当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Electron microscopic single particle analysis of a tetrameric RuvA/RuvB/Holliday junction DNA complex.

Electron microscopic single particle analysis of a tetrameric RuvA/RuvB/Holliday junction DNA complex.

Abstract：

:During the late stage of homologous recombination in prokaryotes, RuvA binds to the Holliday junction intermediate and executes branch migration in association with RuvB. The RuvA subunits form two distinct complexes with the Holliday junction: complex I with the single RuvA tetramer on one side of the four way junction DNA, and complex II with two tetramers on both sides. To investigate the functional roles of complexes I and II, we mutated two residues of RuvA (L125D and E126K) to prevent octamer formation. An electron microscopic analysis indicated that the mutant RuvA/RuvB/Holliday junction DNA complex formed the characteristic tripartite structure, with only one RuvA tetramer bound to one side of the Holliday junction, demonstrating the unexpected stability of this complex. The novel bent images of the complex revealed an intriguing morphological similarity to the structure of SV40 large T antigen, which belongs to the same AAA+ family as RuvB.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Mayanagi K,Fujiwara Y,Miyata T,Morikawa K

doi

10.1016/j.bbrc.2007.10.165

subject

Has Abstract

pub_date

2008-01-11 00:00:00

pages

273-8

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)02341-8

journal_volume

365

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Increased antiviral and opsonic activity of a highly multimerized collectin chimera.

    abstract：:Altering the carbohydrate binding properties of surfactant protein D (SP-D) [e.g., by replacing its carbohydrate recognition domain (CRD) with that of either mannose binding lectin (MBL) or conglutinin] can increase its activity against influenza A virus (IAV). The current study demonstrates that the degree of multime...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5373

    authors： White MR,Crouch E,Chang D,Hartshorn KL

    更新日期：2001-08-10 00:00:00

  • Lithium inhibits glycogen synthase kinase-3 by competition for magnesium.

    abstract：:The mechanism by which lithium (Li(+)) inhibits the protein kinase glycogen synthase kinase-3 (GSK-3) is unknown. Here, we demonstrate that Li(+) is a competitive inhibitor of GSK-3 with respect to magnesium (Mg(2+)), but not to substrate or ATP. This mode of inhibition is conserved between mammalian and Dictyostelium...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.4169

    authors： Ryves WJ,Harwood AJ

    更新日期：2001-01-26 00:00:00

  • Acid-sensing by the T cell death-associated gene 8 (TDAG8) receptor cloned from rat brain.

    abstract：:The T cell death-associated gene 8 (TDAG8) is a pH-sensing GPCR with a reported immune-specific expression profile. Here, we demonstrate pH-induced activation of TDAG8 receptor cloned from rodent brain (rTDAG8). Cloned rTDAG8 transcript showed 88-95% homology with human and mouse transcripts of lymphoid origin. RT-PCR...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.05.133

    authors： McGuire J,Herman JP,Ghosal S,Eaton K,Sallee FR,Sah R

    更新日期：2009-08-28 00:00:00

  • Generation of the replication-competent human immunodeficiency virus type 1 which expresses a jellyfish green fluorescent protein.

    abstract：:The gene encoding a red-shifted green fluorescent protein variant (EGFP) was introduced into a human immunodeficiency virus type 1 (HIV-1) molecular clone by replacing the nef gene. The EGFP-expressing HIV-1 replicated efficiently in established human T cells. The expression of EGFP in the virus-infected cells was con...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6304

    authors： Lee AH,Han JM,Sung YC

    更新日期：1997-04-07 00:00:00

  • Torsion of the central pair microtubules in eukaryotic flagella due to bending-driven lateral buckling.

    abstract：:Inspired by recent interest in torsion of the central pair microtubules in eukaryotic flagella, a novel thin-walled elastic beam model is suggested to study critical condition under which uniform bending of a flagellum will cause lateral/torsional buckling of the central pair. The model is directed to the central pair...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.10.019

    authors： Li C,Ru CQ,Mioduchowski A

    更新日期：2006-12-08 00:00:00

  • Resistance to tetracycline, a hydrophilic antibiotic, is mediated by P-glycoprotein in human multidrug-resistant cells.

    abstract：:Two multidrug-resistant human leukemic CCRF-CEM sublines (CEM/VCR R and CEM/VLB100) were significantly more resistant to tetracycline, a hydrophilic antibiotic, than parental cells (P < 0.001). Verapamil and cyclosporin A completely reversed tetracycline resistance in CEM/VCR R cells, which also accumulated and retain...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1013

    authors： Kavallaris M,Madafiglio J,Norris MD,Haber M

    更新日期：1993-01-15 00:00:00

  • Disassembly of the post-termination complex and reduction of translational error by ribosome recycling factor (RRF)-A possible new target for antibacterial agents.

