当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > A sugarcane cystatin: recombinant expression, purification, and antifungal activity.

A sugarcane cystatin: recombinant expression, purification, and antifungal activity.

Abstract：

:Plants possess several defense mechanisms against pathogenic attack. One of these defenses is the use of protease inhibitor proteins, which interfere in the development and growth of pathogens. Sugarcane productivity can be impacted by the plant's susceptibility to fungal diseases that result in production losses. A relevant line of investigation, therefore, is into the plant's natural defense mechanisms for the control of phytopathogens using cystatins-proteins that specifically inhibit cysteine proteases. In this paper, we discuss the expression, in Escherichia coli, of a sugarcane cystatin, its purification, antifungal activity, and circular dichroism to monitor correct folding. These studies revealed a secondary structure similar to that of the oryzacystatin I of rice. Moreover, the purified protein proved capable of inhibiting the growth of the filamentous fungus Trichoderma reesei, suggesting that it can also be employed to inhibit the growth of pathogenic sugarcane fungi.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Soares-Costa A,Beltramini LM,Thiemann OH,Henrique-Silva F

doi

10.1016/s0006-291x(02)02046-6

subject

Has Abstract

pub_date

2002-09-06 00:00:00

pages

1194-9

issue

5

eissn

0006-291X

issn

1090-2104

pii

S0006291X02020466

journal_volume

296

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Stabilization of C-terminal binding protein 2 by cellular inhibitor of apoptosis protein 1 via BIR domains without E3 ligase activity.

    abstract：:C-terminal binding protein 2 (CtBP2) is a transcriptional co-repressor that regulates many genes involved in normal cellular events. Because CtBP2 overexpression has been implicated in various human cancers, its protein levels must be precisely regulated. Previously, we reported that CtBP1 and CtBP1-mediated transcrip...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.05.098

    authors： Seo TW,Lee YT,Lee JS,Yoo SJ

    更新日期：2020-09-17 00:00:00

  • Ca2+-dependent interaction between calmodulin and CoDN3, an effector of Colletotrichum orbiculare.

    abstract：:Nuclear magnetic resonance (NMR) data directly indicated a Ca2+-dependent interaction between calmodulin (CaM) and CoDN3, a small effector of the plant pathogenic fungus Colletotrichum orbiculare, which is the causal agent of cucumber anthracnose. The overall conformation of CoDN3 is intrinsically disordered, and the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.05.007

    authors： Isozumi N,Inoue Y,Imamura T,Mori M,Takano Y,Ohki S

    更新日期：2019-06-30 00:00:00

  • Beneficial effects of IKKε-deficiency on body weight and insulin sensitivity are lost in high fat diet-induced obesity in mice.

    abstract：:Activation of the classical IκB kinases (IKKα and IKKβ) was previously shown to contribute to obesity-induced inflammation and insulin resistance. Using knockout mice, we investigated whether the related isoform IKKε plays a similar metabolic role. IKKε(-/-) mice had reduced body weight, leptin levels, as well as high...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.02.137

    authors： Scheja L,Heese B,Seedorf K

    更新日期：2011-04-08 00:00:00

  • Evidence for lateral mobility of voltage sensors in prokaryotic voltage-gated sodium channels.

    abstract：:Voltage-sensor domains (VSDs) in voltage-gated ion channels are thought to regulate the probability that a channel adopts an open conformation by moving vertically in the lipid bilayer. Here we characterized the movement of the VSDs of the prokaryotic voltage-gated sodium channel, NaChBac. Substitution of residue T110...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.07.070

    authors： Nagura H,Irie K,Imai T,Shimomura T,Hige T,Fujiyoshi Y

    更新日期：2010-08-27 00:00:00

  • A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans.

    abstract：:Cycloprodigiosin hydrochloride (cPrG.HCl), a member of the prodigiosin family, is a red pigment obtained from the marine bacterium Pseudoalteromonas denitrificans. cPrG.HCl markedly suppressed 3H-thymidine incorporation by concanavalin A stimulated murine splenocytes but had little effect on lipopolysaccharide depende...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7186

    authors： Kawauchi K,Shibutani K,Yagisawa H,Kamata H,Nakatsuji S,Anzai H,Yokoyama Y,Ikegami Y,Moriyama Y,Hirata H

    更新日期：1997-08-28 00:00:00

  • Coordinate regulation/localization of the carbohydrate responsive binding protein (ChREBP) by two nuclear export signal sites: discovery of a new leucine-rich nuclear export signal site.

