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Separation and partial characterization of soluble fatty acid alpha-hydroxylase from Sphingomonas paucimobilus.

Abstract：

:The crude extracts from Sphingomonas paucimobilus containing 2-hydroxy fatty acid-rich sphingolipids were found to oxidize [1-14C]-myristate to 2-hydroxymyristate in the presence of NADH. The myristate-oxidation activity was partially purified about 290-fold from the cell-free extracts by ammonium sulfate fractionation and hydroxylapatite chromatography. When laurate, myristate, and palmitate were used as the substrates, the reaction products were identified as the corresponding 2-hydroxy fatty acids by gas-chromatography (GC), HPLC, and GC-mass spectrometry. The enzyme preparation required NADH and molecular oxygen for its alpha-hydroxylation activity, suggesting that the bacterial alpha-hydroxylation is typical of monooxygenase reactions. Of fatty acids tested, myristate was the most efficient substrate.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Matsunaga I,Kusunose E,Yano I,Ichihara K

doi

10.1006/bbrc.1994.1881

subject

Has Abstract

pub_date

1994-06-30 00:00:00

pages

1554-60

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(84)71881-X

journal_volume

201

pub_type

杂志文章

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