Regulation of proliferation and gene expression in cultured human aortic smooth muscle cells by resveratrol and standardized grape extracts.


:Epidemiologic studies suggest that low to moderate consumption of red wine is inversely associated with the risk of coronary heart disease; the protection is in part attributed to grape-derived polyphenols, notably trans-resveratrol, present in red wine. It is not clear whether the cardioprotective effects of resveratrol can be reproduced by standardized grape extracts (SGE). In the present studies, we determined, using cultured human aortic smooth muscle cells (HASMC), growth and specific gene responses to resveratrol and SGE provided by the California Table Grape Commission. Suppression of HASMC proliferation by resveratrol was accompanied by a dose-dependent increase in the expression of tumor suppressor gene p53 and heat shock protein HSP27. Using resveratrol affinity chromatography and biochemical fractionation procedures, we showed by immunoblot analysis that treatment of HASMC with resveratrol increased the expression of quinone reductase I and II, and also altered their subcellular distribution. Growth of HASMC was significantly inhibited by 70% ethanolic SGE; however, gene expression patterns in various cellular compartments elicited in response to SGE were substantially different from those observed in resveratrol-treated cells. Further, SGE also differed from resveratrol in not being able to induce relaxation of rat carotid arterial rings. These results indicate that distinct mechanisms are involved in the regulation of HASMC growth and gene expression by SGE and resveratrol.


Wang Z,Chen Y,Labinskyy N,Hsieh TC,Ungvari Z,Wu JM




Has Abstract


2006-07-21 00:00:00














  • Streptozotocin-induced diabetes in the rat is associated with enhanced tissue hydrogen sulfide biosynthesis.

    abstract::This investigation is aimed to determine whether the biosynthesis of H(2)S, an endogenous vasodilator gas, is altered in the streptozotocin-diabetic rat. Plasma H(2)S concentration as well as the activity, and expression, of H(2)S synthesizing enzymes (namely cystathionine-gamma-lyase (CSE) and cystathionine-beta-synt...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yusuf M,Kwong Huat BT,Hsu A,Whiteman M,Bhatia M,Moore PK

    更新日期:2005-08-12 00:00:00

  • Cell-type-specific modulation of Hox gene expression by members of the TGF-beta superfamily: a comparison between human osteosarcoma and neuroblastoma cell lines.

    abstract::Homeobox gene expression in osteoblast-like cells was investigated using the polymerase chain reaction (PCR). A total of 13 homeobox genes was detected in U-2 OS (human osteosarcoma) and MC3T3-E1 (mouse osteoblast) cells by sequencing cloned PCR products. Using specific primers, a different pattern of Hox gene express...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kloen P,Visker MH,Olijve W,van Zoelen EJ,Boersma CJ

    更新日期:1997-04-17 00:00:00

  • Plasma albumin cysteinylation is regulated by cystathionine beta-synthase.

    abstract::High homocysteine (Hcy) levels are a well-known independent risk factor for endothelial damage in atherosclerosis. We examined whether a rat intestinal model of ischemia-reperfusion was associated with high Hcy and with the modification of plasma albumin into cysteinylated species (CysAlb). The three treatment groups ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bar-Or D,Curtis CG,Sullivan A,Rael LT,Thomas GW,Craun M,Bar-Or R,Maclean KN,Kraus JP

    更新日期:2004-12-24 00:00:00

  • Chitin oligosaccharides as elicitors of chitinase activity in melon plants.

    abstract::Chitin oligosaccharides elicited chitinase activity in melon plants. Hexamer to nonamer were the most efficient elicitors: hexamer for maximal stimulation of colorimetrically assessed chitinase activity, heptamer for maximal stimulation of radiochemically assessed chitinase activity. Chitinase elicitation was a rapid ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Roby D,Gadelle A,Toppan A

    更新日期:1987-03-30 00:00:00

  • Methamphetamine directly accelerates beating rate in cardiomyocytes by increasing Ca(2+) entry via L-type Ca(2+) channel.

    abstract::Methamphetamine induces several cardiac dysfunctions, which leads to arrhythmia, cardiac failure and sudden cardiac death. Although these cardiac alterations elicited by methamphetamine were thought to be due to an indirect action of methamphetamine, namely, an excessive catecholamine release from synaptic terminals, ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sugimoto K,Okamura K,Tanaka H,Takashima S,Ochi H,Yamamoto T,Matoba R

