Identification of PTE2, a human peroxisomal long-chain acyl-CoA thioesterase.


:Computer-based approaches identified PTE2 as a candidate human peroxisomal acyl-CoA thioesterase gene. The PTE2 gene product is highly similar to the rat cytosolic and mitochondrial thioesterases, CTE1 and MTE1, respectively, and terminates in a tripeptide sequence, serine-lysine-valine(COOH), that resembles the consensus sequence for type-1 peroxisomal targeting signals. PTE2 was targeted to peroxisomes and recombinant PTE2 showed intrinsic acyl-CoA thioesterase activity with a pH optimum of 8.5. A comparison of PTE2 and PTE1 thioesterase activities across multiple acyl-CoA substrates indicated that while PTE1 was most active on medium-chain acyl-CoAs, with little activity on long-chain acyl-CoAs, PTE2 displayed high activity on medium- and long-chain acyl-CoAs. The identification of PTE2 therefore offers an explanation for the observed long-chain acyl-CoA thioesterase activity of mammalian peroxisomes.


Jones JM,Gould SJ




Has Abstract


2000-08-18 00:00:00














  • The structure of the human LIM protein ACT gene and its expression in tumor cell lines.

    abstract::We describe the human ACT genomic and cDNA sequence which like its murine counterpart contains the defining secondary structure of the FHL (Four-and-a-Half LIM-domain) LIM-protein family. The coding region of the human ACT gene spans five exons. This distribution is very similar to the FHL1 gene and includes the arran...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Morgan MJ,Whawell SA

    更新日期:2000-07-05 00:00:00

  • GLP-1 receptor antagonist as a potential probe for pancreatic beta-cell imaging.

    abstract::We examined exendin(9-39), an antagonist of glucagon-like peptide-1 (GLP-1) receptor (GLP-1R), as a potential probe for imaging of pancreatic beta-cells. To evaluate in vitro receptor specificity, binding assay was performed using dispersed mouse islet cells. Binding assay showed competitive inhibition of [(125)I]BH-e...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mukai E,Toyoda K,Kimura H,Kawashima H,Fujimoto H,Ueda M,Temma T,Hirao K,Nagakawa K,Saji H,Inagaki N

    更新日期:2009-11-20 00:00:00

  • ATM is required for rapid degradation of cyclin D1 in response to gamma-irradiation.

    abstract::The cellular response to DNA damage induced by gamma-irradiation activates cell-cycle arrest to permit DNA repair and to prevent replication. Cyclin D1 is the key molecule for transition between the G1 and S phases of the cell-cycle, and amplification or overexpression of cyclin D1 plays pivotal roles in the developme...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Choo DW,Baek HJ,Motoyama N,Cho KH,Kim HS,Kim SS

    更新日期:2009-01-23 00:00:00

  • Human ribosomal protein L7 carries two nucleic acid-binding domains with distinct specificities.

    abstract::Protein L7 is associated with the large subunit of eukaryotic ribosomes that can act as a co-regulator of nuclear receptor-mediated transcription. In this study we show that L7 carries in addition to the known N-terminal nucleic acid-binding domain (NBD 1) a second one (NBD 2) which maps to the 50 C-terminal amino aci...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: von Mikecz A,Neu E,Krawinkel U,Hemmerich P

    更新日期:1999-05-19 00:00:00

  • Theoretical variation of the H alpha chemical shift in acetyl-glycyl-N-methylamide and oligoglycines with molecular conformation and environment.

    abstract::The sum of the magnetic anisotropy and polarization contributions to the magnetic shielding constants of the alpha protons is calculated as a function of the torsion angles about the NC alpha (phi) and C alpha C' (psi) bonds of the dipeptide. The results show that the polarization or electric field effect is several f...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Gresh N,Giessner-Prettre C

    更新日期:1990-09-28 00:00:00

  • Expression of syntaxin 4 in rat skeletal muscle and rat skeletal muscle cells in culture.

