Reduced expression of Tis7/IFRD1 protein in murine and human cystic fibrosis airway epithelial cell models homozygous for the F508del-CFTR mutation.

Abstract:

:12-O-tetradecanoyl phorbol-13-acetate-induced sequence 7/interferon related development regulator 1 (Tis7/IFRD1) has been recently identified as a modifier gene in lung inflammatory disease severity in patients with cystic fibrosis (CF), based upon its capacity to regulate inflammatory activities in neutrophils. In CF patients, the F508del mutation in the Cftr gene encoding a chloride channel, the CF transmembrane conductance regulator (CFTR) in airway epithelial cells results in an exaggerated inflammatory response of these cells. At present, it is unknown whether the Tis7/IFRD1 gene product is expressed in airway epithelial cells. We therefore investigated the possibility there is an intrinsic alteration in Tis7/IFRD1 protein level in cells lacking CFTR function in tracheal homogenates of F508del-CFTR mice and in a F508del-CFTR human bronchial epithelial cell line (CFBE41o(-) cells). When Tis7/IFRD1 protein was detectable, trachea from F508del-CFTR mice showed a reduction in the level of Tis7/IFRD1 protein compared to wild-type control littermates. A significant reduction of IFRD1 protein level was found in CFBE41o(-) cells compared to normal bronchial epithelial cells 16HBE14o(-). Surprisingly, messenger RNA level of IFRD1 in CFBE41o(-) cells was found elevated. Treating CFBE41o(-) cells with the antioxidant glutathione rescued the IFRD1 protein level closer to control level and also reduced the pro-inflammatory cytokine IL-8 release. This work provides evidence for the first time of reduced level of IFRD1 protein in murine and human F508del-CFTR airway epithelial cell models, possibly mediated in response to oxidative stress which might contribute to the exaggerated inflammatory airway response observed in CF patients homozygous for the F508del mutation.

authors

Blanchard E,Marie S,Riffault L,Bonora M,Tabary O,Clement A,Jacquot J

doi

10.1016/j.bbrc.2011.06.104

subject

Has Abstract

pub_date

2011-08-05 00:00:00

pages

471-6

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(11)01084-9

journal_volume

411

pub_type

杂志文章
  • Amylase secretion from isolated pure acinar cells.

    abstract::Isolation of pure acinar cells of the rat pancreas was achieved employing counterflow sedimentation filtration technique (CSFT). The preparation of purified acinar cells contained an occasional red blood cell (RBC, 200:1) with total absence of endocrine and duct cells. A significant stimulation of amylase secretion fr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(85)91403-2

    authors: Bandisode MS,Singh M

    更新日期:1985-05-31 00:00:00

  • Long noncoding RNA UCA1 promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells via miRNA-145-5p/SMAD5 and miRNA-124-3p/SMAD4 axis.

    abstract::Long noncoding RNA (lncRNAs) UCA1 has been known to be critical for the chondrogenic differentiation of marrow mesenchymal stem cells (MSCs). In this study, we explore the effects and mechanisms of UCA1 on the promotion of chondrogenesis of MSCs. During the processes of chondrogenic differentiation of MSCs, UCA1, miRN...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.04.140

    authors: Shu T,He L,Wang X,Pang M,Yang B,Feng F,Wu Z,Liu C,Zhang S,Liu B,Wang Q,Rong L

    更新日期:2019-06-18 00:00:00

  • Alpha-shaped DNA loops induced by MutS.

    abstract::DNA mismatch repair (MMR) is critical for the maintenance of genomic stability. MMR is initiated by recognition of DNA mismatches by the protein, MutS, which subsequently recruits downstream repair factors. To better understand the mechanism by which MutS identifies and specifically binds mismatched basepairs embedded...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.05.093

    authors: Jia Y,Bi L,Li F,Chen Y,Zhang C,Zhang X

    更新日期:2008-08-08 00:00:00

  • Purification and analysis of the structure of alpha-galactosidase from Escherichia coli.

