Generation of insulin-producing cells from gnotobiotic porcine skin-derived stem cells.

abstract：:A novel inhibitor for the adhesion of monocytes to cytokine-stimulated endothelial cells, K-7174, was selected by an assay system using the cultured human monocytic cells and human endothelial cells. K-7174 inhibited the expression of vascular cell adhesion molecule-1 (VCAM-1) induced by either tumor necrosis factor a...

journal_title：Biochemical and biophysical research communications

authors： Umetani M,Nakao H,Doi T,Iwasaki A,Ohtaka M,Nagoya T,Mataki C,Hamakubo T,Kodama T

更新日期：2000-06-07 00:00:00

abstract：:(15)N and (1)HN chemical shift data and (15)N relaxation studies have been used to characterise the binding of N-phenyl-naphthylamine (NPN) to mouse major urinary protein (MUP). NPN binds in the beta-barrel cavity of MUP, hydrogen bonding to Tyr120 and making extensive non-bonded contacts with hydrophobic side chains....

journal_title：Biochemical and biophysical research communications

authors： Pertinhez TA,Ferrari E,Casali E,Patel JA,Spisni A,Smith LJ

更新日期：2009-12-25 00:00:00

abstract：:The Forkhead/Fox transcription factor Foxc2 is a critical regulator of vascular development. However, the role of Foxc2 in pathological angiogenesis in cancer remains unknown. Here we show that FoxC2 is highly expressed in human breast and colonic tumors and in the tumor endothelium in human and mouse melanomas. Using...

journal_title：Biochemical and biophysical research communications

authors： Sano H,Leboeuf JP,Novitskiy SV,Seo S,Zaja-Milatovic S,Dikov MM,Kume T

更新日期：2010-02-05 00:00:00

abstract：:Despite numerous studies on detergent-induced solubilization of membranes and on the underlying mechanisms associated with this process, very little is known regarding the selectivity of detergents for lipids during their extraction from membranes. To get insights about this phenomenon, solubilization of model bilayer...

journal_title：Biochemical and biophysical research communications

authors： Azouz M,Therrien A,Buré C,Tokarski C,Lecomte S,Lafleur M

更新日期：2020-10-15 00:00:00

abstract：:Cholesterol homeostasis is strictly maintained to prevent abnormal biological processes that arise from excessive accumulation of cholesterol in tissues. Although dyslipidemia causes reproductive dysfunction and endocrine disruption in male rats, regulatory factors and mechanisms have not been clearly demonstrated. Th...

journal_title：Biochemical and biophysical research communications

authors： Lim W,Bae H,Sohn JY,Jeong W,Kim SH,Song G

更新日期：2015-01-02 00:00:00

abstract：:The proper development of the cerebral cortex is essential for brain formation and functioning. O-GlcNAcylation, an important posttranslational modification, regulates the pathways critical for neuronal health and the survival of the cerebral cortex in neurodegenerative diseases. However, the role of O-GlcNAcylation i...

journal_title：Biochemical and biophysical research communications

authors： Cheng J,Wu Y,Chen L,Li Y,Liu F,Shao J,Huang M,Fan M,Wu H

更新日期：2020-11-19 00:00:00

abstract：:Pterostilbene, a bioactive component of blueberries and grapes, shows structural similarity to resveratrol, and exhibits antioxidant, anti-inflammatory, anti-cancer, hypoglycemic, and cholesterol lowering effects. Recent evidence indicates that pterostilbene is an agonist of the nuclear receptor, peroxisome proliferat...

journal_title：Biochemical and biophysical research communications

authors： Ren G,Rimando AM,Mathews ST

更新日期：2018-04-06 00:00:00

abstract：:We established a rapid procedure for obtaining transgenic mice by directly injecting an enhanced green fluorescent protein (EGFP)-expressing plasmid (pIRES-EGFP) into the ovaries of fertile mice. The frequency of transgenic mouse production was determined by pair-mating, and by polymerase chain reaction (PCR) and sequ...

journal_title：Biochemical and biophysical research communications

authors： Yang SY,Wang JG,Cui HX,Sun SG,Li Q,Gu L,Hong Y,Liu PP,Liu WQ

更新日期：2007-06-22 00:00:00

abstract：:Vibrio vulnificus, a halophilic estuarine bacterium causing a rapidly progressing fatal septicemia, is highly cytotoxic to eukaryotic cells. To identify new virulence factors associated with cytotoxicity, we constructed a mariner-based transposon (Tn Himar1) library of the highly virulent clinical isolate MO6-24/O hav...

journal_title：Biochemical and biophysical research communications

authors： Ran Kim Y,Haeng Rhee J

更新日期：2003-05-02 00:00:00

abstract：:Calcineurin Inhibitors (CNIs) are routinely used for immunosuppression following solid organ transplantation. However, the prolonged use of these agents lead to organ fibrosis which limits their efficacy. CNIs induce TGFβ expression, which is reported to augment endothelial-to-mesenchymal transition (EndMT), but their...