    abstract::Syntaxins are a family of membrane proteins believed to participate in docking/fusing of arriving vesicles during membrane sorting and secretion. Of the six mammalian syntaxins known, only brain syntaxin 1A/1B has been biochemically characterized in its endogenous form. Syntaxin 4 mRNA is expressed in selective tissue...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sumitani S,Ramlal T,Liu Z,Klip A

    更新日期:1995-08-15 00:00:00

  • Selective inhibition of restriction endonuclease cleavage by DNA intercalators.

    abstract::The preferred dye binding sites and the microenvironment of known nucleotide sequences within mitochondrial and plasmid pBR322 DNA was probed in a gross fashion with restriction endonucleases. The intercalating dyes, ethidium bromide and propidium iodide, do not inhibit a given restriction endonuclease equally at all ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Soslau G,Pirollo K

    更新日期:1983-09-15 00:00:00

  • Disclosure by triton X-100 of NMDA-sensitive [3H] glutamate binding sites in brain synaptic membranes.

    abstract::Pretreatment of brain synaptic membrane homogenates with Triton X-100 resulted in a drastic disclosure of [3H] glutamate (Glu) binding activity which was sensitive to one of the central Glu receptor agonists, N-methyl-D-aspartic acid (NMDA). The NMDA-sensitive binding was inversely dependent on the incubation temperat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ogita K,Yoneda Y

    更新日期:1988-06-16 00:00:00

  • Molecular cloning of human epidermal transglutaminase cDNA from keratinocytes in culture.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yamanishi K,Liew FM,Konishi K,Yasuno H,Doi H,Hirano J,Fukushima S

    更新日期:1991-03-29 00:00:00

  • Effect of naturally occurring mutations in human glycine N-methyltransferase on activity and conformation.

    abstract::Missense mutations in the enzyme, glycine N-methyltransferase (GNMT) have been shown to be one cause of persistent isolated hypermethioninaemia in humans. These mutations were first identified by metabolite analysis and were shown to be L49P, N140S, and H176N by gene sequencing. Here we report the kinetic and conforma...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Luka Z,Wagner C

    更新日期:2003-12-26 00:00:00

  • Oxidized low density lipoprotein suppresses lipopolysaccharide-induced inflammatory responses in microglia: oxidative stress acts through control of inflammation.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kim OS,Lee CS,Joe EH,Jou I

    更新日期:2006-03-31 00:00:00

  • The focal adhesion kinase amino-terminal domain localises to nuclei and intercellular junctions in HEK 293 and MDCK cells independently of tyrosine 397 and the carboxy-terminal domain.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Stewart A,Ham C,Zachary I

    更新日期:2002-11-22 00:00:00

  • Identification of the up- and down-regulated genes in vancomycin-resistant Staphylococcus aureus strains Mu3 and Mu50 by cDNA differential hybridization method.

    abstract::We previously reported the first vancomycin-resistant Staphylococcus aureus (VRSA) clinical strain, Mu50, whose cell wall is remarkably thickened resulting from the activation of cell-wall synthesis. To explore the genetic basis for the vancomycin resistance, cDNA differential hybridization was performed using RNAs ex...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kuroda M,Kuwahara-Arai K,Hiramatsu K

    更新日期:2000-03-16 00:00:00

  • Mouse thymoma cell line expresses a gluconeogenic enzyme, fructose 1,6-bisphosphatase.

    abstract::Fructose 1,6-bisphosphatase was observed in a thymic lymphoma cell line, WEH17.1 (11.5 +/- 0.8 munits/mg cytosol protein). Only a trace amount of the enzyme activity was observed in normal thymus tissue. The WEH17.1 enzyme had a pH optimum at around 7.5. The AMP-concentration giving 50% inhibition of the activity was ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mizunuma H,Tashima Y

    更新日期:1989-02-15 00:00:00

  • Post-translational self-hydroxylation: a probe for oxygen activation mechanisms in non-heme iron enzymes.

