Precocious induction of malic enzyme by nutritional and hormonal factors in rat foetal hepatocyte primary cultures.

Abstract:

:Rat foetal hepatocytes in primary cultures were used as a model for the study of malic enzyme gene expression. Carbohydrates and glycolytic metabolites produced the precocious induction of the malic enzyme in foetal hepatocytes cultured in the absence of serum and hormones. Palmitate prevented this induction. Insulin and triiodothyronine produced a significant increase in the malic enzyme specific activity in all the conditions studied. A synergistic effect between the two hormones is observed only when high concentrations of glucose are present. Glucagon prevents partially the induction produced by insulin plus triiodothyronine. Both carbohydrate and hormonal inductions of malic enzyme activity are related to parallel increases in its expression, and are prevented by protein synthesis inhibitors.

authors

Fabregat I,Lorenzo M,Benito M

doi

10.1016/0006-291x(89)91346-6

subject

Has Abstract

pub_date

1989-06-30 00:00:00

pages

1028-34

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(89)91346-6

journal_volume

161

pub_type

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