    abstract：:Carbohydrate response element binding protein (ChREBP) is responsible for conversion of dietary carbohydrate to storage fat in liver by coordinating expression of the enzymes that channel glycolytic pyruvate into lipogenesis. The activation of ChREBP in response to high glucose is nuclear localization and transcriptio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.11.115

    authors： Fukasawa M,Ge Q,Wynn RM,Ishii S,Uyeda K

    更新日期：2010-01-08 00:00:00

  • Lectin affinity electrophoretic demonstration of tissue specificity and malignant alteration of human alpha-fetoprotein isoforms produced in transgenic mice.

    abstract：:Transgenic mouse which produces human alpha-fetoprotein (AFP) ubiquitously was used to study carbohydrate structures of AFP produced by various tissues as well as that in the serum. A series of tissues from the transgenic mouse were cultured in vitro and the AFP produced was analyzed by affinity electrophoreses with 4...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0969

    authors： Koyama Y,Sakai M,Kitagawa T,Taketa K,Nishi S

    更新日期：1996-06-25 00:00:00

  • Molecular imprinting: synthesis of polymer particles with antibody-like binding characteristics for glucose oxidase.

    abstract：:A phosphate buffer containing a mixture of glucose oxidase, acrylic acid derivatives, N,N'-1,2 dihydroxy-ethylene-bis(acrylamide), N,N'-(methylene)-bisacrylamide and surface-modified silica was radically polymerized with (NH4)2S2O8. The polymer formed a thin layer around the silica beads. After sieving of these polyme...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1522

    authors： Burow M,Minoura N

    更新日期：1996-10-14 00:00:00

  • Induction and regulation of CD8+ cytolytic T cells in human tuberculosis and HIV infection.

    abstract：:Tuberculosis and HIV continue to be the world-leading killers among infectious diseases, primarily affecting poor people in many developing countries. Despite differences in the immunopathogenesis of human infection with tuberculosis and HIV, experimental evidence from clinical studies and relevant animal models can b...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2010.02.141

    authors： Brighenti S,Andersson J

    更新日期：2010-05-21 00:00:00

  • Multiple copies of beta-lactoglobulin promoter do not function as LCR.

    abstract：:Increasing the number of transcription factor binding sites within a construct can enhance expression. In an attempt to create a synthetic locus control region for mammary expression, we have generated beta-lactoglobulin-reporter constructs with multiple copies of the cluster of transcription sites normally located wi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2766

    authors： James RM,Neil C,Webster J,Roos S,Clark AJ,Whitelaw CB

    更新日期：2000-05-27 00:00:00

  • In vitro transcription termination activity of the Drosophila mitochondrial DNA-binding protein DmTTF.

    abstract：:DmTTF is a Drosophila melanogaster mitochondrial DNA-binding protein which binds specifically to two homologous non-coding sequences located at the 3' ends of blocks of genes encoded on opposite strands. In order to test whether this protein acts as transcription termination factor, we assayed the capacity of DmTTF to...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.173

    authors： Roberti M,Fernandez-Silva P,Polosa PL,Fernandez-Vizarra E,Bruni F,Deceglie S,Montoya J,Gadaleta MN,Cantatore P

    更新日期：2005-05-27 00:00:00

  • Mechanism involved in the antiplatelet activity of naloxone in human platelets.

    abstract：:In this study, naloxone was tested for its antiplatelet activity in human platelet suspensions. In platelet suspensions (4.5 x 10(8)/ml), naloxone (0.1-0.5 mM) significantly inhibited platelet aggregation and ATP-release stimulated by various agonists (i.e., thrombin, collagen, U46619, and ADP). Furthermore, naloxone ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.6037

    authors： Sheu JR,Hung WC,Lee LW,Chang PT,Kan YC,Yen MH

    更新日期：1997-02-03 00:00:00

  • The use of the novel substrate-heme complex approach in the derivation of a representation of the active site of the enzyme cholesterol side chain cleavage.

    abstract：:The previously reported substrate-heme complex approach is used to study the binding of type II inhibitors of the enzyme cholesterol side chain cleavage (CSCC), a cytochrome P-450 dependent enzyme involved in the oxidative cleaveage of the C(20)-C(22) bond of cholesterol. Using the derived model, we have rationalised ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3209

    authors： Ahmed S

    更新日期：2000-08-11 00:00:00

  • MicroRNA-320a suppresses human colon cancer cell proliferation by directly targeting β-catenin.

    abstract：:Recent profile studies of microRNA (miRNA) expression have documented a deregulation of miRNA (miR-320a) in human colorectal carcinoma. However, its expression pattern and underlying mechanisms in the development and progression of colorectal carcinoma has not been elucidated clearly. Here, we performed real-time PCR ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.03.075

    authors： Sun JY,Huang Y,Li JP,Zhang X,Wang L,Meng YL,Yan B,Bian YQ,Zhao J,Wang WZ,Yang AG,Zhang R

