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Bioluminescent system for dynamic imaging of cell and animal behavior.

Abstract：

:The current utility of bioluminescence imaging is constrained by a low photon yield that limits temporal sensitivity. Here, we describe an imaging method that uses a chemiluminescent/fluorescent protein, ffLuc-cp156, which consists of a yellow variant of Aequorea GFP and firefly luciferase. We report an improvement in photon yield by over three orders of magnitude over current bioluminescent systems. We imaged cellular movement at high resolution including neuronal growth cones and microglial cell protrusions. Transgenic ffLuc-cp156 mice enabled video-rate bioluminescence imaging of freely moving animals, which may provide a reliable assay for drug distribution in behaving animals for pre-clinical studies.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Hara-Miyauchi C,Tsuji O,Hanyu A,Okada S,Yasuda A,Fukano T,Akazawa C,Nakamura M,Imamura T,Matsuzaki Y,Okano HJ,Miyawaki A,Okano H

doi

10.1016/j.bbrc.2012.01.141

subject

Has Abstract

pub_date

2012-03-09 00:00:00

pages

188-93

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(12)00195-7

journal_volume

419

pub_type

杂志文章

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