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Recombinant Cry3Aa has insecticidal activity against the Andean potato weevil, Premnotrypes vorax.

Abstract：

:The Andean potato weevil, Premnotrypes vorax, an insect of the order Coleoptera, is a major cause of damage to potato crops in the Andean regions of South America. The insecticidal Cry proteins from Bacillus thuringiensis are useful biological pesticides, and some are toxic to Coleopteran insects. We overexpressed recombinant, histidine-tagged Cry3Aa protein in Escherichia coli host cells. The recombinant protein was solubilized at high pH with urea, purified using Ni(2+)-nitrilo-triacetic acid affinity resin, and dialysed to lower pH and remove urea. Bioassays were performed with an insect media whose surface was spread with 70 microgram/mL purified native or recombinant toxins. First instar larvae exposed to toxin treated media for 5 days exhibited mortalities from 57% (native Cry3Aa) to 52% (recombinant Cry3Aa). Purified native and recombinant Cry3Aa proteins appeared to be equally toxic to the Andean potato weevil.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Gomez S,Mateus AC,Hernandez J,Zimmermann BH

doi

10.1006/bbrc.2000.3998

subject

Has Abstract

pub_date

2000-12-20 00:00:00

pages

653-6

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(00)93998-6

journal_volume

279

pub_type

杂志文章

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