Efficient singlet oxygen inactivation of firefly luciferase.

abstract：:Primary mouse embryo fibroblasts passed in culture go through a period of declining growth rate and then after a period of minimal proliferation begin to grow again, generating, under appropriate conditions, an immortal 3T3 line. The 3T3 cells, but not the primary mouse embryo fibroblasts, are able to synthesize and t...

journal_title：Biochemical and biophysical research communications

authors： Edwards DR,Parfett CL,Smith JH,Denhardt DT

更新日期：1987-08-31 00:00:00

abstract：:Sulfation is an important pathway in the metabolism of thyroid hormones. Sulfated iodothyronines are elevated in nonthyroidal illnesses and in the normal human fetal circulation. We assayed and characterized COS-1 cell expressed recombinant human liver dehydroepiandrosterone sulfotransferase (DHEA ST or SULT2A1) and e...

journal_title：Biochemical and biophysical research communications

authors： Li X,Anderson RJ

更新日期：1999-10-05 00:00:00

abstract：:Akt kinase-interacting protein 1 (Aki1)/Freud-1/CC2D1A is localized in the cytosol, nucleus, and centrosome. Aki1 plays distinct roles depending on its localization. In the cytosol, it acts as a scaffold protein in the phosphoinositide 3-kinase (PI3K)/3-phosphoinositide-dependent protein kinase 1 (PDK1)/Akt pathway. I...

journal_title：Biochemical and biophysical research communications

authors： Nakamura A,Naito M,Arai H,Fujita N

更新日期：2010-03-19 00:00:00

abstract：:maf is a family of oncogenes, originally identified from the avian oncogenic retrovirus AS42, which encodes a nuclear bZip transcription factor protein. It has been reported that maf family genes have critical roles in embryological development and cellular differentiation. In this study, the distribution of maf-1 gen...

journal_title：Biochemical and biophysical research communications

authors： Imaki J,Onodera H,Tsuchiya K,Imaki T,Mochizuki T,Mishima T,Yamashita K,Yoshida K,Sakai M

更新日期：2000-06-16 00:00:00

abstract：:Spinal and bulbar muscular atrophy (SBMA) is one of a group of human inherited neurodegenerative diseases caused by polyglutamine expansion. There is increasing evidence that generation of truncated proteins containing an expanded polyglutamine tract may be an important step in the pathogenesis of these disorders. We ...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi Y,Miwa S,Merry DE,Kume A,Mei L,Doyu M,Sobue G

更新日期：1998-11-09 00:00:00

abstract：:Cilia are microtubule-based organelles that are present on the surfaces of almost all vertebrate cells. Most cilia function as sensory or molecular transport structures. Malfunctions of cilia have been implicated in several diseases of human development. The assembly of cilia is initiated by the centriole (or basal bo...

journal_title：Biochemical and biophysical research communications

authors： Nagai Y,Asaoka Y,Namae M,Saito K,Momose H,Mitani H,Furutani-Seiki M,Katada T,Nishina H

更新日期：2010-06-11 00:00:00

abstract：:Bacterial strains were isolated from the pig colon to screen for phytase and acid phosphatase activities. Among 93 colonies, Colony 88 had the highest activities for both enzymes and was identified as an Escherichia coli strain. Using primers derived from the E. coli pH 2.5 acid phosphatase appA sequence (Dassa et al....

journal_title：Biochemical and biophysical research communications

authors： Rodriguez E,Han Y,Lei XG

更新日期：1999-04-02 00:00:00

abstract：:Acivicin, a chlorinated amino acid antibiotic, is found to be remarkably effective in killing both the vector and the host form of the parasitic protozoa, Leishmania donovani, the causative agent for visceral leishmaniasis or Kala-azar. The ED50 (50 nM) for the pathogenic amastigote form in in vitro screening system i...

journal_title：Biochemical and biophysical research communications

authors： Mukherjee T,Roy K,Bhaduri A

更新日期：1990-07-31 00:00:00

abstract：:Epidemiologic studies suggest that low to moderate consumption of red wine is inversely associated with the risk of coronary heart disease; the protection is in part attributed to grape-derived polyphenols, notably trans-resveratrol, present in red wine. It is not clear whether the cardioprotective effects of resverat...

journal_title：Biochemical and biophysical research communications

authors： Wang Z,Chen Y,Labinskyy N,Hsieh TC,Ungvari Z,Wu JM

更新日期：2006-07-21 00:00:00

abstract：:Fifteen luciferase plasmid constructs driven by various promoters including cytomegalovirus (CMV), Rous sarcoma virus (RSV), human serum albumin (SA), alpha-1 antitrypsin (AAT), cytochrome P450 CYP1A2, CYP2C9, CYP2C18, CYP2D6, CYP3A4, mouse CYP2b10, human amyloid precursor protein (APP), chicken beta actin (ACT), nucl...

journal_title：Biochemical and biophysical research communications

authors： Al-Dosari M,Zhang G,Knapp JE,Liu D

更新日期：2006-01-13 00:00:00

abstract：:We quantified the damage yields produced in plasmid DNA by γ-irradiation in the presence of low concentrations (10-100 μM) of ascorbic acid, which is a major antioxidant in living systems, to clarify whether it chemically repairs radiation damage in DNA. The yield of DNA single strand breaks induced by irradiation was...

