当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Stable interdomain interaction within the cytoplasmic domain of CD45 increases enzyme stability.

Stable interdomain interaction within the cytoplasmic domain of CD45 increases enzyme stability.

Abstract：

:CD45 is a leukocyte-specific, two domain transmembrane tyrosine phosphatase. Co-purification of a recombinant protein containing the first phosphatase domain of CD45 (6His-D1) with a recombinant protein containing the second phosphatase domain (GST-D2) from E. coli indicated a stable interaction which resulted in increased stability of the active phosphatase domain present in 6His-D1. This interaction was not dependent on the acidic region unique to CD45 domain 2, but was affected by a destabilizing point mutation (Q1180G) in GST-D2. CD45 domain 2 enhanced phosphatase activity of the first domain in the full length cytoplasmic domain protein, whereas a chimeric protein with the SH2 domain of p56(lck) in place of the CD45 C-terminal region did not. Thus the C-terminal domain of CD45 associates with the N-terminal domain and this stabilizes the active phosphatase domain. A single destabilizing point mutation in the second domain is sufficient to attenuate this effect.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Felberg J,Johnson P

doi

10.1006/bbrc.2000.2623

subject

Has Abstract

pub_date

2000-05-10 00:00:00

pages

292-8

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(00)92623-8

journal_volume

271

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Derivation of cranial neural crest-like cells from human embryonic stem cells.

    abstract：:The neural crest is a transient population of multipotent progenitors contributing to a diverse array of tissues throughout the vertebrate embryo. Embryonic stem (ES) cells are able to form embryoid body and spontaneously differentiate to various lineages, following a reproducible temporal pattern of development that ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.09.032

    authors： Zhou Y,Snead ML

    更新日期：2008-11-21 00:00:00

  • Biotransformation of 1-nitropyrene and 2-nitrofluorene to novel metabolites, the corresponding formylamino compounds, in animal bodies.

    abstract：:The present study provides the first evidence that nitropolycyclic aromatic hydrocarbons such as 1-nitropyrene and 2-nitrofluorene are metabolized to the corresponding formylamino compounds in animal bodies. The study shows that the formation of such novel metabolites, 1-formylaminopyrene and 2-formylaminofluorene, is...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90284-1

    authors： Tatsumi K,Amano H

    更新日期：1987-01-30 00:00:00

  • The presence of free D-aspartic acid in rodents and man.

    abstract：:Free D-aspartic acid is present in appreciable quantities in the brain and other tissues of rodents and in human blood. In the newborn rat, the highest concentration of D-aspartic acid was found in cerebral hemispheres, where, at 164 nmol/g (8.4% of the total aspartic acid), the level of D-aspartic acid exceeds that o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80329-1

    authors： Dunlop DS,Neidle A,McHale D,Dunlop DM,Lajtha A

    更新日期：1986-11-26 00:00:00

  • Isoform-specific proteasomal degradation of Rbfox3 during chicken embryonic development.

    abstract：:Rbfox3, a neuron-specific RNA-binding protein, plays an important role in neuronal differentiation during development. An isoform Rbfox3-d31, which excludes the 93-nucleotide cassette exon within the RNA recognition motif of chicken Rbfox3, has been previously identified. However, the cellular functions of Rbfox3-d31 ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.07.057

    authors： Kim KK,Adelstein RS,Kawamoto S

    更新日期：2014-08-08 00:00:00

  • Critical importance of the de novo pyrimidine biosynthesis pathway for Trypanosoma cruzi growth in the mammalian host cell cytoplasm.

    abstract：:The intracellular parasitic protist Trypanosoma cruzi is the causative agent of Chagas disease in Latin America. In general, pyrimidine nucleotides are supplied by both de novo biosynthesis and salvage pathways. While epimastigotes-an insect form-possess both activities, amastigotes-an intracellular replicating form o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.12.073

    authors： Hashimoto M,Morales J,Fukai Y,Suzuki S,Takamiya S,Tsubouchi A,Inoue S,Inoue M,Kita K,Harada S,Tanaka A,Aoki T,Nara T

    更新日期：2012-01-20 00:00:00

  • alpha-Actinin links LPP, but not zyxin, to cadherin-based junctions.

    abstract：:The actin regulator VASP localizes to cell-cell junctions and has been implicated in cell-cell adhesion. VASP is recruited to sites of actin dynamics by interactions with proline rich FPPPPP motifs. Zyxin and its relative LPP use FPPPPP motifs to recruit VASP to specific cellular locations, thus directing changes in a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.04.018

    authors： Hansen MD,Beckerle MC

    更新日期：2008-06-20 00:00:00

  • Electron microscopic single particle analysis of a tetrameric RuvA/RuvB/Holliday junction DNA complex.

