当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Functional analysis, overexpression, and kinetic characterization of pyruvate kinase from Plasmodium falciparum.

Functional analysis, overexpression, and kinetic characterization of pyruvate kinase from Plasmodium falciparum.

Abstract：

:The important role of pyruvate kinase during malarial infection has prompted the cloning of a cDNA encoding Plasmodium falciparum pyruvate kinase (pfPyrK), using mRNA from intraerythrocytic-stage malaria parasites. The full-length cDNA encodes a protein with a computed molecular weight of 55.6 kDa and an isoelectric point of 7.5. The purified recombinant pfPyrK is enzymatically active and exists as a homotetramer in its active form. The enzyme exhibits hyperbolic kinetics with respect to phosphoenolpyruvate and ADP, with K(m) of 0.19 and 0.12 mM, respectively. pfPyrK is not affected by fructose-1,6-bisphosphate, a general activating factor of pyruvate kinase for most species. Glucose-6-phosphate, an activator of the Toxoplasma gondii enzyme, does not affect pfPyrK activity. Similar to rabbit pyruvate kinase, pfPyrK is susceptible to inactivation by 1mM pyridoxal-5'-phosphate, but to a lesser extent. A screen for inhibitors to pfPyrK revealed that it is markedly inhibited by ATP and citrate. Detailed kinetic analysis revealed a transition from hyperbolic to sigmoidal kinetics for PEP in the presence of citrate, as well as competitive inhibitory behavior for ATP with respect to PEP. Citrate exhibits non-competitive inhibition with respect to ADP with a K(i) of 0.8mM. In conclusion, P. falciparum expresses an active pyruvate kinase during the intraerythrocytic-stage of its developmental cycle that may play important metabolic roles during infection.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Chan M,Sim TS

doi

10.1016/j.bbrc.2004.11.018

subject

Has Abstract

pub_date

2005-01-07 00:00:00

pages

188-96

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(04)02571-9

journal_volume

326

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • HSP70 interacts with Rheb, inhibiting mTORC1 signaling.

    abstract：:The small GTPase Rheb binds and activates mTORC1, which plays a pivotal role in diverse cellular physiologies. To increase our understanding of how Rheb regulates mTORC1 signaling, we set out to identify Rheb binding proteins using shotgun proteomics approaches. In this study, we characterized HSP70, one of the identi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.07.053

    authors： Ryu HH,Ha SH

    更新日期：2020-12-17 00:00:00

  • Identification of brain-specific angiogenesis inhibitor 2 as an interaction partner of glutaminase interacting protein.

    abstract：:The vast majority of physiological processes in living cells are mediated by protein-protein interactions often specified by particular protein sequence motifs. PDZ domains, composed of 80-100 amino acid residues, are an important class of interaction motif. Among the PDZ-containing proteins, glutaminase interacting p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.07.029

    authors： Zencir S,Ovee M,Dobson MJ,Banerjee M,Topcu Z,Mohanty S

    更新日期：2011-08-12 00:00:00

  • Identification of miR-125b targets involved in acute promyelocytic leukemia cell proliferation.

    abstract：:Acute promyelocytic leukemia (APL) is characterized by the presence of the PML-RARα fusion protein. We have previously found that PML-RARα-regulated miR-125b is highly expressed in APL; however, the characteristics of the regulatory effects and mechanisms of miR-125b involved in APL proliferation have yet to be clarif...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.09.020

    authors： Zhang Y,Zeng C,Lu S,Qin T,Yang L,Chen S,Chen J,Li Y

    更新日期：2016-09-30 00:00:00

  • Expression of Smads during in vitro transdifferentiation of hepatic stellate cells to myofibroblasts.

    abstract：:TGFbeta is of crucial importance during transdifferentiation of resting retinoid-storing hepatic stellate cells (HSC) to extracellular matrix producing myofibroblasts (MFB) and consequently, inhibition of TGFbeta signal transduction is an effective means for preventing experimental fibrosis. We have shown that isolate...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4811

    authors： Dooley S,Streckert M,Delvoux B,Gressner AM

    更新日期：2001-05-11 00:00:00

  • Monoclonal antibody-directed immunopurification and identification of cytochromes P-450.

