Enhanced detection and characterization of protocatechuate 3,4-dioxygenase in Acinetobacter lwoffii K24 by proteomics using a column separation.

abstract：:The alternatively spliced isoform of the nonmuscle myosin II heavy chain B (MHC-B) with an insert of 21 amino acids at the 50- to 20-kDa junction of the globular region of myosin has been demonstrated to be expressed specifically in the central nervous system (CNS) in chicken. To explore the role of this B2 inserted i...

journal_title：Biochemical and biophysical research communications

authors： Takahashi M,Hirano T,Uchida K,Yamagishi A

更新日期：1999-05-27 00:00:00

abstract：:The effect of human SCD1 heterologous expression on cellular fatty acid synthesis was investigated in the current study. The SCD1 gene expression cassette and PGK-neomycin-selectable marker cassette were co-introduced into HEK 293 cells by electroporation, and subsequently, SCD1 expression was evaluated by fatty acid ...

journal_title：Biochemical and biophysical research communications

authors： Wu Z,Li D,Gou K

更新日期：2010-07-30 00:00:00

abstract：:The Mr approximately equal to 90,000 heat shock protein, hsp90, readily interacts with the glucocorticoid receptor to form the 9 S, non-DNA-binding receptor complex. This receptor is stabilized in cytosolic preparations by sodium molybdate. In analogy, sodium molybdate stabilizes a 9 S form of the dioxin receptor. Pol...

journal_title：Biochemical and biophysical research communications

authors： Denis M,Cuthill S,Wikström AC,Poellinger L,Gustafsson JA

更新日期：1988-09-15 00:00:00

abstract：:TorD is the private chaperone of TorA, a periplasmic respiratory molybdoenzyme of Escherichia coli. In this study, it is demonstrated that TorD is required to maintain the integrity of the twin-arginine signal sequence of the cytoplasmic TorA precursors. In the absence of TorD, 35 out of the 39 amino acid residues of ...

journal_title：Biochemical and biophysical research communications

authors： Genest O,Seduk F,Ilbert M,Méjean V,Iobbi-Nivol C

更新日期：2006-01-20 00:00:00

abstract：:During the search for ANP receptor ligands of microbial origin, we isolated a novel polysaccharide, HS-142-1, from culture broth of Aureobasidium sp. HS-142-1 inhibited [125I]-rANP binding to ANP receptor in rabbit kidney cortex membranes with an IC50 of 0.3 mu g/ml, but gave no effects on specific binding of [125I]-E...

journal_title：Biochemical and biophysical research communications

authors： Morishita Y,Sano T,Ando K,Saitoh Y,Kase H,Yamada K,Matsuda Y

更新日期：1991-05-15 00:00:00

abstract：:Brain cell-free extract greatly stimulates the polymerization rate of purified tubulin with a reduction of the nucleation period and without a significant alteration of the final assembly state. This effect is mimicked by neuroblastoma extract at 10-fold lower extract concentration, but not by excess muscle extract. C...

journal_title：Biochemical and biophysical research communications

authors： Liliom K,Wágner G,Kovács J,Comin B,Cascante M,Orosz F,Ovádi J

更新日期：1999-10-22 00:00:00

abstract：:The telomere-associated protein tankyrase 1 is a poly(ADP-ribose) polymerase and is considered to be a promising target for cancer therapy, especially for BRCA-associated cancers. However, an efficient assay system for inhibitor screening has not been established, mainly due to the difficulty of efficient preparation ...

journal_title：Biochemical and biophysical research communications

authors： Yashiroda Y,Okamoto R,Hatsugai K,Takemoto Y,Goshima N,Saito T,Hamamoto M,Sugimoto Y,Osada H,Seimiya H,Yoshida M

更新日期：2010-04-09 00:00:00

abstract：:A single bout of exercise is known to increase the insulin sensitivity of skeletal muscle; however, the underlying mechanism of this phenomenon is not fully understood. Because a single bout of exercise induces a transient increase in blood interleukin-6 (IL-6) level, we hypothesized that the enhancement of insulin se...

journal_title：Biochemical and biophysical research communications

authors： Ikeda SI,Tamura Y,Kakehi S,Sanada H,Kawamori R,Watada H

更新日期：2016-05-13 00:00:00

abstract：:Antibody-based targeted therapy of cancers requires the antibody targeting of specific molecules inducing tumor cells apoptosis or death. Angiopoietin-2 (Agn-2) and translocator protein (TSPO) are identified as potential target molecules for glioblastoma therapy. The single chain anti-Agn-2 antibody (Anag-2) and anti-...

journal_title：Biochemical and biophysical research communications

authors： Li J,Zhang Z,Lv L,Qiao H,Chen X,Zou C

更新日期：2016-04-01 00:00:00

abstract：:Human embryonic stem cells (hESC) are pluripotent, and can be directed to differentiate into different cell types for therapeutic applications. To expand hESCs, it is desirable to maintain hESC growth without differentiation. As hESC colonies grow, differentiated cells are often found at the periphery of the colonies,...