    更新日期：2012-04-20 00:00:00

  • Transgene integration into the human AAVS1 locus enhances myosin II-dependent contractile force by reducing expression of myosin binding subunit 85.

    abstract：:The adeno-associated virus site 1 (AAVS1) locus in the human genome is a strong candidate for gene therapy by insertion of an exogenous gene into the locus. The AAVS1 locus includes the coding region for myosin binding subunit 85 (MBS85). Although the function of MBS85 is not well understood, myosin II-dependent contr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.08.018

    authors： Mizutani T,Li R,Haga H,Kawabata K

    更新日期：2015-09-18 00:00:00

  • Carbon monoxide exposure in rat heart: glutathione depletion is prevented by antioxidants.

    abstract：:Rat hearts were perfused for 15min with buffer equilibrated with 0.01% or 0.05% CO. The buffer was equilibrated with 21% O(2) throughout. The ventricular glutathione content decreased by 76% and 84%, 90min post-exposure to 0.01% and 0.05% CO, respectively, compared with 0% CO controls (0.45+/-0.01 micromol/g wet tissu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00190-6

    authors： Patel AP,John Moody A,Handy RD,Robert Sneyd J

    更新日期：2003-03-07 00:00:00

  • Silica-induced generation of reactive oxygen species in Rat2 fibroblast: role in activation of mitogen-activated protein kinase.

    abstract：:Exposure to silica has been associated with progressive pulmonary inflammation and fibrosis. While the fibroblasts play an important role in the pathogenesis of silicosis, the direct interaction between silica and fibroblasts is poorly understood. We observed that silica particles stimulated intracellular ROS generati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1274

    authors： Cho YJ,Seo MS,Kim JK,Lim Y,Chae G,Ha KS,Lee KH

    更新日期：1999-09-07 00:00:00

  • Vascular actions of C-type natriuretic peptide in isolated porcine coronary arteries and coronary vascular smooth muscle cells.

    abstract：:C-type natriuretic (CNP) caused concentration-dependent relaxations in porcine coronary arteries with a maximal relaxation (10(-6)M) of 46%. Relaxations to CNP in isolated coronary arteries were significantly attenuated with potassium channel antagonists charybdotoxin (10(-7)M) and glibenclamide (10(-7)M). Membrane po...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2731

    authors： Wei CM,Hu S,Miller VM,Burnett JC Jr

    更新日期：1994-11-30 00:00:00

  • Rat copper/zinc superoxide dismutase gene: isolation, characterization, and species comparison.

    abstract：:A 13 kb rat Cu/ZnSOD genomic clone has been purified from a rat liver genomic library and completely characterized by restriction mapping, detailed sequencing and Southern blot analysis. This gene spans approximately 6 kb and contains five exons and four introns. Comparison of rat, mouse, and human Cu/ZnSOD genes reve...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)90836-a

    authors： Hsu JL,Visner GA,Burr IA,Nick HS

    更新日期：1992-07-31 00:00:00

  • Role of S6K1 in regulation of SREBP1c expression in the liver.

    abstract：:The transcription factor sterol regulatory element-binding protein 1c (SREBP1c) plays an important role in the control of fatty acid metabolism in the liver. Evidence suggests that mammalian target of rapamycin (mTOR) complex 1 (mTORC1) contributes to the regulation of SREBP1c expression, but signaling downstream of m...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.07.038

    authors： Li S,Ogawa W,Emi A,Hayashi K,Senga Y,Nomura K,Hara K,Yu D,Kasuga M

    更新日期：2011-08-26 00:00:00

  • Antigen-specific CD8+ T cells induced by the ubiquitin fusion degradation pathway.

    abstract：:We have developed a DNA vaccine encoding a fusion protein of ubiquitin (Ub) and target proteins at the N-terminus for effective induction of antigen-specific CD8(+) T cells. A series of expression plasmids encoding a model antigen, ovalbumin (OVA), fused with mutated Ub, was constructed. Western blotting analyses usin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.11.034

    authors： Imai T,Duan X,Hisaeda H,Himeno K

    更新日期：2008-01-25 00:00:00

  • Differential regulation of rat AT1a and AT1b receptor mRNA.

    abstract：:Rat type 1 angiotensin II receptor has two subtypes, namely type 1a and type 1b. The regulation of the expressions of these two subtype receptor mRNAs was studied by using a competitive polymerase chain reaction method. The expression of the type 1a mRNA in the liver was negatively and that of the type 1b mRNA in the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)92384-a

    authors： Iwai N,Inagami T,Ohmichi N,Nakamura Y,Saeki Y,Kinoshita M

    更新日期：1992-10-15 00:00:00

  • DISC1-kendrin interaction is involved in centrosomal microtubule network formation.

