Expression of recombinant rat interleukin-13 (IL-13) and generation of a neutralizing rat IL-13 antiserum.

abstract：:Prion protein (PrPc) is expressed in many tissues, both in human and animals. The scrapie isoform of PrPc has been shown to cause neurodegeneration. In other studies it has been demonstrated that overexpression of the PrP gene can result in nonneuronal tissue degradation. Little is known, however, about the normal fun...

journal_title：Biochemical and biophysical research communications

authors： Kniazeva M,Orman R,Terranova VP

更新日期：1997-05-08 00:00:00

abstract：:Bacterial endotoxins or lipopolysaccharides (LPS), cell wall components of gram negative bacteria, are involved in septic shock. While the carbohydrate structure of LPS have been studied in the past, little is known about the macromolecular structure and formation of LPS fragments in blood. It is believed that amphiph...

journal_title：Biochemical and biophysical research communications

authors： Aurell CA,Wistrom AO

更新日期：1998-12-09 00:00:00

abstract：:In order to study the relationship between insulin like growth factor-II (IGF-II) and interleukin-8 (IL-8) that are upregulated in psoriasis, we monitored IL-8 expression in IGF-II-treated human keratinocytes and explored the signaling pathways of IL-8 expression by IGF-II. IGF-II increased the IL-8 mRNA and protein l...

journal_title：Biochemical and biophysical research communications

authors： Kim HJ,Byun SJ,Kim TY

更新日期：2004-04-23 00:00:00

abstract：:Alcohol dehydrogenase (ADH; EC 1.1.1.1) and aldehyde dehydrogenase (ALDH; EC 1.2.1.3.) are important enzymes involved in the biotransformation of both alcohols and aldehydes. Today, six classes of ADH and twelve classes of ALDH have been defined in mammals. Here we report the detection and localisation of three classe...

journal_title：Biochemical and biophysical research communications

authors： Cheung C,Smith CK,Hoog JO,Hotchkiss SA

更新日期：1999-07-22 00:00:00

abstract：:Muscarinic receptor extracted from porcine atria in digitonin-cholate copurified with Galpha(o), Galpha(i1-3), and caveolins. The presence of complexes was confirmed by coimmunoprecipitation of the receptor, alpha-subunits, and caveolins in various combinations. Homooligomers of alpha(i2) were detected on Western blot...

journal_title：Biochemical and biophysical research communications

authors： Ma AW,Pawagi AB,Wells JW

更新日期：2008-09-12 00:00:00

abstract：:Kallikrein 7 (hK7), a chymostatin-like serine protease, is overexpressed in pancreatic adenocarcinomas as well as other human cancers. Although it has been demonstrated to participate in normal desquamation by facilitating cell shedding at the skin surface, its role in human malignancies remains unclear. To investigat...

journal_title：Biochemical and biophysical research communications

authors： Ramani VC,Haun RS

更新日期：2008-05-16 00:00:00

abstract：:Here we present a simple and highly reproducible method which allows the study of the effects of a single gene knockdown in an organotypic skin model. Human keratinocytes (KC) were transfected with backbone-modified short interfering RNAs (siRNAs) specific for vascular endothelial growth factor (VEGF) and matriptase-1...

journal_title：Biochemical and biophysical research communications

authors： Mildner M,Ballaun C,Stichenwirth M,Bauer R,Gmeiner R,Buchberger M,Mlitz V,Tschachler E

更新日期：2006-09-15 00:00:00

abstract：:Mesenchymal Stem Cells are known to engraft and integrate into the architecture of colorectal tumours, with little known regarding their fate following engraftment. This study aimed to investigate mediators of Mesenchymal Stem Cell (MSC) and colon cancer cell (CCC) interactions. Mesenchymal Stem Cells and colon cancer...

