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Determination of the complete covalent structure of the gamma 2 subunit of bovine brain G proteins by mass spectrometry.

Abstract：

:The complete covalent structure of the gamma 2 subunit of bovine brain G proteins was determined by peptide mapping using matrix-assisted laser desorption ionization and electrospray ionization mass spectrometry and by partial sequencing using tandem mass spectrometry. Fragments were identified in proteolytic digests corresponding to all predicted internal sequences of gamma 2, but not for N- or C-terminal sequences. Fragments consistent with modifications of the terminal peptides were observed, however. The presence of these postulated modifications was verified by tandem mass spectrometry. These studies define the structure of the native protein and provide a basis for comparison to expressed proteins.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Wilcox MD,Schey KL,Busman M,Hildebrandt JD

doi

10.1006/bbrc.1995.1979

subject

Has Abstract

pub_date

1995-07-17 00:00:00

pages

367-74

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(85)71979-1

journal_volume

212

pub_type

杂志文章

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