Multi-strand binding of nuclear factors to a repressor of mouse mammary tumor virus transcription can be distinguished kinetically.

Abstract:

:NRE1 is a DNA sequence element in the long terminal repeat of mouse mammary tumor virus that represses viral transcription in mature T cells. In addition to double-stranded binding activity, factors in Jurkat T cell nuclear extracts bind specifically to each of the two single-strands of NRE1. Here we show that binding to the three forms of NRE1 can be distinguished kinetically. The on rates for double, upper and lower-strand NRE1 binding were 1.5, 3, and 11 min, respectively. Binding was extremely stable with off-rates varying from 30 and 60 min for double and upper-strand binding to 12 h for lower-strand binding. In addition, a truncated form of NRE1 that is only bound as a double-strand was observed to have an on rate of binding of 4 min and an off rate of 4 h.

authors

Rodda DJ,Giffin W,Haché RJ

doi

10.1006/bbrc.1995.1514

subject

Has Abstract

pub_date

1995-04-06 00:00:00

pages

379-84

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(85)71514-8

journal_volume

209

pub_type

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