Syndecan-2 enhances E-cadherin shedding and fibroblast-like morphological changes by inducing MMP-7 expression in colon cancer cells.

Abstract:

:E-cadherin plays a mechanical role in mediating cell-cell interactions and maintaining epithelial tissue integrity, and the loss of E-cadherin function has been implicated in cancer progression and metastasis. Syndecan-2, a cell-surface heparan sulfate proteoglycan, is upregulated during the development of colon cancer. Here, we assessed the functional relationship between E-cadherin and syndecan-2. We found that stable overexpression of syndecan-2 in a human colorectal adenocarcinoma cell line (HT29) enhanced the proteolytic shedding of E-cadherin to conditioned-media. Either knockdown of matrix metalloproteinase 7 (MMP-7) or inhibition of MMP-7 activity using GM6001 significantly reduced the extracellular shedding of E-cadherin, suggesting that syndecan-2 mediates E-cadherin shedding via MMP-7. Consistent with this notion, enhancement of MMP-7 expression by interleukin-1α treatment increased the shedding of E-cadherin. Conversely, the specific reduction of either syndecan-2 or MMP-7 reduced the shedding of E-cadherin. HT29 cells overexpressing syndecan-2 showed significantly lower cell-surface expression of E-cadherin, decreased cell-cell contact, a more fibroblastic cell morphology, and increased expression levels of ZEB-1. Taken together, these data suggest that syndecan-2 induces extracellular shedding of E-cadherin and supports the acquisition of a fibroblast-like morphology by regulating MMP-7 expression in a colon cancer cell line.

authors

Jang B,Jung H,Chung H,Moon BI,Oh ES

doi

10.1016/j.bbrc.2016.06.019

subject

Has Abstract

pub_date

2016-08-12 00:00:00

pages

47-53

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(16)30937-8

journal_volume

477

pub_type

杂志文章
  • Identification of a novel determinant of glutathione affinity in dichloromethane dehalogenases/glutathione S-transferases.

    abstract::Bacterial dichloromethane dehalogenases catalyze the glutathione-dependent hydrolysis of dichloromethane to formaldehyde and are members of the enzyme superfamily of glutathione S-transferases involved in the detoxification of electrophilic compounds. Numerous protein engineering studies have addressed questions perta...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.7309

    authors: Vuilleumier S,Sorribas H,Leisinger T

    更新日期:1997-09-18 00:00:00

  • Dexamethasone affects phosphatidylinositol synthesis and degradation in cultured human embryonic cells.

    abstract::Dexamethasone (DEX), a glucocorticoid which induces cleft palate, causes marked alterations in the synthesis and degradation of phosphatidylinositol (PI) but not phosphatidylcholine in an established fibroblastic cell line derived from a human embryonic palate. Incorporation of radiolabeled inositol into phosphatidyli...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)91280-9

    authors: Grove RI,Willis WD,Pratt RM

    更新日期:1983-01-14 00:00:00

  • Human cartilage from late stage familial osteoarthritis transcribes type II collagen mRNA encoding a cysteine in position 519.

    abstract::A single base change resulting in the substitution of Cys for Arg at position 519 of the type II collagen triple helix is a predisposing factor in the pathogenesis of a precocious-onset form of familial osteoarthritis associated with a mild chondrodysplasia. Cartilage obtained at the time of total knee replacement in ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.1539

    authors: Holderbaum D,Malemud CJ,Moskowitz RW,Haqqi TM

    更新日期:1993-05-14 00:00:00

  • Transgenic overexpression of USP15 in the heart induces cardiac remodeling in mice.

    abstract::We found a novel protein-protein interaction between ubiquitin-specific protease 15 (USP15) and skeletal muscle LIM protein 1 (SLIM1): USP15 and SLIM1 directly bound under cell-free conditions and co-immunoprecipitated from the lysates of the cells, where they were co-expressed; and USP15 deubiquitinated SLIM1, result...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2011.01.012

    authors: Isumi Y,Hirata T,Saitoh H,Miyakawa T,Murakami K,Kudoh G,Doi H,Ishibashi K,Nakajima H

