当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > MicroRNA-21 promotes osteogenesis of bone marrow mesenchymal stem cells via the Smad7-Smad1/5/8-Runx2 pathway.

MicroRNA-21 promotes osteogenesis of bone marrow mesenchymal stem cells via the Smad7-Smad1/5/8-Runx2 pathway.

Abstract：

:Bone marrow mesenchymal stem cells (BMMSCs) are pluripotent stem cells, and the osteogenic differentiation of BMMSCs has been drawing attention for a long time. Bone formation is regulated by numerous molecular and cellular signaling pathways, and the differentiation of BMMSCs is controlled by a well-defined genetic program. In the present study, we isolated BMMSCs from the bone cavities of wild-type (WT) and microRNA-21 knock-out (miR-21-KO) mice and found that miR-21 was significantly upregulated during the osteogenic differentiation of BMMSCs. Under osteoinductive conditions, ALP staining and alizarin red staining showed that the bone formation of BMMSCs from miR-21-KO mice was less than that of BMMSCs from WT mice. Consistently, RT-PCR and western blotting revealed that ALP and Runx2 expression levels in miR-21-KO mice were downregulated compared with those in WT mice. Meanwhile, the calvarial bone defects of miR-21-KO mice showed less newly formed bone than did those of WT mice. Additionally, the Smad7-Smad1/5/8-Runx2 axis showed the same tendency; Smad7 overexpression and the expression of phosphorylated Smad1/5/8 complex decreased when miR-21 was knocked down. We identified a novel mechanism by which microRNA-21 (miR-21) promotes the bone formation of BMMSCs and found that this process is regulated, in part, by the Smad7-Smad1/5/8-Runx2 pathway.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Li X,Guo L,Liu Y,Su Y,Xie Y,Du J,Zhou J,Ding G,Wang H,Bai Y,Liu Y

doi

10.1016/j.bbrc.2017.09.119

subject

Has Abstract

pub_date

2017-11-18 00:00:00

pages

928-933

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(17)31891-0

journal_volume

493

pub_type

杂志文章

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