journal_title：Biochemical and biophysical research communications

authors： Woda CB,Bruneau S,Mak AL,Haskova Z,Liu K,Ghosh CC,Briscoe DM

更新日期：2019-11-19 00:00:00

abstract：:Major blood stage antimalarial drugs like chloroquine and artemisinin target the heme detoxification process of the malaria parasite. Hemozoin formation reactions in vitro using the Plasmodium falciparum histidine-rich protein-2 (Pfhrp-2), lipids, and auto-catalysis are slow and could not explain the speed of detoxifi...

journal_title：Biochemical and biophysical research communications

authors： Pandey AV,Babbarwal VK,Okoyeh JN,Joshi RM,Puri SK,Singh RL,Chauhan VS

更新日期：2003-09-05 00:00:00

abstract：:Altered brain cholesterol homeostasis plays a key role in neurodegenerative diseases such as Alzheimer's disease (AD). For a long time, the blood-brain barrier (BBB) was basically considered as a barrier isolating the brain from circulating cholesterol, however, several lines of evidence now suggest that the BBB stric...

journal_title：Biochemical and biophysical research communications

authors： Gosselet F,Saint-Pol J,Fenart L

更新日期：2014-04-11 00:00:00

abstract：:The homeodomain transcription factor Phox2b is one of the key determinants involved in the development of noradrenergic (NA) neurons in both the central nervous system (CNS) and the peripheral nervous system (PNS). Using yeast two-hybrid screening, we isolated a Phox2b interacting protein, Trim11, which belongs to TRI...

journal_title：Biochemical and biophysical research communications

authors： Hong SJ,Chae H,Lardaro T,Hong S,Kim KS

更新日期：2008-04-11 00:00:00

abstract：:AICAR (5-Aminoimidazole-4-carboxamide riboside or acadesine) is an AMP-activated protein kinase (AMPK) agonist, which induces cytotoxic effect to several cancer cells. Its potential activity in prostate cancer cells and the underlying signaling mechanisms have not been extensively studied. Here, we showed that AICAR p...

journal_title：Biochemical and biophysical research communications

authors： Guo F,Liu SQ,Gao XH,Zhang LY

更新日期：2016-05-27 00:00:00

abstract：:Lysates of rabbit reticulocytes and other mammalian cells are known to contain an activity which binds with high specificity ppp(A2'p)3A,3'-[32P]pCp. The binding activity shows a marked dependence on preincubation of lysates at different temperatures (4 degrees C - 45 degrees C). For example, binding was increased 50%...

journal_title：Biochemical and biophysical research communications

authors： Wu JM,Eslami B,Semproni AR

更新日期：1983-11-30 00:00:00

abstract：:An examination of more than 1600 protein-protein interaction sites indicated that proline is the residue most commonly found near interaction sites. A structural role is distinguished for these proline residues in the flanking segments of protein-protein interaction sites. The unique nature of proline helps protect th...

journal_title：Biochemical and biophysical research communications

authors： Kini RM,Evans HJ

更新日期：1995-07-26 00:00:00

abstract：:Temozolomide (TMZ) is a methylating agent used in chemotherapy against glioblastoma. This work was designed to clarify details in repair pathways acting to remove DNA double-strand breaks (DSBs) induced by TMZ. Cultured mouse embryonic fibroblasts were used which were deficient in DSB repair genes such as homologous r...

journal_title：Biochemical and biophysical research communications

authors： Kondo N,Takahashi A,Mori E,Ohnishi K,McKinnon PJ,Sakaki T,Nakase H,Ohnishi T

更新日期：2009-10-02 00:00:00

abstract：:Zta is a bZIP transcription factor (TF) in the Epstein-Barr virus that binds unmethylated and methylated DNA sequences. Substitution of cysteine 189 of Zta to serine (Zta(C189S)) results in a virus that is unable to execute the lytic cycle, which was attributed to a change in binding to methylated DNA sequences. To le...

journal_title：Biochemical and biophysical research communications

authors： Ray S,Tillo D,Assad N,Ufot A,Deppmann C,Durell SR,Porollo A,Vinson C

更新日期：2018-07-02 00:00:00

abstract：:Iron overload, as a risk factor for osteoporosis, can result in the up-regulation of Hepcidin, and Hepcidin knockout mice display defects in their bone microarchitecture. However, the molecular and genetic mechanisms underlying Hepcidin deficiency-derived bone loss remain unclear. Here, we show that hepcidin knockdown...

journal_title：Biochemical and biophysical research communications

authors： Jiang Y,Yan Y,Wang X,Zhu G,Xu YJ

更新日期：2016-07-15 00:00:00

abstract：:Mammalian target of rapamycin (mTOR) is a therapeutic target for head and neck squamous cell carcinoma (HNSCC). Here, we evaluated the activity of AZD-2014, a potent mTOR complex 1/2 (mTORC1/2) dual inhibitor, against HNSCC cells. We showed that AZD-2014 blocked mTORC1/2 activation in established and primary human HNS...

