Abstract:
:The human poly(rC)-binding protein (PCBP) 2 is known to interact with enteroviral RNA. Here, the interaction of PCBP2 with RNA target sequences at the 5' end of the coxsackievirus B3 genome was investigated. Using the electrophoretic mobility shift assay and the yeast three-hybrid system, a short oligo(rC) tract connecting cloverleaf and IRES is demonstrated to contribute to PCBP2 binding. This oligo(rC) tract is conserved among entero- and rhinoviruses. In absence of the viral 3C proteinase, an extended cloverleaf RNA (nt 1-105) containing the oligo(rC) tract interacts with PCBP2 whereas the cloverleaf (nt 1-87) lacking the oligo(rC) tract does not. In the presence of 3C proteinase, cloverleaf RNA (1-87) interacts with PCBP2.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Zell R,Ihle Y,Seitz S,Gündel U,Wutzler P,Görlach Mdoi
10.1016/j.bbrc.2007.12.038subject
Has Abstractpub_date
2008-02-22 00:00:00pages
917-21issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(07)02645-9journal_volume
366pub_type
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