journal_title：Biochemical and biophysical research communications

authors： Hogan NM,Joyce MR,Murphy JM,Barry FP,O'Brien T,Kerin MJ,Dwyer RM

更新日期：2013-06-14 00:00:00

abstract：:This article investigated the effects of pramipexole on myocardial ischemia reperfusion (I/R) injury and its underlying mechanisms. We utilized an in vivo mouse model of myocardial I/R injury and an in vitro H9c2 cell model of hypoxia/reoxygenation (H/R) injury. Pramipexole pretreatment in male C57BL/6 mice significan...

journal_title：Biochemical and biophysical research communications

authors： Mo Y,Tang L,Ma Y,Wu S

更新日期：2016-05-13 00:00:00

abstract：:Human bone marrow mesenchymal stem cells (hMSCs) are a promising source for clinical stem cell transplantation. However, telomere regulation mechanisms, as one of the possible major mechanisms by which hMSCs sustain their stem cell characteristics, remain unknown. We isolated hMSCs by plastic adhesion and characterize...

journal_title：Biochemical and biophysical research communications

authors： Zhao YM,Li JY,Lan JP,Lai XY,Luo Y,Sun J,Yu J,Zhu YY,Zeng FF,Zhou Q,Huang H

更新日期：2008-05-16 00:00:00

abstract：:MAEBL is an erythrocyte binding protein located in the rhoptries and on the surface of mature merozoites, being expressed at the beginning of schizogony. The structure of MAEBL originally isolated from rodent malaria parasites suggested a molecule likely to be involved in invasion. We thus became interested in identif...

journal_title：Biochemical and biophysical research communications

authors： Ocampo M,Curtidor H,Vera R,Valbuena JJ,Rodríguez LE,Puentes A,López R,García JE,Tovar D,Pacheco P,Navarro MA,Patarroyo ME

更新日期：2004-03-05 00:00:00

abstract：:Since our experimental results suggest that UT-B1 functions as active water transporter against osmotic gradient in C6 glial cells, we report here for the first time the evidence for the active water transport. Exposure of C6 cells to a hyperosmotic solution containing glycerol or sucrose produced cell shrinkage due t...

journal_title：Biochemical and biophysical research communications

authors： Ogami A,Miyazaki H,Niisato N,Sugimoto T,Marunaka Y

更新日期：2006-12-22 00:00:00

abstract：:Bloom syndrome (BS) is a rare genetic disorder characterized by small body size, sun sensitivity, immunodeficiency and a high predisposition to various types of cancer. BLM was identified as the causative gene for BS, and BLM protein is homologous to DNA helicase. There are two putative nuclear localization signals (N...

journal_title：Biochemical and biophysical research communications

authors： Kaneko H,Orii KO,Matsui E,Shimozawa N,Fukao T,Matsumoto T,Shimamoto A,Furuichi Y,Hayakawa S,Kasahara K,Kondo N

更新日期：1997-11-17 00:00:00

abstract：:Previously we reported that the activity of protein kinase FA/glycogen synthase kinase-3alpha (kinase FA/GSK-3alpha) can be detected in several brain membrane fractions. In this report, we examined whether kinase FA/GSK-3alpha can directly interact with membrane phospholipids by using anti-kinase FA/GSK-3alpha antibod...

journal_title：Biochemical and biophysical research communications

authors： Yu JS,Chan WH,Yang SD

更新日期：1997-08-18 00:00:00

abstract：:Centrin is an EF-hand calcium-binding protein found in microtubule organizing centers of organisms ranging from algae and yeast to man. Phosphorylation in the centrin C-terminal domain occurs in mitosis and is associated with alterations in contractile fibers. To obtain insight into the structural basis for the functi...

journal_title：Biochemical and biophysical research communications

authors： Meyn SM,Seda C,Campbell M,Weiss KL,Hu H,Pastrana-Rios B,Chazin WJ

更新日期：2006-03-31 00:00:00

abstract：:The transcription factor hypoxia-inducible factor-1 (HIF-1) functions as a master regulator of hypoxic response by inducing the transcription of various genes responsible for cellular adaptation to hypoxia. In this study, we investigated the effects of protein inhibitor of activated STAT3 (PIAS3), a small ubiquitin-re...