journal_title：Biochemical and biophysical research communications

authors： Hata K,Urushibara A,Yamashita S,Shikazono N,Yokoya A,Katsumura Y

更新日期：2013-05-03 00:00:00

abstract：:The cytosolic sulfotransferases (SULTs) in vertebrates catalyze the sulfonation of endogenous thyroid/steroid hormones and catecholamine neurotransmitters, as well as a variety of xenobiotics, using 3'-phosphoadenosine 5'-phosphosulfate (PAPS) as the sulfonate donor. In this study, we determined the structures of SULT...

journal_title：Biochemical and biophysical research communications

authors： Lu J,Li H,Zhang J,Li M,Liu MY,An X,Liu MC,Chang W

更新日期：2010-05-28 00:00:00

abstract：:Targeting mitochondrial respiration has been documented as an effective therapeutic strategy in cancer. However, the impact of mitochondrial respiration inhibition on cervical cancer cells are not well elucidated. Using a panel of cervical cancer cell lines, we show that an existing drug atovaquone is active against t...

journal_title：Biochemical and biophysical research communications

authors： Tian S,Chen H,Tan W

更新日期：2018-05-23 00:00:00

abstract：:It has been well documented that tumor suppressor p53 is mutated in about 50% of all human tumors. p53 status might be one of the critical determinants for the chemo-sensitivity of human tumors. In the present study, we have found that p53 family member p73 as well as 14-3-3sigma is down-regulated in response to adria...

journal_title：Biochemical and biophysical research communications

authors： Sang M,Li Y,Ozaki T,Ono S,Ando K,Yamamoto H,Koda T,Geng C,Nakagawara A

更新日期：2006-08-18 00:00:00

abstract：:Various tumor cells express interleukin 7 (IL-7) and IL-7 variants. IL-7 has been confirmed to stimulate solid tumor cell proliferation. However, the effect of IL-7 variants on tumor cell proliferation remains unclear. In this study, we evaluated the role of IL-7δ5 (an IL-7 variant lacking exon 5) on proliferation and...

journal_title：Biochemical and biophysical research communications

authors： Pan D,Liu B,Jin X,Zhu J

更新日期：2012-06-15 00:00:00

abstract：:ShcA proteins mediate Erk1/Erk2 activation by integrins and epidermal growth factor (EGF), and are expressed as p46ShcA, p52ShcA, and p66ShcA. Although p52ShcA and p46ShcA mediate Erk1/Erk2 activation, p66ShcA antagonizes Erk activation. p66ShcA is spatially regulated during lung development, leading us to hypothesize...

journal_title：Biochemical and biophysical research communications

authors： Smith SM,Crowe DL,Lee MK

更新日期：2006-04-14 00:00:00

abstract：:p105 plays dual roles in NF-kappaB signaling: in its precursor form it inhibits NF-kappaB activation, but limited processing by the ubiquitin system generates the p50 active subunit of the transcription factor. Here we show that ABIN-1, an A20-binding protein that is also known to attenuate NF-kappaB activation, inhib...

journal_title：Biochemical and biophysical research communications

authors： Cohen S,Ciechanover A,Kravtsova-Ivantsiv Y,Lapid D,Lahav-Baratz S

更新日期：2009-11-13 00:00:00

abstract：:GPR35, previously an orphan G-protein coupled receptor, is a receptor for kynurenic acid. Here we examine the distribution of GPR35 in the rat dorsal root ganglion (DRG) and the effects of its selective activation. GPR35 was expressed predominantly by small- to medium-diameter neurons of the DRG. Many of these same ne...

journal_title：Biochemical and biophysical research communications

authors： Ohshiro H,Tonai-Kachi H,Ichikawa K

更新日期：2008-01-11 00:00:00

abstract：:Upon incubation of bovine thyroglobulin with trypsin at high enzyme/substrate ratio, some fragments with apparent masses between 18 and 31 kDa resisted prolonged digestion. Their NH2-terminal sequences were determined. All fragments overlapped with some of the Cys-rich repeats that compose a large part of thyroglobuli...

journal_title：Biochemical and biophysical research communications

authors： Gentile F,Palumbo G

更新日期：1993-11-15 00:00:00

abstract：:12-O-tetradecanoyl phorbol 13-acetate (TPA) and 1,2-dioctanoyl-sn-glycerol (diC8) activate protein kinase C (PKC) in transformed fetal bovine aortic endothelial GM 7373 cells. Both molecules cause a similar increase in membrane-associated PKC activity and in the phosphorylation of a PKC-specific endogenous 87-kDa subs...

journal_title：Biochemical and biophysical research communications

authors： Maier JA,Presta M,Ragnotti G

更新日期：1989-04-28 00:00:00

abstract：:Gemin4 is a ubiquitously expressed multifunctional protein that is involved in U snRNP assembly, apoptosis, nuclear/cytoplasmic transportation, transcription, and RNAi pathways. Gemin4 is one of the core components of the Gemin-complex, which also contains survival motor neuron (SMN), the seven Gemin proteins (Gemin2-...