    更新日期:2011-02-11 00:00:00

  • Factor C-LHIH which inhibits the luteinizing hormone from basal release and from synthetic LHRH and studies on purification of FSHRH.

    abstract::Data on a new entity which inhibits the release of luteinizing hormone (LH), designated as factor C-LHIH, are presented. In vitro assays were carried out with pituitary glands from female rats. Factor C-LHIH and follicle stimulating hormone releasing hormone (FSHRH) initially fractionated together. FSHRH obscured C...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(75)80011-8

    authors: Johansson KN,Greibrokk T,Currie BL,Hansen J,Folkers K

    更新日期:1975-03-03 00:00:00

  • Preliminary assessment of the C13-side chain 2'-hydroxylase involved in taxol biosynthesis.

    abstract::The biosynthesis of the anticancer drug Taxol in yew (Taxus) species is thought to involve the preliminary formation of the advanced taxane diterpenoid intermediate baccatin III upon which the functionally important N-benzoyl phenylisoserinoyl side chain is subsequently assembled at the C13-O-position. In vivo feeding...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.08.119

    authors: Long RM,Croteau R

    更新日期:2005-12-09 00:00:00

  • The unit event of sliding of the chemo-mechanical enzyme composed of myosin and actin with regulatory proteins.

    abstract::Various myosin-actin systems do not always show the same sliding behaviors. To make the situation clear, discussions are concentrated on the unit event of sliding of the chemo-mechanical enzyme composed of a single myosin head and a single actin filament with regulatory proteins. The popular idea of the one-to-one cor...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审

    doi:10.1016/j.bbrc.2007.11.186

    authors: Oosawa F

    更新日期:2008-04-25 00:00:00

  • Enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for molecular detection of M. tuberculosis.

    abstract::An enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification (NASBA-ELISA) was developed for molecular detection of Mycobacterium tuberculosis. The primers targeting 16S rRNA were used for the amplification of bacterial RNA by the isothermal digoxigenin (DIG)-labeling NASBA process, resulting in t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.07.039

    authors: Gill P,Ramezani R,Amiri MV,Ghaemi A,Hashempour T,Eshraghi N,Ghalami M,Tehrani HA

    更新日期:2006-09-08 00:00:00

  • Unraveling the molecular mechanism of BNC105, a phase II clinical trial vascular disrupting agent, provides insights into drug design.

    abstract::Microtubules are made up of tubulin protein and play a very important part in numerous cellular events of eukaryotic cells, which is why they are seen as attractive targets for tumor chemotherapy. BNC105, a known vascular targeting agent, has entered in phase II clinical trials. It has previously been confirmed that B...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.12.083

    authors: Wang T,Wu C,Wang C,Zhang G,Arnst KE,Yao Y,Zhang Z,Wang Y,Pu D,Li W

    更新日期:2020-02-18 00:00:00

  • Demonstration of histamine H2 receptors on human melanoma cells.

    abstract::Histamine induced a concentration-dependent increase in intracellular cyclic-AMP of the two human melanoma cell lines SK23 and DX3.LT5.1; maximal stimulation was obtained with 17.8 microM histamine which consistently produced greater than 50-fold increases in the cyclic AMP content of both cell lines. The dose-respons...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(88)90624-9

    authors: Whitehead RJ,Taylor DJ,Evanson JM,Hart IR,Woolley DE

    更新日期:1988-02-29 00:00:00

  • Tyrosine residues 239 and 240 of Shc are phosphatidylinositol 4,5-bisphosphate-dependent phosphorylation sites by c-Src.