journal_title：Biochemical and biophysical research communications

authors： Li Y,Cui JT

更新日期：2016-09-02 00:00:00

abstract：:The effect of steroid chemical structure on steroid-lipid interaction is studied in sonicated dipalmitoyl phosphatidylcholine vesicles by steady-state and time-resolved fluorescence anisotropy techniques using diphenylhexatriene as probe. A large number of cholesterol derivatives and steroids, some of them being precu...

journal_title：Biochemical and biophysical research communications

authors： Vincent M,Gallay J

更新日期：1983-06-29 00:00:00

abstract：:Delipidated proteins from a fraction of mouse lung homogenate enriched for lamellar bodies of type 2 pneumocytes were characterized by a combination of gel filtration and enzyme immunoassay for the presence of a surfactant-associated antigen. Immunoreactivity was associated with a protein of Mr 250-270,000 as well as ...

journal_title：Biochemical and biophysical research communications

authors： Kumar RK,Smith GJ,Truscott JY,Hristoforidis CP,Lykke AW

更新日期：1986-09-30 00:00:00

abstract：:Nucleophosmin (NPM) is a multifunctional nucleolar protein that has been linked with nucleolar stress. In non-neuronal cell lines, NPM may enhance or inhibit the activity of tumor suppressor p53, a major apoptotic protein. The relationship between NPM and p53 in the central nervous system (CNS) remains unknown. Here, ...

journal_title：Biochemical and biophysical research communications

authors： Marquez-Lona EM,Tan Z,Schreiber SS

更新日期：2012-01-06 00:00:00

abstract：:Computing the ligand-protein binding affinity (or the Gibbs free energy) with chemical accuracy has long been a challenge for which many methods/approaches have been developed and refined with various successful applications. False positives and, even more harmful, false negatives have been and still are a common occu...

journal_title：Biochemical and biophysical research communications

authors： Villarreal OD,Yu L,Rodriguez RA,Chen LY

更新日期：2017-01-29 00:00:00

abstract：:Tau misfolding has been implicated in a variety of tauopathies, including Alzheimer's disease. The microtubule binding domain of tau consists of four repeat segments (R1-R4), and aggregation of these segments leads to the formation of neurofibrillary tangles. Previous studies indicate that misfolded tau associates wit...

journal_title：Biochemical and biophysical research communications

authors： Dicke SS,Tatge L,Engen PE,Culp M,Masterson LR

更新日期：2017-12-02 00:00:00

abstract：:GMP-140 (CD62; PADGEM) is a member of the selectin family expressed highly at the surface of platelets and endothelial cells by agonists such as thrombin or phorbol esters. Previous studies indicate that the lectin domain of GMP-140 recognizes sialosyl-Le(x) (SLex) and to a lesser extent Le(x) (Polley MJ, et al., Proc...

journal_title：Biochemical and biophysical research communications

authors： Handa K,Nudelman ED,Stroud MR,Shiozawa T,Hakomori S

更新日期：1991-12-31 00:00:00

abstract：:Calumenin is a multiple EF-hand Ca2+-binding protein located in endo/sarcoplasmic reticulum of mammalian tissues. In the present study, we cloned two rabbit calumenin isoforms (rabbit calumenin-1 and -2, GenBank Accession Nos. SY225335 and AY225336, respectively) by RT-PCR. Both isoforms contain a 19 aa N-terminal sig...

journal_title：Biochemical and biophysical research communications

authors： Jung DH,Mo SH,Kim DH

更新日期：2006-04-28 00:00:00

abstract：:Several rich sources of polyphenols stimulate the endothelial formation of nitric oxide (NO), a potent vasoprotecting factor, via the redox-sensitive activation of the PI3-kinase/Akt pathway leading to the phosphorylation of endothelial NO synthase (eNOS). The present study examined the molecular mechanism underlying ...

journal_title：Biochemical and biophysical research communications

authors： Auger C,Kim JH,Chabert P,Chaabi M,Anselm E,Lanciaux X,Lobstein A,Schini-Kerth VB

更新日期：2010-02-26 00:00:00

abstract：:The serine proteinase catalyzed hydrolysis of N-ethoxycarbonyl-D-phenylalanyl-L-prolyl-alpha-azalysine p- nitrophenyl ester (Eoc-D-Phe-Pro-azaLys-ONp) was investigated at pH 6.2 and 21.0 degrees C. The results are consistent with the minimum three-step catalytic mechanism. The acylation step is rate limiting for human...

journal_title：Biochemical and biophysical research communications

authors： Balliano G,Milla P,Giordano C,Gallina C,Coletta M,Menegatti E,Rizzi M,Bolognesi M,Ascenzi P

更新日期：1996-08-14 00:00:00

abstract：:The cyclin-dependent kinase (CDK) inhibitor p27(Kip1) is an important regulator of cell cycle progression as it negatively regulates G(0/1) progression and plays a major role in controlling the cell cycle. The screening of the p27(Kip1) sequence identified many potential phosphorylation sites. Although Ser(10) and Thr...

journal_title：Biochemical and biophysical research communications

authors： Guan X,Chen L,Wang J,Geng H,Chu X,Zhang Q,Du L,De W

更新日期：2006-09-01 00:00:00