumors and involved in malignant conversion; however, the underlying mechanism remains obscure. Here, we identified PTHrP as a mediator of epidermal growth factor receptor (EGFR) signaling to promote the malignancies of oral cancers. PTHrP mRNA...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.01.121

    authors： Yamada T,Tsuda M,Ohba Y,Kawaguchi H,Totsuka Y,Shindoh M

    更新日期：2008-04-11 00:00:00

  • Cellular signaling mediated by calphoglin-induced activation of IPP and PGM.

    abstract：:Universal protein networks conserved from bacteria to animals dictate the core functions of cells. Inorganic pyrophosphatase (IPP) is an essential enzyme that plays a pivotal role in a broad spectrum of cellular biosynthetic reactions such as amino acid, nucleotide, polysaccharide, and fatty acid biosynthesis. However...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.10.021

    authors： Takahashi K,Inuzuka M,Ingi T

    更新日期：2004-12-03 00:00:00

  • Differences in mitogenic responses to angiotensin II, calf serum and phorbol ester in vascular smooth muscle cells from two strains of genetically hypertensive rat.

    abstract：:Vascular smooth muscle cells cultured from spontaneously hypertensive (SHR), New Zealand genetically hypertensive (GH) rat strains and their control strains (Wistar-Kyoto (WKY) and normal Wistar (N) rats) were compared for mitogenic responses [( 3H]-thymidine incorporation) to angiotensin II (AII), fetal calf serum (F...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90528-u

    authors： Harris EL,Grigor MR,Millar JA

    更新日期：1990-08-16 00:00:00

  • Appropriate dose of ethanol exerts anti-senescence and anti-atherosclerosis protective effects by activating ALDH2.

    abstract：:Moderate alcohol consumption has been shown to reduce atherosclerosis-associated diseases. As shown in our earlier works, ethanol has a dose-dependent protective effects against endothelial cellular senescence by activating aldehyde dehydrogenase 2 (ALDH2) in vitro. However, whether ethanol administration possesses an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.03.037

    authors： Xue L,Zhu W,Yang F,Dai S,Han Z,Xu F,Guo P,Chen Y

    更新日期：2019-04-30 00:00:00

  • NF-κB-dependent RANKL expression in a mouse model of immature T-cell leukemia.

    abstract：:Activation of the receptor activator of nuclear factor-κB (RANK) by its ligand (RANKL) is involved in both solid and hematological malignancies, including multiple myeloma, acute myeloid leukemia and B-cell leukemia. Although RANKL expression has been described in normal T cells, a potential role in T-cell leukemia re...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.01.089

    authors： Fernandes MT,Caroço LS,Pacheco-Leyva I,Dos Santos NR

    更新日期：2019-03-05 00:00:00

  • Molecular characterization of seizure-related genes isolated by differential screening.

    abstract：:To isolate seizure-related genes, we applied differential screening technique to the cDNA library which constructed from primary cultured cerebral cortical cells treated with pentylenetetrazol (PTZ). Northern blotting analysis of mRNA levels in the cerebra after systemic administration of PTZ confirmed the results of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0313

    authors： Kajiwara K,Nagawawa H,Shimizu-Nishikawa S,Ookuri T,Kimura M,Sugaya E

    更新日期：1996-02-27 00:00:00

  • Red wine polyphenols cause endothelium-dependent EDHF-mediated relaxations in porcine coronary arteries via a redox-sensitive mechanism.

    abstract：:Moderate consumption of wine is associated with cardiovascular protection most likely by increasing the endothelial formation of nitric oxide (NO). The present study investigated whether red wine polyphenolic compounds (RWPCs) increase the formation of endothelium-derived hyperpolarizing factor (EDHF) in arteries and,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.09.028

    authors： Ndiaye M,Chataigneau T,Andriantsitohaina R,Stoclet JC,Schini-Kerth VB

    更新日期：2003-10-17 00:00:00

  • The binding of N-(phosphonacetyl)-L-aspartate to aspartate carbamoyltransferase of Escherichia coli.