    abstract::In the previous study (Sato K.-I. et al. (1997) FEBS Lett. 410, 136-140), we showed that the phosphorylation of Shc protein by c-Src is dependent on the binding of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to the PTB domain of Shc. In this study, we demonstrate that, in contrast to c-Src, v-Src and epiderm...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.7667

    authors: Sato K,Gotoh N,Otsuki T,Kakumoto M,Aoto M,Tokmakov AA,Shibuya M,Fukami Y

    更新日期:1997-11-17 00:00:00

  • Complementary DNA cloning and nucleotide sequence of rabbit serum amyloid A protein.

    abstract::A complementary DNA clone encoding serum amyloid A protein has been isolated from an acute rabbit liver cDNA library. Complete nucleotide sequence analysis reveals that the cloned gene contains a 24 bases 5' untranslated region, 369 bases coding region and a 106 bases 3' untranslated region. Primer extension analysis ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)91780-g

    authors: Ray BK,Ray A

    更新日期:1991-07-15 00:00:00

  • Novel protein-protein interaction between spermidine synthase and S-adenosylmethionine decarboxylase from Leishmania donovani.

    abstract::Polyamine biosynthesis pathway has long been considered an essential drug target for trypanosomatids including Leishmania. S-adenosylmethionine decarboxylase (AdoMetDc) and spermidine synthase (SpdSyn) are enzymes of this pathway that catalyze successive steps, with the product of the former, decarboxylated S-adenosyl...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.12.008

    authors: Mishra AK,Agnihotri P,Srivastava VK,Pratap JV

    更新日期:2015-01-09 00:00:00

  • Lipid peroxidation as a possible cause of TCDD toxicity.

    abstract::The target tissues of TCDD, the dysfunctions that result in death in experimental animals, and the ultimate biochemical lesion(s) caused by TCDD are not known despite numerous studies. We have shown by the thiobarbituric acid and conjugated diene methods that TCDD induces hepatic lipid peroxidation in rats. The lipid ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)91377-3

    authors: Stohs SJ,Hassan MQ,Murray WJ

    更新日期:1983-03-29 00:00:00

  • Suppression of Akt1 phosphorylation by adenoviral transfer of the PTEN gene inhibits hypoxia-induced proliferation of rat pulmonary arterial smooth muscle cells.

    abstract::Recent findings identify the role of proliferation of pulmonary artery smooth muscle cells (PASMCs) in pulmonary vascular remodeling. Phosphoinositide 3 kinase (PI3K) and serine/threonine kinase (Akt) proteins are expressed in vascular smooth muscle cells. In addition, phosphatase and tensin homolog deleted on chromos...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.05.140

    authors: Luo C,Yi B,Bai L,Xia Y,Wang G,Qian G,Feng H

    更新日期:2010-07-02 00:00:00

  • Expression of the ED B fibronectin isoform in adult human articular cartilage.

    abstract::The most recently described region of alternate splicing of fibronectin mRNA results in expression of an isoform which includes the type III repeat termed ED B (EIII B). To date this isoform has been detected in transformed cells in culture, in the synovial membrane and ovary but in no other adult tissue, and in embry...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(89)80034-8

    authors: Burton-Wurster N,Lust G,Wert R

    更新日期:1989-12-15 00:00:00

  • AMPK activation by pterostilbene contributes to suppression of hepatic gluconeogenic gene expression and glucose production in H4IIE cells.

    abstract::Pterostilbene, a bioactive component of blueberries and grapes, shows structural similarity to resveratrol, and exhibits antioxidant, anti-inflammatory, anti-cancer, hypoglycemic, and cholesterol lowering effects. Recent evidence indicates that pterostilbene is an agonist of the nuclear receptor, peroxisome proliferat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.03.035

    authors: Ren G,Rimando AM,Mathews ST

    更新日期:2018-04-06 00:00:00

  • Polyprenyl lipid synthesis in mammalian cells expressing human cis-prenyl transferase.