    abstract：:During the late stage of homologous recombination in prokaryotes, RuvA binds to the Holliday junction intermediate and executes branch migration in association with RuvB. The RuvA subunits form two distinct complexes with the Holliday junction: complex I with the single RuvA tetramer on one side of the four way juncti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.10.165

    authors： Mayanagi K,Fujiwara Y,Miyata T,Morikawa K

    更新日期：2008-01-11 00:00:00

  • Evidence for alpha-proton abstraction and carbanion formation involving a functional histidine residue in lentil seedling amine oxidase.

    abstract：:Lentil seedling amine oxidase catalyzes the oxidation of putrescine and in the presence of tetranitromethane gives rise to the formation of nitroform anion. The initial rate of substrate and enzyme-dependent nitroform production is linearly related to the functional active site content and is proportional to the tetra...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2401

    authors： Medda R,Padiglia A,Pedersen JZ,Floris G

    更新日期：1993-11-15 00:00:00

  • Immunoblot detection of pyridoxal phosphate binding proteins in liver and hepatoma cytosolic extracts.

    abstract：:A monoclonal antibody, highly selective for the 5'-phosphopyridoxyl group, can be used to detect cytosolic pyridoxal-5'-phosphate binding proteins by an immunoblot procedure. This technique, when applied to sodium borohydride-treated cytosolic extracts obtained from normal rat liver at various stages of development as...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91797-7

    authors： Kittler JM,Viceps-Madore D,Cidlowski JA,Thanassi JW

    更新日期：1983-04-15 00:00:00

  • OGT functions as a catalytic chaperone under heat stress response: a unique defense role of OGT in hyperthermia.

    abstract：:Protein O-GlcNAcylation is proceeded by O-linked GlcNAc transferase (OGT) in nucleocytoplasm and is involved in many biological processes although its physiological role is not clearly defined. To identify the functional significance of O-GlcNAcylation, we investigated heat stress effects on protein O-GlcNAcylation. H...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.08.023

    authors： Sohn KC,Lee KY,Park JE,Do SI

    更新日期：2004-09-24 00:00:00

  • CREB DNA binding activation by a 50-Hz magnetic field in HL60 cells is dependent on extra- and intracellular Ca(2+) but not PKA, PKC, ERK, or p38 MAPK.

    abstract：:To investigate the possible mechanism of gene transcription changes induced by magnetic field (MF), we examined the DNA binding behavior of the transcription factor cyclic-AMP responsive element binding protein (CREB) in HL60 cells after exposure to a 0.1mT 50-Hz extremely low frequency (ELF) sinusoidal MF by a gel sh...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02022-3

    authors： Zhou J,Yao G,Zhang J,Chang Z

    更新日期：2002-08-30 00:00:00

  • Cardiac-specific overexpression of SCN5A gene leads to shorter P wave duration and PR interval in transgenic mice.

    abstract：:The Cardiac sodium channel gene SCN5A plays a critical role in cardiac electrophysiology and its mutations, either gain- or loss-of-functions, are associated with lethal arrhythmias. In this study, we investigated the effect of overexpression of SCN5A on the cardiac phenotype in a transgenic mouse model (TG-WT L10). C...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.01.170

    authors： Zhang T,Yong SL,Tian XL,Wang QK

    更新日期：2007-04-06 00:00:00

  • TTX displacement of [H3]-nitrendipine binding in developing spinal cord neurons.

    abstract：:[3H] Nitrendipine binding was partially blocked by the presence of tetrodotoxin in developing spinal cord neurons. In young cultures, 1 micron tetrodotoxin displaced 29% and 26% of [3H] nitrendipine binding from the high and low affinity binding sites, respectively. In one month old cultures, tetrodotoxin had no effec...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91054-4

    authors： Litzinger MJ,Brenneman DE

    更新日期：1984-11-14 00:00:00

  • Molecular cloning and sequencing of the murine gastrin gene.

    abstract：:Gastrin is a peptide hormone important in acid regulation, growth of enterochromaffin cells of the oxyntic mucosa, and smooth muscle contractility. We isolated a genomic clone of gastrin from a SV-129 murine genomic library. The murine gastrin gene contains an open reading frame of 101 amino acids. The deduced amino a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2588

    authors： Koh TJ,Wang TC

    更新日期：1995-11-02 00:00:00

  • Estimation of pyruvate decarboxylation in perfused rat skeletal muscle.