    abstract：:Several rat liver cytochromes P-450 have been substantially purified in a one-step immunoadsorption procedure using Sepharose-bound monoclonal antibodies (MAbs) to the major forms of rat liver cytochrome P-450 induced by 3-methylcholanthrene and phenobarbital (MC-P-450 and PB-P-450, respectively). When mixed with solu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(83)80221-6

    authors： Friedman FK,Robinson RC,Park SS,Gelboin HV

    更新日期：1983-11-15 00:00:00

  • Chlamydia trachomatis and Chlamydia pneumoniae bind specifically to phosphatidylethanolamine in HeLa cells and to GalNAc beta 1-4Gal beta 1-4GLC sequences-found in asialo-GM1 and asial-GM2.

    abstract：:To examine the possible role of lipids as adhesion receptors for infection, Chlamydia trachomatis and Chlamydia pneumoniae were labeled with 125I and layered on thin-layer chromatograms (tlc) of separated lipids isolated from target cells, and bound bacteria were detected by autoradiography. Elementary bodies from bot...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91676-4

    authors： Krivan HC,Nilsson B,Lingwood CA,Ryu H

    更新日期：1991-03-29 00:00:00

  • Endothelin-3 is a novel neuropeptide: isolation and sequence determination of endothelin-1 and endothelin-3 in porcine brain.

    abstract：:The molecular forms of endothelin (ET) related peptides were investigated in porcine brain by using high performance liquid chromatography coupled with three specific radioimmunoassays. ET-1 and its oxidized form were isolated and sequenced as in the case of porcine spinal cord. A very small amount of big ET-1 (1-39) ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91760-9

    authors： Shinmi O,Kimura S,Sawamura T,Sugita Y,Yoshizawa T,Uchiyama Y,Yanagisawa M,Goto K,Masaki T,Kanazawa I

    更新日期：1989-10-16 00:00:00

  • A hypothetical structural role for proline residues in the flanking segments of protein-protein interaction sites.

    abstract：:An examination of more than 1600 protein-protein interaction sites indicated that proline is the residue most commonly found near interaction sites. A structural role is distinguished for these proline residues in the flanking segments of protein-protein interaction sites. The unique nature of proline helps protect th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2084

    authors： Kini RM,Evans HJ

    更新日期：1995-07-26 00:00:00

  • Structural insight into a novel indole prenyltransferase in hapalindole-type alkaloid biosynthesis.

    abstract：:FamD1 is a novel CloQ/NphB-family indole prenyltransferase which involves in hapalindole-type alkaloid biosynthesis. Here the native FamD1 structure and three protein-ligand complexes are analyzed to investigate the molecular basis of substrate binding and catalysis. FamD1 adopts a typical ABBA architecture of aromati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.12.039

    authors： Wang J,Chen CC,Yang Y,Liu W,Ko TP,Shang N,Hu X,Xie Y,Huang JW,Zhang Y,Guo RT

    更新日期：2018-01-08 00:00:00

  • Phosphorylation of A170 stress protein by casein kinase II-like activity in macrophages.

    abstract：:A170 is an oxidative stress-inducible protein having a Zinc finger domain, two PEST sequences, and many potential phosphorylation sites for serine/threonine kinases. These structural features suggest that the phosphorylation of A170 affects its function and degradation. We have found that A170 is phosphorylated in cul...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7783

    authors： Yanagawa T,Yuki K,Yoshida H,Bannai S,Ishii T

    更新日期：1997-12-08 00:00:00

  • Leukotriene signaling via ALOX5 and cysteinyl leukotriene receptor 1 is dispensable for in vitro growth of CD34+CD38- stem and progenitor cells in chronic myeloid leukemia.