    abstract：:In this paper, we briefly review RRF (ribosome recycling factor, previously called ribosome releasing factor) (for recent reviews covering historical background see (1, 2)). ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1006/bbrc.1998.9168

    authors： Kaji A,Teyssier E,Hirokawa G

    更新日期：1998-09-08 00:00:00

  • Hairpin antisense oligonucleotides containing 2'-methoxynucleosides with base-pairing in the stem region at the 3'-end: penetration, localization, and Anti-HIV activity.

    abstract：:Hairpin antisense oligodeoxyribonucleotides containing 2'-methoxynucleosides were more active in the micromolar concentration range than linear and DNA hairpin phosphorothioate oligonucleotides with the same sequence. Furthermore, the abilities of hairpin antisense and random hairpin phosphorothioate oligonucleotides ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1707

    authors： Kuwasaki T,Hosono K,Takai K,Ushijima K,Nakashima H,Saito T,Yamamoto N,Takaku H

    更新日期：1996-11-12 00:00:00

  • Electron transfer between liposomal cytochrome c1 and cytochrome c: catalytic implications of electrostatic potentials.

    abstract：:Kinetics measurements of the electron transfer between ferricytochrome c and liposomal ferrocytochrome c1 (with and without the hinge protein) were performed. The observed rate constants(kobs) of electron transfer between liposomal ferrocytochrome c1 and ferricytochrome c at different ionic strengths were measured in ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92132-3

    authors： Kim CH,King TE,Balny C

    更新日期：1989-08-30 00:00:00

  • Allele specific gain-of-function activity of p53 mutants in lung cancer cells.

    abstract：:p53 mutations are mostly single amino acid changes resulting in expression of a stable mutant protein with "gain of function" (GOF) activity having a dominant oncogenic role rather than simple loss of function of wild-type p53. Knock-down of mutant p53 in human lung cancer cell lines with different endogenous p53 muta...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.09.029

    authors： Vaughan CA,Frum R,Pearsall I,Singh S,Windle B,Yeudall A,Deb SP,Deb S

    更新日期：2012-11-09 00:00:00

  • Alteration of the subcellular localization of hepatitis B virus core protein by large but not small surface proteins.

    abstract：:By performing immunofluorescence staining assays in confluent COS-7 cells, hepatitis B virus core, small surface and large surface proteins were found to localize largely in the nucleus, diffusely in the cytoplasm, and accumulatively in a perinuclear area, respectively. When two proteins were co-expressed, the core pr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2330

    authors： Yeh CT,Ou JH,Chu CM,Liaw YF

    更新日期：1994-09-15 00:00:00

  • Inhibition of AMP-activated protein kinase suppresses IL-2 expression through down-regulation of NF-AT and AP-1 activation in Jurkat T cells.

    abstract：:AMP-activated protein kinase (AMPK) is a key regulator of energy homeostasis and its activation during T cell receptor stimulation has recently been reported. In this study, we examined the role of AMPK in interleukin (IL)-2 production in T cells. Inhibition of AMPK by compound C, a specific inhibitor of AMPK or small...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.10.138

    authors： Jhun BS,Lee JY,Oh YT,Lee JH,Choe W,Baik HH,Kim SS,Yoon KS,Ha J,Kang I

    更新日期：2006-12-29 00:00:00

  • Covalent circularization of exogenous RNA during incubation with a wheat embryo cell extract.

    abstract：:Cell extracts from wheat embryos have been widely used for mRNA-directed protein production. Here, we found that a significant fraction of exogenous linear RNAs are circularized in a wheat embryo extract. The circularization was seen only in uncapped RNAs. The amount of the circular species reached around 1% of the in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.07.011

    authors： Makino S,Sawasaki T,Tozawa Y,Endo Y,Takai K

    更新日期：2006-09-08 00:00:00

  • Hepatitis C NS3 protease: restoration of NS4A cofactor activity by N-biotinylation of mutated NS4A using synthetic peptides.

    abstract：:The NS3 serine protase of Hepatitis C virus (HCV) requires NS4A protein as a cofactor for efficient cleavage at four sites in the nonstructural region. The cofactor activity has been mapped to the central hydrophobic region (aa 22-34) of this 54-amino-acid NS4A protein, and site-directed mutagenesis has identified alt...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1898

    authors： Butkiewicz NJ,Yao N,Wright-Minogue J,Zhang R,Ramanathan L,Lau JY,Hong Z,Dasmahapatra B