    更新日期:2009-12-25 00:00:00

  • Purification and characterization of mouse CYP27B1 overproduced by an Escherichia coli system coexpressing molecular chaperonins GroEL/ES.

    abstract::The expression of mouse CYP27B1 in Escherichia coli has been dramatically enhanced by coexpression of GroEL/ES. To reveal the enzymatic properties of CYP27B1, we measured its hydroxylation activity toward vitamin D3 and 1alpha-hydroxyvitamin D3 (1alpha(OH)D3) in addition to the physiological substrate 25(OH)D3. Surpri...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Uchida E,Kagawa N,Sakaki T,Urushino N,Sawada N,Kamakura M,Ohta M,Kato S,Inouye K

    更新日期:2004-10-15 00:00:00

  • p56lck phosphorylation by Ca2+/calmodulin-dependent protein kinase type II.

    abstract::Ca2+/calmodulin-dependent protein kinases are implicated in regulating the Ca2+ signaling involved in T cell activation and in thymocyte selection. One of the earliest events in signaling through the T cell antigen receptor is activation of the protein tyrosine kinase p56lck. Following T cell activation or signaling t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bland MM,McDonald OB,Carrera AC

    更新日期:1994-01-14 00:00:00

  • Rnf112 deletion protects brain against intracerebral hemorrhage (ICH) in mice by inhibiting TLR-4/NF-κB pathway.

    abstract::Intracerebral hemorrhage (ICH) is reported as a common and often fatal type of stroke accompanied with high morbidity and mortality, and it frequently results in long-lasting neurological dysfunctions. However, the pathogenesis that contributes to ICH has not been fully understood. Rnf112, also known as Znf179, is a m...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhang F,Zhang C

    更新日期:2018-12-09 00:00:00

  • PES1 regulates sensitivity of colorectal cancer cells to anticancer drugs.

    abstract::PES1 (also known as Pescadillo), a nucleolar protein, was involved in biogenesis of ribosomal RNA. Up-regulation of PES1 has been documented in some human cancers, indicating that PES1 may play some crucial roles in tumorigenesis. In our previous study, it was found that silencing of PES1 resulted in decreased prolife...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xie W,Qu L,Meng L,Liu C,Wu J,Shou C

    更新日期:2013-02-15 00:00:00

  • Human thioredoxin reactivity-structure/function relationship.

    abstract::The reactivity of human thioredoxin (HTR) was tested in several reactions. HTR was as efficient as E. coli or plant and algal thioredoxins when assayed with E. coli ribonucleotide reductase or for the reduction of insulin. On the other hand, HTR was poorly reduced by NADPH and the E. coli flavoenzyme NADPH thioredoxin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Jacquot JP,de Lamotte F,Fontecave M,Schürmann P,Decottignies P,Miginiac-Maslow M,Wollman E

    更新日期:1990-12-31 00:00:00

  • Inhibition of gamma-glutamyl hydrolases in human cells by 2-mercaptomethylglutaric acid.

    abstract::Cultured human lymphocytes and fibroblasts accumulate methotrexate during 24 hours and synthesize methotrexate polyglutamates up to the hexaglutamate, with the triglutamate predominating. In the interval after incubation with methotrexate, drug is lost, metabolites are converted to longer chain-lengths, and methotrexa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Whitehead VM,Kalman TI,Vuchich MJ

    更新日期:1987-04-14 00:00:00

  • Characterization of ADAR1-mediated modulation of gene expression.

    abstract::Conversion of adenosine into inosine in RNA molecules constitutes an important post-transcriptional mechanism for generating transcript diversity and is catalyzed by adenosine deaminases acting on RNA (ADARs). Intriguingly, we observed that the editing enzyme ADAR1 enhances reporter gene expression in a cellular, plas...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Gommans WM,Maas S

    更新日期:2008-12-05 00:00:00

  • Molecular mechanisms controlling E-cadherin expression in breast cancer.

    abstract::Disruption of cell-cell adhesion, which is essential for the maintenance of epithelial plasticity and is mediated by a class of proteins called cadherins, is an initial event in the progression of cancer. Cadherins are Ca(2+)-dependent transmembrane proteins that are associated with actin via other cytoplasmic protein...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审


    authors: Baranwal S,Alahari SK

    更新日期:2009-06-19 00:00:00

  • Homozygous deletions of the MTS1 gene are rare in non-astrocytic brain tumors.