    abstract：:Tyrosine kinase inhibitors targeting the BCR-ABL oncoprotein in chronic myeloid leukemia (CML) are remarkably effective inducing deep molecular remission in most patients. However, they are less effective to eradicate the leukemic stem cells (LSC), resulting in disease persistence. Therefore, there is great need to de...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.06.051

    authors： Dolinska M,Piccini A,Wong WM,Gelali E,Johansson AS,Klang J,Xiao P,Yektaei-Karin E,Strömberg UO,Mustjoki S,Stenke L,Ekblom M,Qian H

    更新日期：2017-08-19 00:00:00

  • Oxidized low density lipoprotein suppresses lipopolysaccharide-induced inflammatory responses in microglia: oxidative stress acts through control of inflammation.

    abstract：:Low density lipoprotein (LDL) is readily oxidized under certain conditions, resulting in the formation of oxidized LDL (oxLDL). Despite numerous in vitro reports that reveal the pathogenic role of oxidative stress, anti-oxidative strategies have underperformed in the clinic. In this study, we examine the role of oxLDL...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.01.107

    authors： Kim OS,Lee CS,Joe EH,Jou I

    更新日期：2006-03-31 00:00:00

  • Ambroxol-induced modification of ion transport in human airway Calu-3 epithelia.

    abstract：:Ambroxol is often used as a mucolytic agent in various lung diseases. However, it is unclear how ambroxol acts on bronchial epithelial cells. To clarify the action of ambroxol, we studied the effects of ambroxol on the ion transport in human Calu-3 cells, a human submucosal serous cell line, measuring the transepithel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.03.009

    authors： Hasegawa I,Niisato N,Iwasaki Y,Marunaka Y

    更新日期：2006-05-05 00:00:00

  • Tumor necrosis factor alpha receptor p55 is under hormonal (follicle-stimulating hormone) control in testicular Sertoli cells.

    abstract：:Identification of TNF alpha receptors and their regulation by the endocrine system were examined in testicular Sertoli cells. By using cross-linking experiments as well as northern blotting analysis, we detected the p55 receptor type (MW: 55 kDa, mRNA: 2.3 kb), but not the p75 TNF alpha receptor type. Furthermore, we ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1077

    authors： Mauduit C,Besset V,Caussanel V,Benahmed M

    更新日期：1996-07-25 00:00:00

  • Comparative study of collagen and gelatin in chitosan-based hydrogels for effective wound dressing: Physical properties and fibroblastic cell behavior.

    abstract：:The influence of collagen as an effective substitute for gelatin was investigated on properties of chitosan/gelatin hydrogels for fibroblasts growth and attachment for wound dressing applications. We synthesized hydrogels based on chitosan associated with collagen and gelatin biopolymers (in the ratio of 1:5 and 1:1, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.08.102

    authors： Mousavi S,Khoshfetrat AB,Khatami N,Ahmadian M,Rahbarghazi R

    更新日期：2019-10-22 00:00:00

  • Ionic interaction of the HIV-1 V3 domain with CCR5 and deregulation of T lymphocyte function.

    abstract：:We have reported that the principal neutralizing domain of V3 of the HIV-1 gp120 induces an antigen-specific activation apoptosis of responding effector CD4+ T lymphocytes, a phenomenon inhibited by RANTES, an agonist of CCR5. Here, addressing the question of how a hypervariable region could induce such a selective re...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02511-1

    authors： Baritaki S,Zafiropoulos A,Sioumpara M,Politis M,Spandidos DA,Krambovitis E

    更新日期：2002-11-08 00:00:00

  • STAT1-induced upregulation of LINC00467 promotes the proliferation migration of lung adenocarcinoma cells by epigenetically silencing DKK1 to activate Wnt/β-catenin signaling pathway.