    abstract：:A more precise description of the binding of N-(phosphonacetyl)-L- aspartate to the catalytic chains of aspartate carbamoyltransferase clarifies aspects of the specificity of this enzyme toward its substrates, carbamoylphosphate and L-aspartate, and suggests a catalytic role for His-134. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90514-0

    authors： Volź KW,Krause KL,Lipscomb WN

    更新日期：1986-04-29 00:00:00

  • p-Coumaric acid stimulates longitudinal bone growth through increasing the serum production and expression levels of insulin-like growth factor 1 in rats.

    abstract：:The aim of the present study was to examine the effects of p-coumaric acid on the longitudinal growth of the long bone in adolescent male rats. Teatment with p-coumaric acid significantly increased the tibial length and the height of each growth plate zone and the ratio of 5-bromo-2'-deoxyuridine-positive cells relati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.10.046

    authors： Lee JH,Chung YH,Kim HH,Bang JS,Jung TW,Park T,Park J,Kim U,Lee SH,Jeong JH

    更新日期：2018-11-10 00:00:00

  • MicroRNA-21 accelerates hepatocyte proliferation in vitro via PI3K/Akt signaling by targeting PTEN.

    abstract：:MicroRNAs (miRNAs) are involved in controlling hepatocyte proliferation during liver regeneration. In this study, we established the miRNAs-expression patterns of primary hepatocytes in vitro under stimulation of epidermal growth factor (EGF), and found that microRNA-21 (miR-21) was appreciably up-regulated and peaked...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.12.047

    authors： Yan-nan B,Zhao-yan Y,Li-xi L,jiang Y,Qing-jie X,Yong Z

    更新日期：2014-01-17 00:00:00

  • Potent inhibition of tau fibrillization with a multivalent ligand.

    abstract：:Small-molecule inhibitors of tau fibrillization are under investigation as tools for interrogating the tau aggregation pathway and as potential therapeutic agents for Alzheimer's disease. Established inhibitors include thiacarbocyanine dyes, which can inhibit recombinant tau fibrillization in the presence of anionic s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.08.166

    authors： Honson NS,Jensen JR,Darby MV,Kuret J

    更新日期：2007-11-09 00:00:00

  • The retinoic acid and brain-derived neurotrophic factor differentiated SH-SY5Y cell line as a model for Alzheimer's disease-like tau phosphorylation.

    abstract：:The paired helical filaments of highly phosphorylated tau protein are the main components of neurofibrillary tangles (NFT) in Alzheimer's disease (AD). Protein kinases including glycogen synthase kinase 3 beta (GSK3beta), cyclin-dependent kinase 5 (Cdk5), and c-Jun N-terminal kinase (JNK) have been implicated in NFT f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.05.075

    authors： Jämsä A,Hasslund K,Cowburn RF,Bäckström A,Vasänge M

    更新日期：2004-07-02 00:00:00

  • A phytoene desaturase homolog gene from the methanogenic archaeon Methanosarcina acetivorans is responsible for hydroxyarchaeol biosynthesis.

    abstract：:Hydroxyarchaeols are the typical core structures of archaeal membrane lipids uniquely produced by a limited number of methanogenic lineages, which are mainly classified in orders Methanosarcinales and Methanococcales. However, the biosynthetic machinery that is used for the biosynthesis of hydroxyarcheol core lipids h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.09.001

    authors： Mori T,Isobe K,Ogawa T,Yoshimura T,Hemmi H

    更新日期：2015-10-16 00:00:00

  • Inhibitory regulation of EGF receptor degradation by sorting nexin 5.

    abstract：:Endosomal trafficking of EGF receptor (EGFR) upon stimulation is a highly regulated process during receptor-mediated signaling. Recently, the sorting nexin (SNX) family has emerged as an important regulator in the membrane trafficking of EGFR. Here, we report the identification of a novel interaction between two membe...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.01.179

    authors： Liu H,Liu ZQ,Chen CX,Magill S,Jiang Y,Liu YJ

    更新日期：2006-04-07 00:00:00

  • Stress-induced p53 runs a transcription-independent death program.

    abstract：:The transcription-independent p53-mediated apoptotic response has obtained a solid mechanistic basis in recent years. A fraction of stress-induced wild type p53 protein rapidly translocates to mitochondria in response to genotoxic, hypoxic, and oxidative stresses in established cell lines and primary cells, as well as...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2005.03.187

    authors： Erster S,Moll UM

    更新日期：2005-06-10 00:00:00