Chemical modification of the glycine receptor with fluorescein isothiocyanate specifically affects the interaction of glycine with its binding site.

abstract：:The human kallikrein family is a group of 15 serine protease genes clustered on chromosome 19q13.4 and shares a high degree of homology. These proteolytic enzymes have diverse physiological functions in many different tissues. Growing evidence suggests that many kallikreins are differentially expressed in cancer and m...

journal_title：Biochemical and biophysical research communications

authors： Kapadia C,Ghosh MC,Grass L,Diamandis EP

更新日期：2004-10-22 00:00:00

abstract：:trans-Resveratrol (3,4',5-trihydroxystilbene; RES), a polyphenol found in particularly high concentrations in red wine, has recently attracted intense interest for its potentially beneficial effects on human health. Here, we report the effects of long-term exposure to micromolar concentrations of RES on antioxidant an...

journal_title：Biochemical and biophysical research communications

authors： Robb EL,Page MM,Wiens BE,Stuart JA

更新日期：2008-03-07 00:00:00

abstract：:The promoter of the GATA-4 gene was analyzed in P19CL6 cells. A 124bp segment containing conserved two GC-boxes and E-box was essential for the basal promoter activity, as determined with a transient luciferase reporter gene assay. However, an extended 1312 bp reporter construct but not the 124 bp segment, when ligate...

journal_title：Biochemical and biophysical research communications

authors： Ishibashi T,Yokura Y,Ohashi K,Yamamoto H,Maeda M

更新日期：2011-09-23 00:00:00

abstract：:X chromosome inactivation in mammalian females occurs early in embryonic development and renders most genes on the inactive X chromosome transcriptionally silenced. As a consequence, females will display an X chromosomal parent-of-origin mosaiscism with regard to which parental allele that is expressed. Some genes, ho...

journal_title：Biochemical and biophysical research communications

authors： Nordquist N,Oreland L

更新日期：2006-09-22 00:00:00

abstract：:Incorporation of [14C]serine into phosphatidylserine by the base exchange reaction in Ehrlich ascites tumor cells is inhibited by 30% by glucose and by 60% by 2-deoxyglucose (10 mM each). The inhibition by thapsigargin (0.2 microM) amounts to 80%. This inhibition is interpreted as being due to depletion of calcium sto...

journal_title：Biochemical and biophysical research communications

authors： Rakowska M,Wojtczak L

更新日期：1995-02-06 00:00:00

abstract：:Recently, T cell cytokines such as IL-17 and IFN-γ have been shown to play important roles in the progression of brain injury induced by ischemia. We have shown that IL-23 from infiltrated macrophages activates γδT cells, thereby inducing IL-17 from these cells. However, deletion of the IL-23 gene in mice showed a mor...

journal_title：Biochemical and biophysical research communications

authors： Konoeda F,Shichita T,Yoshida H,Sugiyama Y,Muto G,Hasegawa E,Morita R,Suzuki N,Yoshimura A

更新日期：2010-11-19 00:00:00

abstract：:Adiponectin, an adipocyte-derived hormone, reportedly suppresses the production of TNF-alpha and IL-6 by LPS-stimulated human or porcine macrophages, and the phagocytosis of microbeads by human macrophages. In this study, we used a high molecular weight form of adiponectin purified from human plasma to examine its eff...

journal_title：Biochemical and biophysical research communications

authors： Saijo S,Nagata K,Nakano Y,Tobe T,Kobayashi Y

更新日期：2005-09-09 00:00:00

abstract：:Hepatic stellate cells (HSCs) are major players in liver fibrogenesis. Accumulating evidence shows that suppression of autophagy plays an important role in the development and progression of liver disease. Phospholipase D1 (PLD1), which catalyzes the hydrolysis of phosphatidylcholine to yield phosphatidic acid (PA) an...

journal_title：Biochemical and biophysical research communications

authors： Seo HY,Jang BK,Jung YA,Lee EJ,Kim HS,Jeon JH,Kim JG,Lee IK,Kim MK,Park KG

更新日期：2014-06-20 00:00:00

abstract：:Adenovirus vector is one of the most widely used vectors in gene therapy applications for the treatment of diverse human diseases including cancer. In this study, we showed that infection with E1E3-deleted recombinant human adenovirus serotype 5 reduced human telomerase reverse transcriptase (hTERT) mRNA levels in hep...

journal_title：Biochemical and biophysical research communications

authors： Lee CH,Kim HW,Kim T,Lee SW

更新日期：2015-03-20 00:00:00

abstract：:At 10 microM, 1-0-oleoyl-, 1-0-palmitoyl-, and 1-0-myristoyl-2-0-acetyl-glycerol weakly stimulated neutrophils to release lysozyme, an enzyme in secondary granules, but had no such effect on the release of a primary granule enzyme, beta-glucuronidase. The glycerides (1-10 microM) had a second effect on both granule po...