journal_title：Biochemical and biophysical research communications

authors： Nakagawa K,Kohara T,Uehata Y,Miyakawa Y,Sato-Ueshima M,Okubo N,Asaka M,Takeda H,Kobayashi M

更新日期：2016-01-15 00:00:00

abstract：:We have recently demonstrated that exposure of MCF7 cells to antiestrogens in vitro results in both accumulation of IGF-BP3 in media and reduced mitogenic responsivity to IGFs (Cancer Res. 53:5193-5198). We show here that MCF7 cell proliferation in steroid-stripped, serum containing media is significantly attenuated b...

journal_title：Biochemical and biophysical research communications

authors： Pratt SE,Pollak MN

更新日期：1994-01-14 00:00:00

abstract：:Free energy changes for ATP synthesis (delta GP) and 2e(-)-transfer across Site 3 (delta GR) were determined during oxidative phosphorylation by rat liver mitochondria. At static head, -delta GR/delta GP ranged narrowly between 1.55 and 1.59 with five different respiratory substrates. Thus, an ATP/2e- of 1 1/2 at Site...

journal_title：Biochemical and biophysical research communications

authors： Lemasters JJ,Fleishman KE

更新日期：1987-01-15 00:00:00

abstract：:Pentamannose 6-phosphate/trilysine substituted aprotinin (PMP-lys-aprotinin) and insulin like growth factor II (IGF II) were used as affinity ligands for the mannose 6-phosphate (M6P) and IGF II binding sites of the M6P/IGF II receptor. Both ligands were cross linked to intact receptor and tryptic fragments of the rec...

journal_title：Biochemical and biophysical research communications

authors： Waheed A,Braulke T,Junghans U,von Figura K

更新日期：1988-05-16 00:00:00

abstract：:The receptor for calcitonin gene-related peptide (CGRP) has been the target for the development of novel small molecule antagonists for the treatment of migraine. Two such antagonists, BIBN4096BS and MK-0974, have shown great promise in clinical trials and hence a deeper understanding of the mechanism of their interac...

journal_title：Biochemical and biophysical research communications

authors： Miller PS,Barwell J,Poyner DR,Wigglesworth MJ,Garland SL,Donnelly D

更新日期：2010-01-01 00:00:00

abstract：:Helicobacter pylori is a pathogenic flagellated bacterium that infects the gastroduodenal mucosa and causes peptic ulcers in humans. FliD caps the distal end of the flagellar filament and is essential in filament growth. Moreover, FliD has been studied to diagnose and prevent H. pylori infection. Here, we report struc...

journal_title：Biochemical and biophysical research communications

authors： Cho SY,Song WS,Oh HB,Kim HU,Jung HS,Yoon SI

更新日期：2019-06-18 00:00:00

abstract：:In RBL-2H3 rat basophilic leukemia cells, Fc epsilon R1 crosslinking by multivalent antigen stimulates phosphatidylinositol (PI) turnover and Ca2+ influx and causes functional responses that include secretion, membrane ruffling and actin polymerization. Here, we show that the tyrosine kinase inhibitor, genistein, inhi...

journal_title：Biochemical and biophysical research communications

authors： Deanin GG,Martinez AM,Pfeiffer JR,Gardner ME,Oliver JM

更新日期：1991-08-30 00:00:00

abstract：:The (1)H NMR spectrum of the perchloric acid extract of carp seminal plasma was heavily congested. It is demonstrated that proton-detected C,H chemical shift correlation spectroscopy (HSQC, HSQC-TOCSY) allows an unequivocal identification of proline, glutamate, taurine, and methionine sulfoxide, although several key p...