    abstract::Alpha-Galactosidase, the product of the melA gene, was purified from a strain of Escherichia coli harboring a plasmid carrying melA, which over-produced the alpha-galactosidase. An apparent molecular weight was determined to be 50 kDa. The amino acid composition of this enzyme was determined. The result indicates that...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(88)90584-0

    authors: Nagao Y,Nakada T,Imoto M,Shimamoto T,Sakai S,Tsuda M,Tsuchiya T

    更新日期:1988-02-29 00:00:00

  • Inhibition by adiponectin of IL-8 production by human macrophages upon coculturing with late apoptotic cells.

    abstract::Adiponectin, an adipocyte-derived hormone, reportedly suppresses the production of TNF-alpha and IL-6 by LPS-stimulated human or porcine macrophages, and the phagocytosis of microbeads by human macrophages. In this study, we used a high molecular weight form of adiponectin purified from human plasma to examine its eff...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.07.016

    authors: Saijo S,Nagata K,Nakano Y,Tobe T,Kobayashi Y

    更新日期:2005-09-09 00:00:00

  • ATR microspectroscopy with multivariate analysis segregates grades of exfoliative cervical cytology.

    abstract::Although cervical cancer screening in the UK has led to reductions in the incidence of invasive disease, this programme remains flawed. We set out to examine the potential of infrared (IR) microspectroscopy to allow the profiling of cellular biochemical constituents associated with disease progression. Attenuated tota...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.11.005

    authors: Walsh MJ,Singh MN,Pollock HM,Cooper LJ,German MJ,Stringfellow HF,Fullwood NJ,Paraskevaidis E,Martin-Hirsch PL,Martin FL

    更新日期:2007-01-05 00:00:00

  • Adrenomedullin stimulates cyclic AMP formation in rat vascular smooth muscle cells.

    abstract::A newly identified human peptide, adrenomedullin (AM) increased cAMP levels with dose and time dependencies in a manner similar to that of human calcitonin gene-related peptide (CGRP) in rat vascular smooth muscle cells (VSMC). The EC50 value of human AM is 2 x 10(-8) M which is slightly higher than that of CGRP (8.5 ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.1496

    authors: Ishizaka Y,Ishizaka Y,Tanaka M,Kitamura K,Kangawa K,Minamino N,Matsuo H,Eto T

    更新日期:1994-04-15 00:00:00

  • Hypoxia-induced transcriptional activation of vascular endothelial growth factor is inhibited by serum.

    abstract::Expression of vascular endothelial growth factor (VEGF) by cultured vascular smooth muscle cells was analyzed. Serum and hypoxia had nearly additive actions on VEGF mRNA expression. The function of the VEGF promoter in smooth muscle cells was analyzed using transient luciferase reporter assays. Serum and hypoxia stimu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.1947

    authors: D'Angelo G,Ladoux A,Frelin C

    更新日期:2000-01-07 00:00:00

  • Pilot study to determine the feasibility of producing protease-resistant prion protein fragments by random PCR mutagenesis.

    abstract::We report the results of a pilot study to determine the feasibility of using PCR random mutagenesis and in vitro transcription/translation to produce protease resistant full-length or truncated ovine prion proteins (PrP). Using this approach, we show the novel production of protease resistant recombinant ovine prion p...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.4450

    authors: Dear DV,Fitzmaurice TJ,Garton S,Richards SJ

    更新日期:2001-03-09 00:00:00

  • Repression of LXRα by a novel member of additional sex comb-like family, ASXL3.

    abstract::Among the members of the additional sex comb-like (ASXL) family, ASXL3 remains unexplored. Here, we showed that ASXL3 interacts with HP1α and LSD1, leading to transcriptional repression. We determined that ASXL3 depletion augments the ligand-induced transcriptional activities of LXRα and TRβ, which were repressed by A...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.10.074

    authors: Shin N,Lee YK,Park UH,Jeong JC,Um SJ

    更新日期:2014-11-28 00:00:00

  • Deuterium distribution in lactate as tracer of metabolic pathways.