journal_title：Biochemical and biophysical research communications

authors： O'Flaherty JT,Schmitt JD,McCall CE,Wykle RL

更新日期：1984-08-30 00:00:00

abstract：:We have previously shown that estrogen effects in the heart include direct hormone effects on the myocardium. In a recent study we found that one beneficial effect of estradiol on the myocardium is the inhibition of apoptosis in cardiac myocytes. This effect was associated with a reduction of NF-kappaB activity. In th...

journal_title：Biochemical and biophysical research communications

authors： Pelzer T,Neumann M,de Jager T,Jazbutyte V,Neyses L

更新日期：2001-09-07 00:00:00

abstract：:Rat neuromedin U was isolated from the small intestine using mainly immunoaffinity chromatography and radioimmunoassay for pig neuromedin U-8. The amino acid sequence of rat neuromedin U was determined by microsequence analysis to be Tyr-Lys-Val-Asn-Glu-Tyr-Gln-Gly-Pro-Val-Ala-Pro-Ser-Gly-Gly- Phe-Phe-Leu-Phe-Arg-Pro-...

journal_title：Biochemical and biophysical research communications

authors： Minamino N,Kangawa K,Honzawa M,Matsuo H

更新日期：1988-10-14 00:00:00

abstract：:We earlier reported the mitogenic and immunostimulatory activities of Rhizoctonia bataticola lectin (RBL), purified from phytopathogenic fungus R. bataticola in human PBMC. The lectin demonstrates specificity towards glycoproteins containing complex N-glycans. Since CD45-protein tyrosine phosphatase that abundantly ex...

journal_title：Biochemical and biophysical research communications

authors： Pujari R,Eligar SM,Kumar N,Nagre NN,Inamdar SR,Swamy BM,Shastry P

更新日期：2012-03-23 00:00:00

abstract：:Dysregulation and aggregation of the peptide hormone IAPP (islet amyloid polypeptide, a.k.a. amylin) into soluble oligomers that appear to be cell-toxic is a known aspect of diabetes mellitus (DM) Type 2 pathology. IAPP aggregation is influenced by several factors including interactions with metal ions such as Cu(II)....

journal_title：Biochemical and biophysical research communications

authors： Dong X,Svantesson T,Sholts SB,Wallin C,Jarvet J,Gräslund A,Wärmländer SKTS

更新日期：2019-03-19 00:00:00

abstract：:Flowers have distinct glycerolipid composition, yet its floral organ-specific profile remains elusive in Arabidopsis whose flowers are too tiny to dissect different floral organs. Here, we employed known floral homeotic mutants agamous-1 (ag-1) and apetala3-3 (ap3-3) to facilitate sample preparation enriched in differ...

journal_title：Biochemical and biophysical research communications

authors： Nakamura Y,Liu YC,Lin YC

更新日期：2014-08-08 00:00:00

abstract：:Protein kinase D (PKD) has been established as a negative modulator of the c-Jun N-terminal kinase (JNK) signaling pathway. We previously demonstrated that induced expression of constitutively active PKD (PKD-S744/748E) that mimics phosphorylation by PKC is sufficient to attenuate epidermal growth factor (EGF) stimula...

journal_title：Biochemical and biophysical research communications

authors： Hurd C,Rozengurt E

更新日期：2003-03-21 00:00:00

abstract：:ERK5, a member of the mitogen activated protein kinase, expressed in the kidneys was smaller (∼80kDa) in apparent molecular mass compared to other organs (∼120kDa). A blocking peptide experiment confirmed that the ∼80kDa detected on Western blots was a specific band detected by the anti-ERK5 antibody. Expression of th...

journal_title：Biochemical and biophysical research communications

authors： Kawakami T,Park SW,Kaku R,Yang J

更新日期：2012-02-24 00:00:00

abstract：:A radioactive photoaffinity probe for the insulin receptor was prepared by derivatizing insulin at its B29 lysine with a novel crosslinking reagent having a cleavable azo linkage. Insulin receptors purified from human placental membranes were photoaffinity labeled with this probe. The photolabeled receptor was treated...

journal_title：Biochemical and biophysical research communications

authors： Ng DS,Yip CC

更新日期：1985-11-27 00:00:00

abstract：:We describe the fusion of enhanced green fluorescent protein to the C-terminus of the HsdS DNA sequence-specificity subunit of the Type I DNA modification methyltransferase M.EcoKI. The fusion expresses well in vivo and assembles with the two HsdM modification subunits. The fusion protein functions as a sequence-speci...

journal_title：Biochemical and biophysical research communications

authors： Chen K,Roberts GA,Stephanou AS,Cooper LP,White JH,Dryden DT

更新日期：2010-07-23 00:00:00

abstract：:Toxoplasmosis is a worldwide disease caused by Toxoplasma gondii. Activated macrophages control T. gondii growth by nitric oxide (NO) production. However, T. gondii active invasion inhibits NO production, allowing parasite persistence. Here we show that the mechanism used by T. gondii to inhibit NO production persisti...