journal_title：Biochemical and biophysical research communications

authors： Lorson MA,Dickson AM,Shaw DJ,Todd AG,Young EC,Morse R,Wolstencroft C,Lorson CL,Young PJ

更新日期：2008-10-10 00:00:00

abstract：:The aim of this work was to characterize folates modified by ONOO(-) and HOCl and to evaluate the binding capacity of folates modified by ONOO(-) to folate receptor alpha and beta. For the modification of folate by ONOO(-), folic acid was reacted with the combination of PMA activated PMN and PAPA NONOate or chemically...

journal_title：Biochemical and biophysical research communications

authors： Nakamura M,Nagayoshi R,Ijiri K,Nakashima-Matsushita N,Takeuchi T,Matsuyama T

更新日期：2002-10-11 00:00:00

abstract：:Alpha-/beta-dystroglycans (DG) located at the outmost layer of myelin sheath play a critical role in its formation and stability in the peripheral nerve system. The demyelination of nerve fibers is present in autoimmune neuritis, however, it is not known about the molecular mechanisms underlying this pathological proc...

journal_title：Biochemical and biophysical research communications

authors： Zhao XL,Li GZ,Sun B,Zhang ZL,Yin YH,Tian YS,Li H,Li HL,Wang de S,Zhong D

更新日期：2010-02-19 00:00:00

abstract：:Saccharomyces cerevisiae uso1-1 mutant stops the transport of secretory proteins from the endoplasmic reticulum to the Golgi apparatus at 37 degrees C. We found that this temperature-sensitive defect was suppressed either by increasing the concentration of calcium ion in the medium or by introducing in the cell the SL...

journal_title：Biochemical and biophysical research communications

authors： Kito M,Seog DH,Igarashi K,Kambe-Honjo H,Yoda K,Yamasaki M

更新日期：1996-03-27 00:00:00

abstract：:In this study, we demonstrate that in addition to T lymphocytes, human naïve eosinophils and the differentiated eosinophil-like cell line, AML14.3D10 express CCR8 and respond to CCL1 through CCR8 engagement. The responsiveness of cells was dependent on maturation stage, since CCL1 induced pronounced chemotaxis only in...

journal_title：Biochemical and biophysical research communications

authors： Karlsson AK,Walles K,Bladh H,Connolly S,Skrinjar M,Rosendahl A

更新日期：2011-04-22 00:00:00

abstract：:The first several months of life are a critical period for neuronal plasticity in the visual cortex, during which anatomical and physiological development depends upon visual experience. Rearing in darkness slows the time course of this critical period, such that at 5 weeks normal cats are more plastic than dark-reare...

journal_title：Biochemical and biophysical research communications

authors： Yang C,Silver B,Ellis SR,Mower GD

更新日期：2001-10-12 00:00:00

abstract：:Bone marrow mesenchymal stem cells (BMMSCs) are pluripotent stem cells, and the osteogenic differentiation of BMMSCs has been drawing attention for a long time. Bone formation is regulated by numerous molecular and cellular signaling pathways, and the differentiation of BMMSCs is controlled by a well-defined genetic p...

journal_title：Biochemical and biophysical research communications

authors： Li X,Guo L,Liu Y,Su Y,Xie Y,Du J,Zhou J,Ding G,Wang H,Bai Y,Liu Y

更新日期：2017-11-18 00:00:00

abstract：:In quiescent cultures of rabbit aortic smooth muscle cells, whole blood serum-induced DNA synthesis was inhibited markedly by protein kinase C-activating 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and phorbol-12, 13-dibutyrate (PDBu), cyclic AMP-derivatives, such as dibutyryl cyclic AMP (Bt2cAMP) and 8-bromo-cyclic A...

journal_title：Biochemical and biophysical research communications

authors： Fukumoto Y,Kawahara Y,Kariya K,Araki S,Fukuzaki H,Takai Y

更新日期：1988-11-30 00:00:00

abstract：:The MoFe protein of the complex metalloenzyme nitrogenase folds as a heterotetramer containing two copies each of the homologous alpha and beta subunits, encoded by the nifD and the nifK genes respectively. Recently, the functional expression of a fusion NifD-K protein of nitrogenase was demonstrated in Azotobacter vi...

journal_title：Biochemical and biophysical research communications

authors： Lahiri S,Pulakat L,Gavini N

更新日期：2005-11-18 00:00:00

abstract：:To elucidate whether p21waf-1/cip-1/sdi-1 expression is associated with loss of growth potential of hepatocytes of old rats, we determined p21waf-1/cip-1/sdi-1 expression of hepatocytes from old (30 months) rats during the cell cycle in primary culture. A high level of expression of p21waf-1/cip-1/sdi-1 was detected a...

journal_title：Biochemical and biophysical research communications

authors： Sawada N,Kojima T,Obata H,Saitoh M,Isomura H,Kokai Y,Satoh M,Mori M

更新日期：1996-11-21 00:00:00