journal_title：Biochemical and biophysical research communications

authors： Lam H,Patel S,Wong J,Chu J,Lau A,Li S

更新日期：2008-08-08 00:00:00

abstract：:Nonenzymatic glycation of extracellular matrix (ECM) proteins is increased in diabetes mellitus and aging and triggers cellular events leading to an imbalance in ECM homeostasis. We studied the influence of collagen glycation on matrix metalloproteinase production by dermal fibroblasts using the model of lattice cultu...

journal_title：Biochemical and biophysical research communications

authors： Rittié L,Berton A,Monboisse JC,Hornebeck W,Gillery P

更新日期：1999-10-22 00:00:00

abstract：:Reversion of adenine auxotrophic strains of D. radiodurans by cross-links in DNA was studied. Mutation was induced in a wild-type but not in a mitomycin C sensitive mutant after irradiation with near UV (approximately 365nm) in the presence of 4,5',8-trimethylpsoralen (TMP). To get information on implication of cross-...

journal_title：Biochemical and biophysical research communications

authors： Yatagai F,Kitayama S

更新日期：1983-04-29 00:00:00

abstract：:Triple-negative breast cancer (TNBC) is a major challenge in clinical practice due to its aggressiveness and lack of targeted treatment. Cancer stem-like traits contribute to tumorigenesis and immune privilege of TNBC. However, the relationship of stemness and immunosurveillance remains unclear. Here, we demonstrate t...

journal_title：Biochemical and biophysical research communications

authors： Wang H,Gao L,Qi M,Su P,Xiong X,Zhao J,Hu J,Han B

更新日期：2021-01-22 00:00:00

abstract：:Matrix metalloproteinases (MMPs) play a vital role in vasculature response to hemodynamic stimuli via the degradation of extracellular matrix substrates. In this study, we investigated the putative role of cyclic strain-induced endothelial MMP-2 (and MMP-9) expression and release in modulating bovine aortic smooth mus...

journal_title：Biochemical and biophysical research communications

authors： von Offenberg Sweeney N,Cummins PM,Birney YA,Redmond EM,Cahill PA

更新日期：2004-07-23 00:00:00

abstract：:We previously found that p53 binds to the catalytic subunit of the p34(cdc2)/cyclin B1-kinase. In the present study we analyzed the functional consequences of this interaction. Binding of wild-type p53 to p34(cdc2)/cyclin B1 results in a significant decrease of its histone H1 kinase activity. Binding of p53 to the kin...

journal_title：Biochemical and biophysical research communications

authors： Ababneh M,Götz C,Montenarh M

更新日期：2001-05-04 00:00:00

abstract：:We have isolated and characterized the 5'-flanking region of the mouse RECK gene aiming to understand the mechanism of oncogene-mediated suppression of RECK gene expression. The upstream 52-base region was found to contain a promoter activity which is, to some extent, suppressed by the ras oncogene. This region contai...

journal_title：Biochemical and biophysical research communications

authors： Sasahara RM,Takahashi C,Noda M

更新日期：1999-11-02 00:00:00

abstract：:Members of the Ca2+/calmodulin dependent protein kinase (CaMK) family and a CaMK cascade have been identified and well characterized in the brain, but little is known about their equivalents in the heart. Thus only CaMKII and its function have been reported so far. Therefore, we purified and characterized CaMKI and Ca...

journal_title：Biochemical and biophysical research communications

authors： Uemura A,Naito Y,Matsubara T,Hotta N,Hidaka H

更新日期：1998-08-19 00:00:00

abstract：:Two genes expressing 70 kDa heat shock proteins were identified in Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two hsc70 genes were followed by semi-quantitativ...

journal_title：Biochemical and biophysical research communications

authors： Ali KS,Dorgai L,Abrahám M,Hermesz E

更新日期：2003-08-01 00:00:00

abstract：:Our earlier studies on cell adhesion to immobilized carbohydrates are extended here to a marine bacterium, Vibrio furnissii. Apparently one lectin mediates the binding of these cells to glycosides of N-acetylglucosamine, mannose, and glucose covalently linked to Agarose beads. Kinetic studies show that protein synthes...

journal_title：Biochemical and biophysical research communications

authors： Yu C,Lee AM,Roseman S

更新日期：1987-11-30 00:00:00

abstract：:IL-17 plays an important role in gut homeostasis. However, the role of IL-17F in intestinal tumorigenesis has not been addressed. Here we demonstrate that ablation of IL-17F significantly inhibits spontaneous intestinal tumorigenesis in the small intestine of Apc(Min/+) mice. IL-17F ablation decreased IL-1β and Cox-2 ...