    abstract::Recent years have seen considerable evolution in our understanding of the mechanisms of oxygen activation by non-heme iron enzymes, with high-valent iron-oxo intermediates coming to the forefront as formidably potent oxidants. In the absence of substrate, the generation of vividly colored chromophores deriving from th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Farquhar ER,Koehntop KD,Emerson JP,Que L Jr

    更新日期:2005-12-09 00:00:00

  • Function of the PLZF gene in early development and self-renewal of T cells in mice.

    abstract:BACKGROUND/AIMS:The expression of transcription factor Zbtb1 is essential for the maintenance and development of various blood cells in the hematopoietic system. In the current study, we found that the total number of thymocytes in PLZF deficient mice was reduced compared with thymocytes in wild-type mice, and the numb...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Du JL,Cao X,Liu HY,Zeng Y,Yang XC,Wan XM,Chang FF,Zhao TY,Jia XY,Wang HZ,Liu J,Cai KZ,Ma ZR

    更新日期:2019-04-16 00:00:00

  • Functional characterization of the Arabidopsis universal stress protein AtUSP with an antifungal activity.

    abstract::An antifungal protein, AtUSP protein (At3g53990), was isolated from Arabidopsis thaliana leaves by ion and size chromatography and sequenced by N-terminal sequencing. The AtUSP gene amplified from an Arabidopsis leaf cDNA library was transformed to Escherichia coli to express the AtUSP protein. The recombinant protein...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Park SC,Jung YJ,Lee Y,Kim IR,Seol MA,Kim EJ,Jang MK,Lee JR

    更新日期:2017-05-13 00:00:00

  • BET inhibitor bromosporine enhances 5-FU effect in colorectal cancer cells.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Cheng X,Huang Z,Long D,Jin W

    更新日期:2020-01-22 00:00:00

  • Processing of the small GTP-binding protein SpYPT1p in Schizosaccharomyces pombe and in mammalian COS cells.

    abstract::SpYPT1p belongs to a family of ras-like GTP-binding proteins which is believed to be involved in the regulation of intracellular vesicular trafficking. We have analyzed the processing of this protein in its natural environment, the fission yeast Schizosaccharomyces pombe, and when expressed in transfected mammalian CO...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Giannakouros T,Newman CM,Armstrong J,Magee AI

    更新日期:1993-05-14 00:00:00

  • The N-terminal domain of tumor suppressor p53 is involved in the molecular interaction with the anti-apoptotic protein Bcl-Xl.

    abstract::Emerging evidences suggest that transcription-independent mechanism of p53 appears to make an important contribution to the overall p53-dependent apoptosis. Recently, it has been postulated that the DNA-binding domain of p53 can interact with Bcl-Xl, and subsequently the proposed molecular interaction has been shown b...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xu H,Tai J,Ye H,Kang CB,Yoon HS

    更新日期:2006-03-24 00:00:00

  • GTP-binding membrane proteins in activated and differentiating T cells.

    abstract::We have earlier reported changes in the GTP binding of several membrane proteins including Gs alpha and Gi alpha during thymic differentiation of T cells. Using an [alpha-32P]GTP-photoaffinity labeling technique we have studied the pattern of GTP binding proteins in activated and resting T lymphocytes and in T cells i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pessa-Morikawa T,Nordström T,Mustelin T,Andersson LC

    更新日期:1991-07-15 00:00:00

  • MiR-139-5p inhibits the proliferation of gastric cancer cells by targeting Regulation of Nuclear Pre-mRNA Domain Containing 1B.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wenquan L,Hongqing X,Yuhua L,Lili W,Wang Z,Ziwei Z,Chuang W,Aizhen C,Xiaosong W,Bo W,Lin C

    更新日期:2020-06-25 00:00:00

  • The Etv1 transcription factor activity-dependently downregulates a set of genes controlling cell growth and differentiation in maturing cerebellar granule cells.

    abstract::In the early postnatal period, cerebellar granule cells exhibit an activity-dependent downregulation of a set of immaturation genes involved in cell growth and migration and are shifted to establishment of a mature network formation. Through the use of a granule cell culture and both pharmacological and RNA interferen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Okazawa M,Abe H,Nakanishi S

