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RNA mutations of prox1 detected in human esophageal cancer cells by the shifted termination assay.

Abstract：

:We have recently reported a novel finding that a candidate tumor suppressor gene prox1 suffered adenosine-to-inosine (A-to-I) RNA mutation without genomic mutation in a subset of human cancer cells and lost its function. Hence, screening of mutations in both cDNA and genomic DNA could be important in the analysis of causes for cancers. Here, we applied a sensitive, accurate, and simple method, called shifted termination assay (STA) for detection of an A-to-I RNA mutation (R334G) in prox1. We prepared PCR-amplified samples containing the target base of RNA mutation from cDNAs and genomic DNAs of various cell lines and clinical samples, to demonstrate that the STA method can be used to identify not only genomic mutations but also RNA mutations more effectively compared to sequencing. By means of STA, we found prox1 R334G RNA mutations but not genomic DNA mutations in 4 of 8 cases of esophageal cancers. This method can help us to detect RNA mutation effectively and progress research of a potential oncogenic principle.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Yoshimoto T,Takahashi M,Nagayama S,Watanabe G,Shimada Y,Sakasi Y,Kubo H

doi

10.1016/j.bbrc.2007.05.071

subject

Has Abstract

pub_date

2007-07-27 00:00:00

pages

258-62

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)01032-7

journal_volume

359

pub_type

杂志文章

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