Paxillin localisation in osteocytes--is it determined by the direction of loading?

Abstract:

:External mechanical loading of cells aligns cytoskeletal stress fibres in the direction of principle strains and localises paxillin to the mechanosensing region. If the osteocyte cell body can indeed directly sense matrix strains, then cytoskeletal alignment and distribution of paxillin in osteocytes in situ will bear alignment to the different mechanical loading patterns in fibulae and calvariae. We used confocal microscopy to visualise the immunofluorescence-labelled actin cytoskeleton in viable osteocytes and paxillin distribution in fixated osteocytes in situ. In fibular osteocyte cell bodies, actin cytoskeleton and nuclei were elongated and aligned parallel to the principal (longitudinal) mechanical loading direction. Paxillin was localised to the 'poles' of elongated osteocyte cell bodies. In calvarial osteocyte cell bodies, actin cytoskeleton and nuclei were relatively more round. Paxillin was distributed evenly in the osteocyte cell bodies. Thus in osteocyte cell bodies in situ, the external mechanical loading pattern likely determines the orientation of the actin cytoskeleton, and focal adhesions mediate direct mechanosensation of matrix strains.

authors

Vatsa A,Semeins CM,Smit TH,Klein-Nulend J

doi

10.1016/j.bbrc.2007.12.174

subject

Has Abstract

pub_date

2008-12-26 00:00:00

pages

1019-24

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)02814-8

journal_volume

377

pub_type

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