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Identification and characterisation of a nuclear localisation signal in the SMN associated protein, Gemin4.

Abstract：

:Gemin4 is a ubiquitously expressed multifunctional protein that is involved in U snRNP assembly, apoptosis, nuclear/cytoplasmic transportation, transcription, and RNAi pathways. Gemin4 is one of the core components of the Gemin-complex, which also contains survival motor neuron (SMN), the seven Gemin proteins (Gemin2-8), and Unrip. Mutations in the SMN1 gene cause the autosomal recessive disorder spinal muscular atrophy (SMA). Although the functions assigned to Gemin4 predominantly occur in the nucleus, the mechanisms that mediate the nuclear import of Gemin4 remain unclear. Here, using a novel panel of Gemin4 constructs we identify a canonical nuclear import sequence (NLS) in the N-terminus of Gemin4. The Gemin4 NLS is necessary and independently sufficient to mediate nuclear import of Gemin4. This is the first functional NLS identified within the SMN-Gemin complex.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Lorson MA,Dickson AM,Shaw DJ,Todd AG,Young EC,Morse R,Wolstencroft C,Lorson CL,Young PJ

doi

10.1016/j.bbrc.2008.07.113

subject

Has Abstract

pub_date

2008-10-10 00:00:00

pages

33-7

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(08)01433-2

journal_volume

375

pub_type

杂志文章

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