    abstract::The level of cis-prenyl transferase activity has been implicated in controlling the level of biosynthesis of dolichol and dolichol intermediates. In this study, we isolated a cDNA encoding a human CPT (GenBank Accession No. ), which had substantial homology to other CPT isolated from human brain, bacteria, Arabidopsis...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.03.181

    authors: Jones J,Viswanathan K,Krag SS,Betenbaugh MJ

    更新日期:2005-06-03 00:00:00

  • VEGF-A and VEGF-F evoke distinct changes in vascular ultrastructure.

    abstract::VEGF-induced vascular barrier disruptions, formation of fenestrae and vesiculo-vacuolar organelles (VVOs), are involved in enhancing vascular permeability in metastatic and edematous diseases. Here, we analyzed vascular permeability enhanced by VEGFs with different receptor selectivity using biological and ultrastruct...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.12.129

    authors: Matsunaga Y,Yamazaki Y,Suzuki H,Morita T

    更新日期:2009-02-20 00:00:00

  • Glycyl-tRNA synthetase from Nanoarchaeum equitans: The first crystal structure of archaeal GlyRS and analysis of its tRNA glycylation.

    abstract::This study reports the X-ray crystallographic structure of the glycyl-tRNA synthetase (GlyRS) of Nanoarchaeum equitans - a hyperthermophilic archaeal species. This is the first archaeal GlyRS crystal structure elucidated. The GlyRS comprises an N-terminal catalytic domain and a C-terminal anticodon-binding domain with...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.01.142

    authors: Fujisawa A,Toki R,Miyake H,Shoji T,Doi H,Hayashi H,Hanabusa R,Mutsuro-Aoki H,Umehara T,Ando T,Noguchi H,Voet A,Park SY,Tamura K

    更新日期:2019-04-02 00:00:00

  • microRNA-146a inhibits proliferation, migration and invasion of human cervical and colorectal cancer cells.

    abstract::microRNAs (miRNAs) play significant roles in diverse biological processes and their deregulation is implicated in carcinogenesis. miR-146a executes tumour suppressive or oncogenic functions depending on the cancer type, but its effect on human cervical (CaCx) and colorectal (CRC) cancers have not been examined thus fa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.10.054

    authors: Sathyanarayanan A,Chandrasekaran KS,Karunagaran D

    更新日期:2016-11-25 00:00:00

  • A high-sensitive HMab-2 specifically detects IDH1-R132H, the most common IDH mutation in gliomas.

    abstract::Isocitrate dehydrogenase 1 (IDH1) mutations have been detected in gliomas and other tumors. Although IDH1 catalyzes the oxidative carboxylation of isocitrate to α-ketoglutarate (α-KG) in cytosol, mutated IDH1 proteins possess the ability to change α-KG into the oncometabolite D-2-hydroxyglutarate (D-2HG). Several mono...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.09.070

    authors: Fujii Y,Ogasawara S,Oki H,Liu X,Kaneko MK,Takano S,Kato Y

    更新日期:2015-10-30 00:00:00

  • Characterization of calcineurin-dependent response element binding protein and its involvement in copper-metallothionein gene expression in Neurospora.

    abstract::In continuation of our recent observations indicating the presence of a lone calcineurin-dependent response element (CDRE) in the -3730bp upstream region of copper-induced metallothionein (CuMT) gene of Neurospora [K.S. Kumar, S. Dayananda, C. Subramanyam, Copper alone, but not oxidative stress, induces copper-metallo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.05.010

    authors: Kumar KS,Ravi Kumar B,Siddavattam D,Subramanyam C

    更新日期:2006-07-07 00:00:00

  • Characterisation of two novel CYP4 genes from the marine polychaete Nereis virens and their involvement in pyrene hydroxylase activity.