    更新日期：2000-01-07 00:00:00

  • Anti-ischemia/reperfusion of C1 inhibitor in myocardial cell injury via regulation of local myocardial C3 activity.

    abstract：:C3 is common to all pathways of complement activation augmenting ischemia/reperfusion (I/R)-induced myocardial injury and cardiac dysfunction. Complement inhibition with the complement regulatory protein, C1 inhibitor (C1INH), obviously exerts cardioprotective effects. Here, we examine whether C1INH regulates C3 activ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.09.023

    authors： Fu J,Lin G,Zeng B,Wu Z,Wu Y,Chu H,Qin G,Liang G,Li J,Gan X,Yu X,Li C,Liu D

    更新日期：2006-11-10 00:00:00

  • A distinct form of tau is selectively incorporated into Alzheimer's paired helical filaments.

    abstract：:Tau, a microtubule-associated phosphoprotein, was identified as a definite component of paired helical filaments which progressively accumulate in Alzheimer's disease brain. To learn more about tau in the aged brain, we have isolated and sequenced a cDNA clone encoding tau from a cDNA library of an aged human brain. T...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92240-7

    authors： Mori H,Hamada Y,Kawaguchi M,Honda T,Kondo J,Ihara Y

    更新日期：1989-03-31 00:00:00

  • Two cis-acting regulatory sequences in the peroxisome proliferator-responsive enhancer region of rat acyl-CoA oxidase gene.

    abstract：:To clarify the mechanism of transcriptional induction of the rat liver acyl-CoA oxidase gene by hypolipidemic agents, we searched for cis-acting regulatory sequences in the 5' upstream region of the gene by transfection studies. We found that the sequence between -639 and -472 acts as a peroxisome proliferator-respons...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91645-s

    authors： Osumi T,Wen JK,Hashimoto T

    更新日期：1991-03-29 00:00:00

  • Co-culture of human CD34+ cells with mesenchymal stem cells increases the survival of CD34+ cells against the 5-aza-deoxycytidine- or trichostatin A-induced cell death.

    abstract：:It has been suggested that epigenetic regulation plays an important role in maintaining the stemness and lineage differentiation of hematopoietic stem cells (HSCs), 5-aza-deoxycytidine (aza-D) and Trichostatin A (TSA) being candidate additives for HSC ex vivo expansion. Although they have potent activity to maintain t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.02.077

    authors： Koh SH,Choi HS,Park ES,Kang HJ,Ahn HS,Shin HY

    更新日期：2005-04-15 00:00:00

  • Cloning and expression of a rat cytochrome P-450 11 beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant.

    abstract：:A variant cytochrome P450 11 beta-hydroxylase/aldosterone synthase cDNA (CYP11B2) clone, pALDO-4, was isolated and sequenced from a rat zona glomerulosa cDNA library. This clone contains a 1521 bp open reading frame coding for a 506 amino acid residue protein which has a 30 amino acid extension peptide which is 6 resi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1792

    authors： Zhou M,Gomez-Sanchez CE

    更新日期：1993-07-15 00:00:00

  • Inter-individual variation of several cytochrome P450 2D6 splice variants in human liver.

    abstract：:To examine the possibility that inter-individual differences in splicing partially explain the observed differences in CYP2D6 activity, we amplified its full-length cDNA in 96 human liver RNA samples and discovered five splice variants: intron 5 retention, intron 6 retention, intron 5 and intron 6 double retention, ex...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.010

    authors： Denson J,Wu Y,Yang W,Zhang J

    更新日期：2005-05-06 00:00:00

  • Expression of a long variant of CRACR2A that belongs to the Rab GTPase protein family in endothelial cells.

    abstract：:CRACR2A protein is described in T cells as an EF-hand-containing modulator of calcium-release-activated calcium (CRAC) channels. Here we sought relevance to calcium entry of endothelial cells. Unexpectedly, short interfering RNA designed to deplete CRACR2A had no effect on CRAC channels in endothelial cells but reduce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.11.095

    authors： Wilson LA,McKeown L,Tumova S,Li J,Beech DJ

    更新日期：2015-01-02 00:00:00

  • 1,25-Dihydroxyvitamin D3 enhancement of concanavalin-A induced bovine lymphocyte proliferation: requirement of monocytes.

    abstract：:The effect of 1,25-(OH)2D3 on Concanavalin-A (ConA) mediated bovine lymphocyte proliferation was dependent on the magnitude of the proliferative response. Lymphocytes from cows with high inherent proliferative responses (134,904 +/- 15,488 cpm/well) were unaffected by 1,25-(OH)2D3; whereas, lymphocytes from cows with ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80072-x

    authors： Hustmyer FG,Nonnecke BJ,Beitz DC,Horst RL,Reinhardt TA

    更新日期：1988-04-29 00:00:00

  • Chemokine changes during oral wound healing.