    abstract：:By the determination of pyruvate dehydrogenase activity in tissue homogenates only limited information is gained on the actual metabolic flux. We therefore determined pyruvate decarboxylation in isolated rat hindlimbs non recirculating perfused with physiological (1-14C)pyruvate levels. On the basis of perfusate pyruv...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)90545-4

    authors： Schadewaldt P,Münch U,Prengel M,Staib W

    更新日期：1983-10-31 00:00:00

  • Tripartite motif-containing protein 3 plays a role of tumor inhibitor in cervical cancer.

    abstract：:Many studies have been reported that tripartite motif-containing (TRIM) proteins play important roles in various cellular processes and involved in many diseases. TRIM3, a member of TRIM family, has been proved that it plays important roles in various cancers. Nevertheless, its effects on cervical cancer reminded unkn...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.03.046

    authors： Song Y,Guo Q,Gao S,Hua K

    更新日期：2018-04-06 00:00:00

  • Decreased expression of glutaredoxin 1 is required for transforming growth factor-beta1-mediated epithelial-mesenchymal transition of EpRas mammary epithelial cells.

    abstract：:Transforming growth factor-beta (TGF-beta) is a cytokine important in inducing epithelial-mesenchymal transition (EMT), a crucial morphological event in a wide range of physiological and pathological cellular processes. In this study, we demonstrate that TGF-beta1 induces the EMT phenotype through decreasing the expre...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.12.009

    authors： Lee EK,Jeon WK,Chae MY,Hong HY,Lee YS,Kim JH,Kwon JY,Kim BC,Park SH

    更新日期：2010-01-01 00:00:00

  • Cloning and characterization of the 5'-flanking region of the human dopamine D4 receptor gene.

    abstract：:Dopamine D4 receptor (DRD4) has received attention in terms of pathogenesis of schizophrenia and association with human personalities. We isolated the human DRD4 gene containing the 5'-flanking region and determined its sequence. Analysis of the DRD4 transcripts by 5'-RACE (5'-rapid amplification of cDNA ends) reveale...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6770

    authors： Kamakura S,Iwaki A,Matsumoto M,Fukumaki Y

    更新日期：1997-06-18 00:00:00

  • Isolation and identification of C-type natriuretic peptide in human monocytic cell line, THP-1.

    abstract：:In a survey for unknown bioactive peptides in mammalian cell lines, we isolated peptides exhibiting a strong relaxant effect on chick rectum from a phorbol ester-supplemented culture medium of the human monocytic cell line, THP-1. The peptide was deduced to be a C-terminal 29-residue peptide derived from a human C-typ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)92257-x

    authors： Ishizaka Y,Kangawa K,Minamino N,Ishii K,Takano S,Eto T,Matsuo H

    更新日期：1992-12-15 00:00:00

  • Impaired adult hippocampal neurogenesis and its partial reversal by chronic treatment of fluoxetine in a mouse model of Angelman syndrome.

    abstract：:Angelman syndrome (AS) is a neurodevelopmental disorder characterized by severe cognitive and motor deficits, caused by the loss of function of maternally inherited Ube3a. Ube3a-maternal deficient mice (AS model mice) recapitulate many essential features of AS, but how the deficiency of Ube3a lead to such behavioural ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.07.103

    authors： Godavarthi SK,Dey P,Sharma A,Jana NR

    更新日期：2015-09-04 00:00:00

  • The homeodomain of Tinman mediates homo- and heterodimerization of NK proteins.

    abstract：:Cardiac development requires the action of transcription factors, which control the specification and differentiation of cardiac cell types. One of these factors, encoded by the homeobox gene tinman (tin), is essential for the specification of all cardiac cells in Drosophila. An increasing number of examples show that...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.06.090

    authors： Zaffran S,Frasch M

    更新日期：2005-08-26 00:00:00

  • Involvement of P2X4 receptor in P2X7 receptor-dependent cell death of mouse macrophages.

    abstract：:Interaction of P2X7 receptor with P2X4 receptor has recently been suggested, but it remains unclear whether P2X4 receptor is involved in P2X7 receptor-mediated events, such as cell death of macrophages induced by high concentrations of extracellular ATP. Here, we present evidence that P2X4 receptor does play a role in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.01.156

    authors： Kawano A,Tsukimoto M,Noguchi T,Hotta N,Harada H,Takenouchi T,Kitani H,Kojima S

    更新日期：2012-03-09 00:00:00

  • Scutellarin promotes in vitro angiogenesis in human umbilical vein endothelial cells.

