Silkworm hemolymph as a potent inhibitor of apoptosis in Sf9 cells.

Abstract:

:We have previously shown that silkworm hemolymph exhibits anti-apoptotic activity against baculovirus-induced Sf9 cell apoptosis. In this study, using various chemical inducers, such as actinomycin D, camptothecin, and staurosporine, we found that silkworm hemolymph inhibits insect cell apoptosis induced not only by baculovirus but also by chemical inducers. This indicates that silkworm hemolymph contains anti-apoptotic components that work directly in insect cell apoptosis without any booster expression of baculoviral genes. With the analysis of Sf-caspase-1 activity, it was found that the inhibitory effect of silkworm hemolymph works in a further upstream step than the Sf-caspase-1 activation step.

authors

Rhee WJ,Kim EJ,Park TH

doi

10.1016/s0006-291x(02)00746-5

subject

Has Abstract

pub_date

2002-07-26 00:00:00

pages

779-83

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006291X02007465

journal_volume

295

pub_type

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