当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Electron microscopic single particle analysis of a tetrameric RuvA/RuvB/Holliday junction DNA complex.

Electron microscopic single particle analysis of a tetrameric RuvA/RuvB/Holliday junction DNA complex.

Abstract：

:During the late stage of homologous recombination in prokaryotes, RuvA binds to the Holliday junction intermediate and executes branch migration in association with RuvB. The RuvA subunits form two distinct complexes with the Holliday junction: complex I with the single RuvA tetramer on one side of the four way junction DNA, and complex II with two tetramers on both sides. To investigate the functional roles of complexes I and II, we mutated two residues of RuvA (L125D and E126K) to prevent octamer formation. An electron microscopic analysis indicated that the mutant RuvA/RuvB/Holliday junction DNA complex formed the characteristic tripartite structure, with only one RuvA tetramer bound to one side of the Holliday junction, demonstrating the unexpected stability of this complex. The novel bent images of the complex revealed an intriguing morphological similarity to the structure of SV40 large T antigen, which belongs to the same AAA+ family as RuvB.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Mayanagi K,Fujiwara Y,Miyata T,Morikawa K

doi

10.1016/j.bbrc.2007.10.165

subject

Has Abstract

pub_date

2008-01-11 00:00:00

pages

273-8

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)02341-8

journal_volume

365

pub_type

杂志文章

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