    abstract：:Angiogenesis is critical to a wide range of physiological and pathological processes. Scutellarin, a major flavonoid of a Chinese herbal medicine Erigeron breviscapus (Vant.) Hand. Mazz. has been shown to offer beneficial effects on cardiovascular and cerebrovascular functions. However, scutellarin's effects on angiog...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.08.034

    authors： Gao ZX,Huang DY,Li HX,Zhang LN,Lv YH,Cui HD,Zheng JH

    更新日期：2010-09-10 00:00:00

  • Recovery of function and mass of endogenous beta-cells in streptozotocin-induced diabetic rats treated with islet transplantation.

    abstract：:Islet transplantation corrects chronic hyperglycemia by augmentation of insulin supply from the graft tissue, but the role of endogenous beta-cells after transplantation is not clear. In the present study, we examined endogenous beta-cell function after glucose homeostasis had been reestablished by islet graft in stre...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5563

    authors： Hamamoto Y,Tsuura Y,Fujimoto S,Nagata M,Takeda T,Mukai E,Fujita J,Yamada Y,Seino Y

    更新日期：2001-09-14 00:00:00

  • Identification of beta protein precursor in newborn rat brain.

    abstract：:We have identified by protein sequencing the precursor of beta protein in newborn rat brain. A rabbit antibody was raised against a synthetic peptide corresponding to the carboxyl-terminal 30 residues (666-695) of the putative beta-amyloid protein precursor (Kang et al. 1987). The antiserum recognized multiple bands a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(89)80144-5

    authors： Takio K,Hasegawa M,Titani K,Ihara Y

    更新日期：1989-05-15 00:00:00

  • Extracellular signal-regulated kinase (ERK) activity is required for TPA-mediated inhibition of drug-induced apoptosis.

    abstract：:Leukemia cells respond to toxic stimuli by undergoing a form of programmed cell death known as apoptosis. However, the signaling events responsible for the execution of this form of death are poorly understood. Mitogen-activated protein kinase (MAPK) signaling cascades are involved in the cellular response to extracel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8410

    authors： Stadheim TA,Kucera GL

    更新日期：1998-04-07 00:00:00

  • Characterization of the exchange reactions of rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase.

    abstract：:The Fru-6-P/Fru-2,6-P2 exchange reaction of rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase was almost entirely dependent on the presence of Pi. This exchange was not due to a reversal of the bisphosphatase nor to trace amounts of adenine nucleotide in the enzyme. Exchange activity was maximal at pH 7, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90293-6

    authors： El-Maghrabi MR,Pate TM,Pilkis SJ

    更新日期：1984-09-17 00:00:00

  • IL-2-mediated upregulation of uPA and uPAR in natural killer cells.

    abstract：:Urokinase plasminogen activator (uPA) and its receptor uPAR play a major role in immune cell-mediated, including natural killer (NK) cell-mediated, degradation of extracellular matrices. Herein, we investigate the effects of IL-2 on NK cell uPA and uPAR. RNA and protein analyses showed upregulation of uPA and uPAR fol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6627

    authors： Al-Atrash G,Shetty S,Idell S,Xue Y,Kitson RP,Halady PK,Goldfarb RH

    更新日期：2002-03-22 00:00:00

  • Effects of replacement of tryptophan-140 by phenylalanine or glycine on the function of Escherichia coli aspartate aminotransferase.

    abstract：:Trp140 of E. coli aspartate aminotransferase has been converted to Phe or Gly by site-directed mutagenesis. As compared to the wild-type enzyme, either of the mutant enzymes showed 10- to 100-fold increase in Km's for natural dicarboxylic substrates, but did not show appreciable changes in Km's for aromatic substrates...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)92037-z

    authors： Hayashi H,Inoue Y,Kuramitsu S,Morino Y,Kagamiyama H

    更新日期：1990-03-16 00:00:00

  • Activation of the p21(Waf1/Cip1) promoter by the ets oncogene family transcription factor E1AF.

    abstract：:p21(Waf1/Cip1) is one of the key regulatory proteins in cell cycle, terminal differentiation, and apoptosis. Its promoter was shown to be transactivated by the wild-type p53 protein as well as in a p53-independent manner. In this report, we demonstrate that E1AF, an ets-related transcription factor, activates the huma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6909

    authors： Funaoka K,Shindoh M,Yoshida K,Hanzawa M,Hida K,Nishikata S,Totsuka Y,Fujinaga K

    更新日期：1997-07-09 00:00:00