    abstract：:Disrupted-In-Schizophrenia 1 (DISC1) was identified as a novel gene disrupted by a (1;11)(q42.1;q14.3) translocation segregating with schizophrenia, bipolar disorder and other major mental illnesses in a Scottish family. We previously identified 446-533 amino acids of DISC1 as the kendrin-binding region by means of a ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.10.100

    authors： Shimizu S,Matsuzaki S,Hattori T,Kumamoto N,Miyoshi K,Katayama T,Tohyama M

    更新日期：2008-12-26 00:00:00

  • CTRP3 plays an important role in the development of collagen-induced arthritis in mice.

    abstract：:Rheumatoid arthritis (RA) is an autoimmune inflammatory disease exhibited most commonly in joints. We found that the expression of C1qtnf3, which encodes C1q/TNF-related protein 3 (CTRP3), was highly increased in two mouse RA models with different etiology. To elucidate the pathogenic roles of CTRP3 in the development...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.11.040

    authors： Murayama MA,Kakuta S,Maruhashi T,Shimizu K,Seno A,Kubo S,Sato N,Saijo S,Hattori M,Iwakura Y

    更新日期：2014-01-03 00:00:00

  • Intestinal Pdx1 mediates nutrient metabolism gene networks and maternal expression is essential for perinatal growth in mice.

    abstract：:The homeodomain transcription factor Pdx1 is essential for pancreas formation and functions in pancreatic islets cells to regulate genes involved in maintenance of glucose homeostasis. In order to investigate a role for Pdx1 in intestinal cells, we analyzed the functions and networks associated with genes differential...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.06.153

    authors： Chen C,Leavitt T,Sibley E

    更新日期：2012-08-03 00:00:00

  • Prosomatostatin processing in anglerfish brain, gut and pancreas.

    abstract：:The distribution of somatostatin immunoreactive forms in three tissues of the anglerfish (Lophius piscatorius L.) was analyzed by a combination of gel permeation, High Pressure Liquid Chromatography and amino acid analysis. The data indicate that prosomatostatins I and II are expressed in both neural and gastro-intest...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90600-6

    authors： Morel A,Kuks PF,Bourdais J,Cohen P

    更新日期：1988-02-29 00:00:00

  • Protein kinase A mediates cAMP-induced tyrosine phosphorylation of the epidermal growth factor receptor.

    abstract：:An increase in the intracellular cAMP concentration induces tyrosine phosphorylation of the epidermal growth factor receptor (EGFR) followed by activation of extracellular signal-regulated kinases 1/2 (ERK1/2). In this report we demonstrate that these effects of cAMP are mediated via activation of protein kinase A (PK...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00055-x

    authors： Piiper A,Lutz MP,Cramer H,Elez R,Kronenberger B,Dikic I,Müller-Esterl W,Zeuzem S

    更新日期：2003-02-21 00:00:00

  • Transplantation of dermal fibroblasts expressing MyoD1 in mouse muscles.

    abstract：:Transplantation of normal myoblasts into dystrophic muscles is a potential treatment for Duchenne muscular dystrophy (DMD). However, the success of such grafts is limited by the immune system responses. To avoid rejection problems, autologous transplantation of the patient's corrected myoblasts has been proposed. Regr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8995

    authors： Huard C,Moisset PA,Dicaire A,Merly F,Tardif F,Asselin I,Tremblay JP

    更新日期：1998-07-30 00:00:00

  • Telomere crisis, the driving force in cancer cell evolution.

    abstract：:Cancer cells show characteristic telomere dynamics. Their chromosomes usually have short telomeres and a high telomerase activity. The "telomere crisis model" proposed here suggests that these unique telomeric features are responsible for the progression of cancer. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1006/bbrc.1996.5928

    authors： Ishikawa F

    更新日期：1997-01-03 00:00:00

  • The mitogenic response to EGF of rat hepatocytes cultured on laminin-rich gels (EHS) is blocked downstream of receptor tyrosine-phosphorylation.

    abstract：:Hepatocytes cultured on type I collagen proliferate in response to mitogenic growth factors yet de-differentiate. By contrast, hepatocytes on laminin-rich gels (EHS) demonstrate stable differentiated functions, while DNA synthesis is negligible. Here we have confirmed that this lack of proliferation was not due to det...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1647

    authors： Gardner MJ,Fletcher K,Pogson CI,Strain AJ

    更新日期：1996-11-12 00:00:00