    abstract：:The oral mucosa is susceptible to tissue injury from many causes, including infection, autoimmune disorders, surgical and accidental trauma, and gingival and periodontal inflammation; however, little is known about the events that influence wound healing in the mouth. Recent studies in non-oral tissues have implicated...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.09.056

    authors： McGrory K,Flaitz CM,Klein JR

    更新日期：2004-11-05 00:00:00

  • Lipoprotein lipase: role of intramolecular disulfide bonds in enzyme catalysis.

    abstract：:Lipoprotein lipase (LPL) catalyzes the hydrolysis of the triacylglycerol component of triacylglycerol-rich lipoproteins. There are 4 cysteine pairs that are completely conserved among LPLs of all species known. We examined the functional importance of each of the cysteine pairs in enzyme catalysis by examining LPLs pr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1037

    authors： Lo JY,Smith LC,Chan L

    更新日期：1995-01-05 00:00:00

  • Abnormal physical architecture of the lipophilic domains of human sperm membrane in oligospermia: a logical cause for low fertility profiles.

    abstract：:Three different domains of human sperm membrane, viz., the hydrophobic domains, the aqueous compartments and the surface proteins were probed to evaluate the molecular dynamics in these microenvironments in normal as well as oligospermic cases. Decreased rotational motion of the stearic spin-labels, designated rigid l...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1037

    authors： Sinha S,Kumar GP,Laloraya M

    更新日期：1994-01-14 00:00:00

  • Sepsis-induced muscle proteolysis is prevented by a proteasome inhibitor in vivo.

    abstract：:Sepsis-induced muscle proteolysis mainly reflects ubiquitin-proteasome-dependent protein degradation. The effect of in vivo administration of a proteasome inhibitor on muscle protein breakdown during sepsis is not known. We treated rats with the proteasome inhibitor N-benzyloxycarbonyl-Ile-Glu-(O-t-butyl)-Ala-leucinal...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2398

    authors： Fischer D,Gang G,Pritts T,Hasselgren PO

    更新日期：2000-04-02 00:00:00

  • Down-regulation of TRAF4 targeting RSK4 inhibits proliferation, invasion and metastasis in breast cancer xenografts.

    abstract：:Ribosomal S6 protein kinase 4 (RSK4) was known as a novel tumor suppressor gene, and the tumor necrosis factor receptor-associated factor 4 (TRAF4) was linked to carcinogenesis. The purpose of this study is to further investigate the effect of the TRAF4 gene on cell proliferation, invasion and metastasis in vivo and e...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.04.164

    authors： Zhu L,Zhang S,Huan X,Mei Y,Yang H

    更新日期：2018-06-07 00:00:00

  • Abnormal activation of K+ channels underlies relaxation to bacterial lipopolysaccharide in rat aorta.

    abstract：:We have examined the role of K+ channels in mediating vasorelaxation produced by bacterial lipopolysaccharide (LPS) in endothelial-denuded strips of rat aorta precontracted with phenylephrine (1 microM). Salmonella typhosa LPS (0.1 microgram/ml) caused significant relaxation of tension which peaked at approximately 4h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1005

    authors： Hall S,Turcato S,Clapp L

    更新日期：1996-07-05 00:00:00

  • Pyrophosphate functions as phosphoryl donor with UDP-glucose-treated mammalian phosphofructokinase.

    abstract：:Phosphofructokinase of rabbit muscle, which is specific for nucleoside triphosphates such as ATP, dissociated and gained the capability to utilize pyrophosphate as phosphoryl donor following incubation with UDP-glucose. The pyrophosphate- and ATP-linked activities of UDP-glucose-treated muscle phosphofructokinase were...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90754-x

    authors： Wong JH,Balogh A,Buchanan BB

    更新日期：1984-06-29 00:00:00

  • Inhibition of B7-H3 reverses oxaliplatin resistance in human colorectal cancer cells.

    abstract：:B7-H3, an immunoregulatory protein, has been found highly expressed in several cancer types, and involved in cancer cell migration and invasion. Here, we investigated the role of B7-H3 in oxaliplatin resistance in colorectal cancer (CRC) cells. Transient silencing of B7-H3 enhanced oxaliplatin sensitivity by increasin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.07.001

    authors： Zhang P,Chen Z,Ning K,Jin J,Han X

    更新日期：2017-08-26 00:00:00