journal_title：Biochemical and biophysical research communications

authors： Kalic M,Lauterwein J,Büsselmann G,Kamp G

更新日期：2000-03-05 00:00:00

abstract：:In cells, there exists a delicate balance between accumulation of charged metal cations and abundant anionic complexes such as phosphate. When phosphate metabolism is disrupted, cell-wide spread disturbances in metal homeostasis may ensue. The best example is a yeast pho80 mutant that hyperaccumulates phosphate and as...

journal_title：Biochemical and biophysical research communications

authors： Rosenfeld L,Culotta VC

更新日期：2012-02-10 00:00:00

abstract：:Wnt-1, the vertebrate counterpart of the Drosophila wingless gene, plays an important role in the early morphogenesis of neural tissues. In this report, we have shown that overexpression of Wnt-1 can direct embryonic carcinoma P19 cells to differentiate into neuron-like cells in the absence of retinoic acid. Immunocyt...

journal_title：Biochemical and biophysical research communications

authors： Tang K,Yang J,Gao X,Wang C,Liu L,Kitani H,Atsumi T,Jing N

更新日期：2002-04-26 00:00:00

abstract：:We have previously shown that silkworm hemolymph exhibits anti-apoptotic activity against baculovirus-induced Sf9 cell apoptosis. In this study, using various chemical inducers, such as actinomycin D, camptothecin, and staurosporine, we found that silkworm hemolymph inhibits insect cell apoptosis induced not only by b...

journal_title：Biochemical and biophysical research communications

authors： Rhee WJ,Kim EJ,Park TH

更新日期：2002-07-26 00:00:00

abstract：OBJECTIVE:The aim of this study was to determine whether ATP-binding cassette transporter A1 (ABCA1) was up-regulated by growth differentiation factor-15 (GDF-15) via the phosphoinositide 3-kinase (PI3K)/protein kinase Cζ (PKCζ)/specificity protein 1 (SP1) pathway in THP-1 macrophages. METHODS AND RESULTS:We investiga...

journal_title：Biochemical and biophysical research communications

authors： Wu JF,Wang Y,Zhang M,Tang YY,Wang B,He PP,Lv YC,Ouyang XP,Yao F,Tan YL,Tang SL,Tang DP,Cayabyab FS,Zheng XL,Zhang DW,Zeng GF,Tang CK

更新日期：2014-02-14 00:00:00

abstract：:Rab5 is a GTP-binding protein that is crucial for endocytic machinery functions. We previously identified L-plastin as a binding protein for Rab5, using an affinity column with constitutively active Rab5. L- and T-plastin are isoforms of a plastin protein family belonging to actin-bundling proteins that are implicated...

journal_title：Biochemical and biophysical research communications

authors： Hagiwara M,Shinomiya H,Kashihara M,Kobayashi K,Tadokoro T,Yamamoto Y

更新日期：2011-04-15 00:00:00

abstract：:This study aims to investigate the clinical significance and biological function of RASSF6 in human breast cancers. RASSF6 protein was found to be downregulated in 42 of 95 human breast cancer tissues by immunohistochemistry, which was associated with advanced TNM stage and nodal metastasis. The rate of RASSF6 downreg...

journal_title：Biochemical and biophysical research communications

authors： He Z,Zhao TT,Jin F,Li JG,Xu YY,Dong HT,Liu Q,Xing P,Zhu GL,Xu H,Miao ZF

更新日期：2018-09-18 00:00:00

abstract：:Treatment of colorectal cancer (CRC) remains a challenge because of the lack of effective early treatment strategies and high incidence of relapse. 5-Fluorouracil (5-FU) is a typical CRC treatment. Bromosporine is an innovative bromodomain and extraterminal domain (BET) inhibitor. We investigated if CRC could be targe...

journal_title：Biochemical and biophysical research communications

authors： Cheng X,Huang Z,Long D,Jin W

更新日期：2020-01-22 00:00:00