    abstract：:Long non-coding RNAs (lncRNAs) have been reported as essential regulators in human cancers, including lung adenocarcinoma (LUAD). In the present study, we found that lncRNA long intergenic non-protein coding RNA 467 (LINC00467) was expressed higher in TCGA LUAD samples and predicted poor prognosis in LUAD patients. Hi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.04.107

    authors： Yang J,Liu Y,Mai X,Lu S,Jin L,Tai X

    更新日期：2019-06-18 00:00:00

  • Loading alters actin dynamics and up-regulates cofilin gene expression in chondrocytes.

    abstract：:Chondrocyte mechanotransduction in response to mechanical loading is essential for the health and homeostasis of articular cartilage. The actin cytoskeleton has been implicated in cell mechanics and mechanotransduction. This study tests the hypothesis that loading modulates actin dynamics and organisation with subsequ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.06.185

    authors： Campbell JJ,Blain EJ,Chowdhury TT,Knight MM

    更新日期：2007-09-21 00:00:00

  • Aging-associated increase of gelsolin for apoptosis resistance.

    abstract：:Gelsolin, a Ca(2+)-dependent actin regulatory protein, was recently suggested to participate in apoptosis regulation. In this study, we found that the level of gelsolin is elevated in senescent human diploid fibroblasts (HDFs) and also in the tissues of old rats, i.e., in the liver, kidney, heart, spleen, stomach, and...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.11.061

    authors： Ahn JS,Jang IS,Kim DI,Cho KA,Park YH,Kim K,Kwak CS,Chul Park S

    更新日期：2003-12-26 00:00:00

  • Localization of a negative vitamin D response sequence in the human growth hormone gene.

    abstract：:1,25-Dihydroxyvitamin D(3) [1,25(OH)(2) D(3)] exerts its biological effects by binding to the vitamin D receptor (VDR), which binds in turn to the vitamin D response elements located in the target gene's promoter. We have previously demonstrated that VDR binds in vitro with high affinity to the 5'-flanking sequence of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6641

    authors： Seoane S,Alonso M,Segura C,Pérez-Fernández R

    更新日期：2002-03-22 00:00:00

  • Active fragments and analogs of the insect neuropeptide leucopyrokinin: structure-function studies.

    abstract：:Evaluation of analogs of the blocked insect myotropic neuropeptide leucopyrokinin (LPK) has demonstrated its relative insensitivity to amino acid substitution in the N-terminal in contrast to the C-terminal region. Truncated analogs of LPK without the first, second, and third N-terminal amino acids retain a significan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90315-3

    authors： Nachman RJ,Holman GM,Cook BJ

    更新日期：1986-06-30 00:00:00

  • Effects of colupulone, a component of hops and brewers yeast, and chromium on glucose tolerance and hepatic cytochrome P450 in nondiabetic and spontaneously diabetic mice.

    abstract：:Brewers yeast contains factors that increase and decrease glucose tolerance. Hop components (lupulones) that adhere to yeast during the brewing process elicit a variety of biological effects including the induction of hepatic cytochrome P4503A. Colupulone was tested for its effects on glucose tolerance and cytochrome ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1614

    authors： Mannering GJ,Shoeman JA,Shoeman DW

    更新日期：1994-05-16 00:00:00

  • Human MCRS2, a cell-cycle-dependent protein, associates with LPTS/PinX1 and reduces the telomere length.

    abstract：:Human LPTS/PinX1 is a telomerase-inhibitory protein, which binds to the telomere protein Pin2/TRF1 and the catalytic subunit hTERT of telomerase. To explore the proteins that might be involved in the telomerase pathway, we performed a yeast two-hybrid screening with LPTS/PinX1 as the bait. A novel gene, MCRS2, encodin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.02.166

    authors： Song H,Li Y,Chen G,Xing Z,Zhao J,Yokoyama KK,Li T,Zhao M

    更新日期：2004-04-16 00:00:00

  • ADP-ribosylation of wild-type p53 in vitro: binding of p53 protein to specific p53 consensus sequence prevents its modification.

