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Effective internalization of U251-MG-secreted exosomes into cancer cells and characterization of their lipid components.

Abstract：

:Exosomes, the natural vehicles of various biological molecules, have been examined in several research fields including drug delivery. Although understanding of the biological functions of exosomes has increased, how exosomes are transported between cells remains unclear. We hypothesized that cell tropism is important for effective exosomal intercellular communication and that parental cells regulate exosome movement by modulating constituent exosomal molecules. Herein, we demonstrated the strong translocation of glioblastoma-derived exosomes (U251exo) into their parental (U251) cells, breast cancer (MDA-MB-231) cells, and fibrosarcoma (HT-1080). Furthermore, disruption of proteins of U251exo by enzymatic treatment did not affect their uptake. Therefore, we focused on lipid molecules of U251exo with the expectation that they are crucial for effective incorporation of U251exo by cancer cells. Phosphatidylethanolamine was identified as a unique lipid component of U251-MG cell-derived extracellular vesicles. From these results, valuable insight is provided into the targeting of U251exo to cancer cells, which will help to develop a cancer-targeted drug delivery system.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Toda Y,Takata K,Nakagawa Y,Kawakami H,Fujioka S,Kobayashi K,Hattori Y,Kitamura Y,Akaji K,Ashihara E

doi

10.1016/j.bbrc.2014.12.015

subject

Has Abstract

pub_date

2015-01-16 00:00:00

pages

768-73

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(14)02180-9

journal_volume

456

pub_type

杂志文章

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