    更新日期:2016-05-13 00:00:00

  • Promoter analysis of interleukin 19.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chen PJ,Wei CC,Wang C,Chen FW,Hsu YH,Chang MS

    更新日期:2006-06-09 00:00:00

  • Anti-glypican 3 antibodies cause ADCC against human hepatocellular carcinoma cells.

    abstract::Glypican 3 (GPC3), a GPI-anchored heparan sulfate proteoglycan, is expressed in the majority of hepatocellular carcinoma (HCC) tissues. Using MRL/lpr mice, we successfully generated a series of anti-GPC3 monoclonal antibodies (mAbs). GPC3 was partially cleaved between Arg358 and Ser359, generating a C-terminal 30-kDa ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nakano K,Orita T,Nezu J,Yoshino T,Ohizumi I,Sugimoto M,Furugaki K,Kinoshita Y,Ishiguro T,Hamakubo T,Kodama T,Aburatani H,Yamada-Okabe H,Tsuchiya M

    更新日期:2009-01-09 00:00:00

  • Knockdown of transcription factor forkhead box O3 (FOXO3) suppresses erythroid differentiation in human cells and zebrafish.

    abstract::Our previous study on the dynamic transcriptomes activated during human erythropoiesis suggested that transcription factor forkhead box O3 (FOXO3) possibly plays a role in erythroid differentiation. Functional studies in human cell line TF-1 indicated that FOXO3 knockdown repressed erythropoietin (EPO)-induced erythro...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang H,Li Y,Wang S,Zhang Q,Zheng J,Yang Y,Qi H,Qu H,Zhang Z,Liu F,Fang X

    更新日期:2015-05-15 00:00:00

  • Structure of prejunctional receptor binding analog of human neuropeptide Y dimer ANA-NPY.

    abstract::Human neuropeptide Y (NPY) analog ANA-NPY or [L17, Q19, A20, A23, L28, L31]NPY (13-36)-amide binds weakly to postjunctional receptors to raise blood pressure but binds tightly to prejunctional receptors to inhibit neurotransmitter release. ANA-NPY forms a well-conserved anti-parallel helical structure overlapping E23-...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Barden JA

    更新日期:1995-10-04 00:00:00

  • Crystal structure and biochemical characterization of malate dehydrogenase from Metallosphaera sedula.

    abstract::Metallosphaera sedula is a thermoacidophilic autotrophic archaeon and known to utilize the 3-hydroxypropionate/4-hydroxybutyrate cycle (3-HP/4-HB cycle) as a carbon fixation pathway. The 3-HP/4-HB cycle in M. sedula is associated with central metabolism, and malate dehydrogenase (MDH) is an enzyme involved in the cent...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lee D,Hong J,Kim KJ

    更新日期:2019-02-12 00:00:00

  • Inter-individual variation of several cytochrome P450 2D6 splice variants in human liver.

    abstract::To examine the possibility that inter-individual differences in splicing partially explain the observed differences in CYP2D6 activity, we amplified its full-length cDNA in 96 human liver RNA samples and discovered five splice variants: intron 5 retention, intron 6 retention, intron 5 and intron 6 double retention, ex...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Denson J,Wu Y,Yang W,Zhang J

    更新日期:2005-05-06 00:00:00

  • 9-Beta-D-arabinofuranosyladenine 5'-monophosphate (araAMP) is converted directly to its antivirally active 5'-triphosphate form by 5-phosphoribosyl-1-pyrophosphate (PRPP) synthetase.

    abstract::The antiherpetic agent 9-beta-D-arabinofuranosyladenine (araA) needs to be phosphorylated to its 5'-triphosphate to be effective as an inhibitor of herpes simplex virus replication. Adenosine kinase and deoxycytidine kinase are assumed to convert araA to its 5'-monophosphate. We now found that araAMP is converted to i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Balzarini J,De Clercq E

    更新日期:1990-12-31 00:00:00