journal_title：Biochemical and biophysical research communications

authors： Chae WJ,Bothwell AL

更新日期：2011-10-14 00:00:00

abstract：:The present study investigates the immunomodulatory activities of buckwheat polysaccharide fraction (BPF) from the seed of Fagopyrum esculentum on RAW 264.7 macrophage cell line and Cyclophosphamide-induced immunosuppressed conditions in mice models. The results of in vitro showed that treatment with 0.5-10 μg/mL of B...

journal_title：Biochemical and biophysical research communications

authors： Oh MJ,Choi HD,Ha SK,Choi I,Park HY

更新日期：2018-02-19 00:00:00

abstract：:Ca2+/calmodulin-dependent protein kinases are implicated in regulating the Ca2+ signaling involved in T cell activation and in thymocyte selection. One of the earliest events in signaling through the T cell antigen receptor is activation of the protein tyrosine kinase p56lck. Following T cell activation or signaling t...

journal_title：Biochemical and biophysical research communications

authors： Bland MM,McDonald OB,Carrera AC

更新日期：1994-01-14 00:00:00

abstract：:Synthesis of ABCA1 protein in liver is necessary for high-density lipoproteins (HDL) formation in mammals. Nuclear receptor PPARγ is known as activator of ABCA1 expression, but details of PPARγ-mediated regulation of ABCA1 at both transcriptional and post-transcriptional levels in hepatocytes have not still been well ...

journal_title：Biochemical and biophysical research communications

authors： Mogilenko DA,Shavva VS,Dizhe EB,Orlov SV,Perevozchikov AP

更新日期：2010-11-19 00:00:00

abstract：BACKGROUND:Long non-coding RNAs (lncRNAs) have been demonstrated to be intensively involved in the development of various carcinomas, including glioblastoma multiforme (GBM). However, only a few of them have been well characterized. LncRNA CASP5 have been found to be up-regulated in GBM tissues compared with normal tis...

journal_title：Biochemical and biophysical research communications

authors： Zhou Y,Dai W,Wang H,Pan H,Wang Q

更新日期：2018-06-12 00:00:00

abstract：:Ambroxol is often used as a mucolytic agent in various lung diseases. However, it is unclear how ambroxol acts on bronchial epithelial cells. To clarify the action of ambroxol, we studied the effects of ambroxol on the ion transport in human Calu-3 cells, a human submucosal serous cell line, measuring the transepithel...

journal_title：Biochemical and biophysical research communications

authors： Hasegawa I,Niisato N,Iwasaki Y,Marunaka Y

更新日期：2006-05-05 00:00:00

abstract：:The endoplasmic reticulum (ER) is an organelle in which most membrane and secretory proteins are synthesized. If these proteins are not folded correctly, unfolded proteins accumulate in the ER lumen, causing a cellular situation known as ER stress. Recently, many studies on the relationship between ER stress and disea...

journal_title：Biochemical and biophysical research communications

authors： Hosoda A,Maruyama A,Oikawa D,Oshima Y,Komachi Y,Kanai G,Sato H,Iwawaki T

更新日期：2011-02-04 00:00:00

abstract：:The human EBAG9 was previously identified as an estrogen responsive gene using CpG-genomic binding site cloning (Watanate et al., (1998) Mol. Cell. Biol. 18: 442-449). Recently it was revealed that the EBAG9 is identical with RCAS1 which is a cancer cell surface antigen implicated in immune escape. Here, we isolated a...

journal_title：Biochemical and biophysical research communications

authors： Ikeda K,Sato M,Tsutsumi O,Tsuchiya F,Tsuneizumi M,Emi M,Imoto I,Inazawa J,Muramatsu M,Inoue S

更新日期：2000-07-05 00:00:00

abstract：:Four new basic proteins were isolated from horse eosinophils and purified. The eosinophils release these proteins after permeabilization with saponin and degranulation stimulized by guanosine 5'-O-thiotriphosphate. The proteins were separated and purified on a Superose P12- and a Mono S-column by fast protein liquid c...

journal_title：Biochemical and biophysical research communications

authors： Piller K,Portmann P

更新日期：1993-04-30 00:00:00

abstract：:To examine the possibility that inter-individual differences in splicing partially explain the observed differences in CYP2D6 activity, we amplified its full-length cDNA in 96 human liver RNA samples and discovered five splice variants: intron 5 retention, intron 6 retention, intron 5 and intron 6 double retention, ex...

journal_title：Biochemical and biophysical research communications

authors： Denson J,Wu Y,Yang W,Zhang J

更新日期：2005-05-06 00:00:00

abstract：:Mitochondrial dysfunction manifests in the pathogenesis of Huntington's disease (HD), a fatal and inherited neurodegenerative disease. Dynamin-related protein 1 (Drp1) is the primary component of mitochondrial fission and becomes hyperactivated in various models of HD. We previously reported that inhibition of Drp1 hy...

journal_title：Biochemical and biophysical research communications

authors： Zhao Y,Sun X,Qi X

更新日期：2018-12-09 00:00:00