    abstract：:We have recently reported that mutant but not wild-type (wt) p53 protein was ADP-ribosylated in primary rat cells overexpressing the temperature-sensitive murine p53val135 gene. To examine whether the lack of susceptibility to modification is a specific feature of p53val135 adopting wt conformation or rather a general...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0990

    authors： Wesierska-Gadek J,Schmid G,Cerni C

    更新日期：1996-07-05 00:00:00

  • Role of insulin-like growth factor-1 (IGF-1) in regulating cell cycle progression.

    abstract：AIMS:Insulin-like growth factor-1 (IGF-1) is a polypeptide protein hormone, similar in molecular structure to insulin, which plays an important role in cell migration, cell cycle progression, cell survival and proliferation. In this study, we investigated the possible mechanisms of IGF-1 mediated cell cycle redistribut...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.08.114

    authors： Ma QL,Yang TL,Yin JY,Peng ZY,Yu M,Liu ZQ,Chen FP

    更新日期：2009-11-06 00:00:00

  • Rpg1p/Tif32p, a subunit of translation initiation factor 3, interacts with actin-associated protein Sla2p.

    abstract：:The yeast two-hybrid system was used to screen for proteins that interact in vivo with Saccharomyces cerevisiae Rpg1p/Tif32p, the large subunit of the translation initiation factor 3 core complex (eIF3). Eight positive clones encoding portions of the SLA2/END4/MOP2 gene were isolated. They overlapped in the region of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4721

    authors： Palecek J,Hasek J,Ruis H

    更新日期：2001-04-20 00:00:00

  • Base-pair opening dynamics of the microRNA precursor pri-miR156a affect temperature-responsive flowering in Arabidopsis.

    abstract：:Internal and environmental cues, including ambient temperature changes, regulate the timing of flowering in plants. Arabidopsis miR156 represses flowering and plays an important role in the regulation of temperature-responsive flowering. However, the molecular basis of miR156 processing at lower temperatures remains l...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.01.185

    authors： Kim HE,Kim W,Lee AR,Jin S,Jun AR,Kim NK,Lee JH,Ahn JH

    更新日期：2017-03-18 00:00:00

  • Multiplex standardized RT-PCR for expression analysis of many genes in small samples.

    abstract：:Standardized RT-PCR (StaRT-PCR) enables numerical quantification as well as intra- and inter-laboratory comparison of gene expression. Multiplex StaRT-PCR, using two rounds of amplification, was conducted on Stratagene Universal Reference RNA. In the first round, cDNA, competitive template (CT) mix, and primers for up...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/S0006-291X(02)00243-7

    authors： Crawford EL,Warner KA,Khuder SA,Zahorchak RJ,Willey JC

    更新日期：2002-04-26 00:00:00

  • Genomic analyses and expression evaluation of thaumatin-like gene family in the cacao fungal pathogen Moniliophthora perniciosa.

    abstract：:Thaumatin-like proteins (TLPs) are found in diverse eukaryotes. Plant TLPs, known as Pathogenicity Related Protein (PR-5), are considered fungal inhibitors. However, genes encoding TLPs are frequently found in fungal genomes. In this work, we have identified that Moniliophthora perniciosa, a basidiomycete pathogen tha...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.09.054

    authors： Franco Sde F,Baroni RM,Carazzolle MF,Teixeira PJ,Reis O,Pereira GA,Mondego JM

    更新日期：2015-10-30 00:00:00

  • Quercetin down-regulates signal transduction in human breast carcinoma cells.

    abstract：:Signal transduction activity was markedly elevated in cancer cells as shown by the increased activity of enzymes utilizing 1-phosphatidylinositol, PI (PI 4-kinase and PI-4-phosphate 5-kinase) for the production of the second messenger inositol 1,4,5-trisphosphate, IP3, in rat hepatomas (Cancer Res. 54: 2611;5574, 1994...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1355

    authors： Singhal RL,Yeh YA,Praja N,Olah E,Sledge GW Jr,Weber G

    更新日期：1995-03-08 00:00:00