Abstract:
:We have isolated and characterized the 5'-flanking region of the mouse RECK gene aiming to understand the mechanism of oncogene-mediated suppression of RECK gene expression. The upstream 52-base region was found to contain a promoter activity which is, to some extent, suppressed by the ras oncogene. This region contains two Sp1-binding motifs, one cEBPb-binding motif, and one CAAT box. Although both of the Sp1 sites were found to associate with Sp1 as well as Sp3 proteins, ras responsiveness seems to be mediated only by the downstream Sp1 site. Our data indicate that the Sp1 motif in certain contexts can serve as a negative target for the Ras signal.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Sasahara RM,Takahashi C,Noda Mdoi
10.1006/bbrc.1999.1552subject
Has Abstractpub_date
1999-11-02 00:00:00pages
668-75issue
3eissn
0006-291Xissn
1090-2104pii
S0006-291X(99)91552-8journal_volume
264pub_type
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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