journal_title：Biochemical and biophysical research communications

authors： Seabra SH,de Souza W,Damatta RA

更新日期：2004-11-12 00:00:00

abstract：:Lateral diffusion coefficients (D) of two surface differentiation antigens (sIgM and Bp35) were determined on interferon-sensitive (-IFs) or resistant (-IFr) Daudi cells by fluorescence photobleaching, using monospecific FITC-anti-IgM or PE-anti-Leu 16 probes. For untreated Daudi -IFs, mean (D) were 5.8 and 5.3 (x10(-...

journal_title：Biochemical and biophysical research communications

authors： Balint E,Aszalos A,Grimley PM

更新日期：1988-12-15 00:00:00

abstract：:The apical membrane of distal nephron epithelium (A6) had a Ca(2+)-activated outwardly rectifying Cl- channel with single-channel conductances of 3 pS for outward current and 0.8 pS for inward current. The single-channel conductance for inward current was dependent on cytosolic Ca2+ concentration. Insulin increased th...

journal_title：Biochemical and biophysical research communications

authors： Shintani Y,Marunaka Y

更新日期：1996-01-05 00:00:00

abstract：:Transmissible spongiform encephalopathies are fatal neurodegenerative disorders thought to be transmitted by self-perpetuating conformational conversion of a neuronal membrane glycoprotein (PrP(C), for "cellular prion protein") into an abnormal state (PrP(Sc), for "scrapie prion protein"). Doppel (Dpl) is a protein th...

journal_title：Biochemical and biophysical research communications

authors： Erlich P,Cesbron JY,Lemaire-Vieille C,Curt A,Andrieu JP,Schoehn G,Jamin M,Gagnon J

更新日期：2008-01-18 00:00:00

abstract：:The formation of oxidized low density lipoprotein (LDL) is believed to play a significant role in the pathogenesis of atherosclerosis. Myoglobin in the presence of H(2)O(2) has been shown to catalyze LDL oxidation in vitro. It is established that an oxidatively altered form of myoglobin (Mb-H), which contains a prosth...

journal_title：Biochemical and biophysical research communications

authors： Vuletich JL,Osawa Y,Aviram M

更新日期：2000-03-24 00:00:00

abstract：:We report the usefulness of an impedance-based label-free whole cell assay to identify new ligands for G protein-coupled receptors (GPCRs) involved in microglial cell migration. Authentic GPCR ligands were subjected to the impedance-based cell assay in order to examine the responses of ligands for MG5 mouse microglial...

journal_title：Biochemical and biophysical research communications

authors： Fukano Y,Okino N,Furuya S,Ito M

更新日期：2016-09-16 00:00:00

abstract：:2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and related compounds modulate various endocrine functions by enhancing ligand metabolism, altering hormone synthesis, down regulating receptor levels, and interfering with gene transcription. In the present study, we investigated the effects of TCDD on testosterone signal tr...

journal_title：Biochemical and biophysical research communications

authors： Jana NR,Sarkar S,Ishizuka M,Yonemoto J,Tohyama C,Sone H

更新日期：1999-03-24 00:00:00

abstract：:The (45)Ca(2+) influx into right-side-out resealed ghosts (RG) prepared from human red blood cells (RBC) was measured. The (45)Ca(2+) equilibration occurred with t(1/2)=2.5 min and the steady-state was reached after 17 min with the level of 22+/-2 micromol/L(packed cells) at 37 degrees C. The rate of the influx was 97...

journal_title：Biochemical and biophysical research communications

authors： Hudec R,Lakatos B,Orlický J,Varecka L

更新日期：2004-12-24 00:00:00

abstract：:Recently a rabbit cDNA (F3R) was characterized as binding and causing calcium mobilization induced by the formyl-methionine-leucine-phenylalanine peptide (fMLP). In the study reported here, cloned DNAs were isolated from rabbit genomic DNA by PCR based on the sequence of F3R. The cloned DNAs have several differences i...

journal_title：Biochemical and biophysical research communications

authors： Beckmann MP,Munger WE,Kozlosky C,VandenBos T,Price V,Lyman S,Gerard NP,Gerard C,Cerretti DP

更新日期：1991-09-16 00:00:00

abstract：:Cardiac ventricular myocytes exhibit a protein kinase A-dependent Cl(-) current (ICl.PKA) mediated by the cystic fibrosis transmembrane conductance regulator (CFTR). There is conflicting evidence regarding the ability of the divalent cation nickel (Ni(2+)), which has been used widely in vitro in the study of other car...

journal_title：Biochemical and biophysical research communications

authors： Barman PP,Cheng H,Hancox JC,James AF

更新日期：2013-03-01 00:00:00

abstract：:A histidine-tagged recombinant N-terminal fragment of type-1 mouse liver diacylglycerol acyltransferase (DGAT; EC 2.3.1.20), MmDGAT1(1-95)His6, was expressed in Escherichia coli, and used to investigate possible acyl-CoA-binding properties. Analysis of the purified fragment by MALDI-TOF mass spectrometry revealed a po...

journal_title：Biochemical and biophysical research communications

authors： Siloto RM,Madhavji M,Wiehler WB,Burton TL,Boora PS,Laroche A,Weselake RJ

更新日期：2008-08-29 00:00:00