Abstract:
:NRE1 is a DNA sequence element in the long terminal repeat of mouse mammary tumor virus that represses viral transcription in mature T cells. In addition to double-stranded binding activity, factors in Jurkat T cell nuclear extracts bind specifically to each of the two single-strands of NRE1. Here we show that binding to the three forms of NRE1 can be distinguished kinetically. The on rates for double, upper and lower-strand NRE1 binding were 1.5, 3, and 11 min, respectively. Binding was extremely stable with off-rates varying from 30 and 60 min for double and upper-strand binding to 12 h for lower-strand binding. In addition, a truncated form of NRE1 that is only bound as a double-strand was observed to have an on rate of binding of 4 min and an off rate of 4 h.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Rodda DJ,Giffin W,Haché RJdoi
10.1006/bbrc.1995.1514subject
Has Abstractpub_date
1995-04-06 00:00:00pages
379-84issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(85)71514-8journal_volume
209pub_type
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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