    abstract::Several studies support a role for the multiple tumor suppressor gene (MTS1) in the malignant progression of different tumor types. In this study we have examined the status of the MTS1 gene in a variety of non-astrocytic tumors of the central nervous system. It was not possible, using multiplex PCR with primers for M...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Walker DG,Duan W,Kaye AH,Lavin MF

    更新日期:1995-06-15 00:00:00

  • Characterization of the 5' flanking region of rat glucokinase gene.

    abstract::The rat glucokinase (GK) gene containing the first exon was isolated and its 5' flanking region was characterized by the bacterial chloramphenicol acetyltransferase (CAT) assay. A transient expression assay with a series of 5' deletion constructs (-5.5 k to -48) of GK-CAT fusion genes indicated that the 5' flanking se...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Noguchi T,Takenaka M,Yamada K,Matsuda T,Hashimoto M,Tanaka T

    更新日期:1989-11-15 00:00:00

  • Lycopene suppresses ovalbumin-induced airway inflammation in a murine model of asthma.

    abstract::In this study, we attempt to determine whether lycopene regulates inflammatory mediators in the ovalbumin (OVA)-induced murine asthma model. To address this, mice were sensitized and challenged with OVA, and then treated with lycopene before the last OVA challenge. Administration of lycopene significantly alleviated t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lee CM,Chang JH,Moon DO,Choi YH,Choi IW,Park YM,Kim GY

    更新日期:2008-09-19 00:00:00

  • Biochemical characterization of recombinant phosphoglucose isomerase of Mycobacterium tuberculosis.

    abstract::Phosphoglucose isomerase (PGI) is a well-characterized ubiquitous enzyme involved in the glycolytic pathway. It catalyzes the reversible isomerization of D-glucopyranose-6-phosphate and D-fructofuranose-6-phosphate and is present in all living cells. However, there is interspecies variation at the level of the primary...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mathur D,Ahsan Z,Tiwari M,Garg LC

    更新日期:2005-11-18 00:00:00

  • A common peptide stretch among enzymes localized to the Golgi apparatus: structural similarity of Golgi-associated glycosyltransferases.

    abstract::A common peptide motif has been discovered among a series of Golgi-localized glycosyltransferases. The peptide stretch, (Ser/Thr)-X-(Glu/Gln)-(Arg/Lys), always occurs near a hydrophobic domain close to the N-terminus of these enzymes which is believed to anchor them to the membrane lipid bilayer (Paulson and Colley, J...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bendiak B

    更新日期:1990-07-31 00:00:00

  • PIH1D1, a subunit of R2TP complex, inhibits doxorubicin-induced apoptosis.

    abstract::We have previously reported that the two components of R2TP complex, RNA polymerase II-associated protein 3 (RPAP3), and Reptin, regulate apoptosis. Here we characterize another component of the complex, PIH1 domain containing protein 1 (PIH1D1). PIH1D1 interacts with both RPAP3 and Monad in HEK293 or U2OS cells. PIH1...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Inoue M,Saeki M,Egusa H,Niwa H,Kamisaki Y

    更新日期:2010-12-17 00:00:00

  • UT-B1 urea transporter plays a noble role as active water transporter in C6 glial cells.

    abstract::Since our experimental results suggest that UT-B1 functions as active water transporter against osmotic gradient in C6 glial cells, we report here for the first time the evidence for the active water transport. Exposure of C6 cells to a hyperosmotic solution containing glycerol or sucrose produced cell shrinkage due t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ogami A,Miyazaki H,Niisato N,Sugimoto T,Marunaka Y

    更新日期:2006-12-22 00:00:00

  • Molecular and physiological characterization of an invertebrate homologue of a calcitonin-related receptor.

    abstract::Calcitonin is a key hormone involved in the regulation of calcium metabolism in vertebrates. Using oligonucleotide primers derived from consensus sequences of vertebrate calcitonin receptors, we have cloned and characterized the first representative of an invertebrate calcitonin receptor from the bivalve mollusc Crass...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dubos MP,Badariotti F,Rodet F,Lelong C,Favrel P

    更新日期:2003-10-24 00:00:00

  • Involvement of the G-protein-coupled receptor 4 in RANKL expression by osteoblasts in an acidic environment.