    abstract::Cytochrome P450 enzymes (CYP enzymes) catalyse the initial step in biotransformation of xenobiotics like polycyclic aromatic hydrocarbons (PAHs). The marine polychaete Nereis virens has a high capacity for biotransformation of PAHs. In the present study, the complete cDNA sequences of two novel CYP genes isolated from...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.08.189

    authors: Jørgensen A,Rasmussen LJ,Andersen O

    更新日期:2005-10-28 00:00:00

  • Nanosecond electric pulses trigger actin responses in plant cells.

    abstract::We have analyzed the cellular effects of nanosecond pulsed electrical fields on plant cells using fluorescently tagged marker lines in the tobacco cell line BY-2 and confocal laser scanning microscopy. We observe a disintegration of the cytoskeleton in the cell cortex, followed by contraction of actin filaments toward...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.07.072

    authors: Berghöfer T,Eing C,Flickinger B,Hohenberger P,Wegner LH,Frey W,Nick P

    更新日期:2009-09-25 00:00:00

  • One in vitro model for visceral adipose-derived fibroblasts in chronic inflammation.

    abstract::One pathogenesis of the obesity-associated complications is that consistent with increased body fat mass, the elevation of adipose tissue-derived cytokines inflicts a low-grade chronic inflammation, which ultimately leads to metabolic disorders. Adipocytes and macrophages in visceral adipose (VA) have been confirmed t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.05.182

    authors: Yue GP,Du LR,Xia T,He XH,Qiu H,Xu LH,Chen XD,Feng SQ,Yang ZQ

    更新日期:2005-08-05 00:00:00

  • Cell cycle dependent telomere regulation by telomerase in human bone marrow mesenchymal stem cells.

    abstract::Human bone marrow mesenchymal stem cells (hMSCs) are a promising source for clinical stem cell transplantation. However, telomere regulation mechanisms, as one of the possible major mechanisms by which hMSCs sustain their stem cell characteristics, remain unknown. We isolated hMSCs by plastic adhesion and characterize...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.03.011

    authors: Zhao YM,Li JY,Lan JP,Lai XY,Luo Y,Sun J,Yu J,Zhu YY,Zeng FF,Zhou Q,Huang H

    更新日期:2008-05-16 00:00:00

  • Lifetime evidence for a weak lowest electronic transition in adenosine.

    abstract::The fluorescence decay of adenosine in 1:1 glycol/water glass has been determined at 77K using narrow pulse (700 ps) laser excitation at 290 nm and fluorescence detection with a scanned narrow-gate (100 ps) fast sampler together with digital averaging. Data analysis by re-iterative non-linear least squares convolution...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)92378-4

    authors: Hart LP,Daniels M

    更新日期:1989-07-31 00:00:00

  • Both stimulatory and inhibitory GDP/GTP exchange proteins, smg GDS and rho GDI, are active on multiple small GTP-binding proteins.

    abstract::Six peaks of small GTP-binding proteins (G proteins) were separated by column chromatographies from the cytosol fraction of the differentiated HL-60 cells: two peaks of rho p21, one peak of smg/rap1 p21, two peaks of rac1 p21, and one peak of an unidentified small G protein with a Mr of about 20,000 (20 KG). smg GDS, ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(92)91820-g

    authors: Hiraoka K,Kaibuchi K,Ando S,Musha T,Takaishi K,Mizuno T,Asada M,Ménard L,Tomhave E,Didsbury J

    更新日期:1992-01-31 00:00:00

  • Down-regulation of TRAF4 targeting RSK4 inhibits proliferation, invasion and metastasis in breast cancer xenografts.

    abstract::Ribosomal S6 protein kinase 4 (RSK4) was known as a novel tumor suppressor gene, and the tumor necrosis factor receptor-associated factor 4 (TRAF4) was linked to carcinogenesis. The purpose of this study is to further investigate the effect of the TRAF4 gene on cell proliferation, invasion and metastasis in vivo and e...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.04.164

    authors: Zhu L,Zhang S,Huan X,Mei Y,Yang H

    更新日期:2018-06-07 00:00:00