    abstract::Osteoclast activity is enhanced in acidic environments following systemic or local inflammation. However, the regulatory mechanism of receptor activator of NF-κB ligand (RANKL) expression in osteoblasts under acidic conditions is not fully understood. In the present paper, we detected the mRNA expression of the G-prot...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Okito A,Nakahama K,Akiyama M,Ono T,Morita I

    更新日期:2015-03-06 00:00:00

  • Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association.

    abstract::Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Martherus RS,Sluiter W,Timmer ED,VanHerle SJ,Smeets HJ,Ayoubi TA

    更新日期:2010-11-12 00:00:00

  • Stabilization of glucose transporter mRNA by insulin/IGF-1 and glucose deprivation.

    abstract::Chronic exposure of L6 myocytes to insulin/IGF-1 or glucose deprivation results in an increase in the level of brain-type glucose transporter (GLUT1) mRNA. We have investigated the effects of insulin and glucose deprivation on GLUT1 mRNA stability. The half-life of GLUT1 mRNA in control cells was 2-2.5 h. Insulin incr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Maher F,Harrison LC

    更新日期:1990-08-31 00:00:00

  • shRNA driven by Pol II/T7 dual-promoter system effectively induce cell-specific RNA interference in mammalian cells.

    abstract::Although Pol III promoters synthesize shRNA and elicit RNAi efficiently, however, a major limitation is that they are constitutively expressed in all cell types. To circumvent this problem, in the present study, we described a novel shRNA vector based on Pol II/T7 dual-promoter couple system: the transcription of shRN...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Peng Y,Lu JX,Shen XF

    更新日期:2007-08-24 00:00:00

  • Rapid synthesis of DNA-cysteine conjugates for expressed protein ligation.

    abstract::We report a rapid method for the covalent modification of commercially available amino-modified DNA oligonucleotides with a cysteine moiety. The resulting DNA-cysteine conjugates are versatile reagents for the efficient preparation of covalent DNA-protein conjugates by means of expressed protein ligation (EPL). The EP...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lovrinovic M,Niemeyer CM

    更新日期:2005-09-30 00:00:00

  • Akt phosphorylation site found in human caspase-9 is absent in mouse caspase-9.

    abstract::Caspase-9 is one caspase upstream of caspase-3 and its activation is stimulated by Apaf-1/cytochrome c and inhibited by Akt signals. BAD phosphorylation by Akt is an essential step for growth factor-mediated inhibition of caspase activation. Recently, it was shown that human caspase-9 is phosphorylated by Akt and that...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fujita E,Jinbo A,Matuzaki H,Konishi H,Kikkawa U,Momoi T

    更新日期:1999-10-22 00:00:00

  • The design, synthesis, and biochemical evaluation of derivatives of biphenyl sulfamate-based compounds as novel inhibitors of estrone sulfatase.

    abstract::We report the initial results of our study into the use of a potential transition state (TS) of the reaction catalyzed by the enzyme estrone sulfatase (ES) in the design of a series of simple 4'-O-sulfamoyl-4-biphenyl-based compounds as novel inhibitors of ES. The results of the study show that these compounds are: po...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,收录出版


    authors: Ahmed S,James K,Owen CP

    更新日期:2002-05-31 00:00:00

  • P202, an interferon-inducible protein, inhibits E2F1-mediated apoptosis in prostate cancer cells.

    abstract::p202, an interferon (IFN) inducible protein, is a phosphonuclear protein involved in the regulation of cell cycle, apoptosis, and differentiation. E2F1 belongs to the E2F family of proteins that are important cell cycle regulators in promoting cell growth. On the other hand, the deregulated expression of E2F1 also tri...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yan DH,Abramian A,Li Z,Ding Y,Wen Y,Liu TJ,Hunt K

    更新日期:2003-03-28 00:00:00

  • Structural analysis of a putative SAM-dependent methyltransferase, YtqB, from Bacillus subtilis.

    abstract::S-adenosyl-L-methionine (SAM)-dependent methyltransferases (MTases) methylate diverse biological molecules using a SAM cofactor. The ytqB gene of Bacillus subtilis encodes a putative MTase and its biological function has never been characterized. To reveal the structural features and the cofactor binding mode of YtqB,...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Park SC,Song WS,Yoon SI

    更新日期:2014-04-18 00:00:00