    abstract::2H-NMR spectroscopy has been used to study the distribution of 2H atoms in brain lactate, after intraperitoneal injection of 2H2O. Information on brain metabolism was obtained from the ratio [(3-2H) lactate]/[(2-2H)-lactate] which was found very sensitive to the PO2 in the breathing air. The dependence of this ratio o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.7208

    authors: Vianello F,Scarpa M,Viglino P,Rigo A

    更新日期:1997-08-28 00:00:00

  • Age-dependent and cell-population-restricted LRRK2 expression in normal mouse spleen.

    abstract::Leucine-rich repeat kinase 2 (LRRK2) is the causal molecule of familial Parkinson's disease (PD), but its true physiological function remains unknown. In the normal mouse, LRRK2 is expressed in kidney, spleen, and lung at much higher levels than in brain, suggesting that LRRK2 may play an important role in these organ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.01.041

    authors: Maekawa T,Kubo M,Yokoyama I,Ohta E,Obata F

    更新日期:2010-02-12 00:00:00

  • Articular cartilage vesicles contain RNA.

    abstract::Small membrane-bound extracellular organelles known as articular cartilage matrix vesicles (ACVs) participate in pathologic mineralization in osteoarthritic articular cartilage. ACVs are also present in normal cartilage, although they have no known functions other than mineralization. Recently, RNA was identified in e...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.08.038

    authors: Mitton E,Gohr CM,McNally MT,Rosenthal AK

    更新日期:2009-10-23 00:00:00

  • Antidiabetic effect of gomisin N via activation of AMP-activated protein kinase.

    abstract::Gomisin N (GN) is a lignan derived from Schisandra chinensis. AMP-activated kinase (AMPK) has gained attention as a therapeutic target for the treatment of metabolic syndrome. Previously, we reported that GN activated the AMPK pathway and ameliorated high-fat diet (HFD)-induced hepatic steatosis. In this study, we inv...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.10.120

    authors: Jung DY,Kim JH,Lee H,Jung MH

    更新日期:2017-12-16 00:00:00

  • NELIN, a new F-actin associated protein, stimulates HeLa cell migration and adhesion.

    abstract::A new gene (GenBank Accession No. AF114264) was cloned from umbilical vein wall tissue by using RT-PCR. The gene shares high similarity to the gene encoding F-actin binding protein nexilin, so named as NELIN. A clone of 2737bp contains open reading frame of 1344bp extending from 412 to 1755. NELIN was expressed primar...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.03.082

    authors: Wang W,Zhang W,Han Y,Chen J,Wang Y,Zhang Z,Hui R

    更新日期:2005-05-20 00:00:00

  • Endothelin evoked Ca2+ transients and oscillations in A10 vascular smooth muscle cells.

    abstract::Endothelin (200 nM) evoked a rapid rise in [Ca2+]i which was then followed by a maintained elevation of [Ca2+]i. The initial transient can be explained by the release of stored Ca2+ whilst the maintained plateau is likely to be an influx of Ca2+ as it was partially inhibited by nifedipine (5 microM) and the remaining ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)91108-x

    authors: Simpson AW,Ashley CC

    更新日期:1989-09-29 00:00:00

  • A recombinant adenoviral vector encoding functional vasoactive intestinal peptide.

    abstract::Vasoactive intestinal peptide (VIP) is a small neuropeptide, which exerts pleiotropic functions. Based on its immunomodulatory, secretory, and possibly trophic effects, VIP is a valuable candidate molecule for the management of autoimmune disease. The purpose of this study was to develop a recombinant viral vector cap...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.04.171

    authors: Lodde BM,Delporte C,Goldsmith CM,Tak PP,Baum BJ

    更新日期:2004-06-18 00:00:00

  • Implication of novel biochemical property of beta-amyloid.

    abstract::Alzheimer disease (AD) is a heterogeneous disorder with a variety of molecular pathologies converging predominantly on abnormal amyloid deposition particularly in the brain. beta-Amyloid aggregation into senile plaques is one of the pathological hallmarks of AD. beta-Amyloid is generated by a proteolytic cleavage of a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.2024

    authors: Elbaum D,Brzyska M,Bacia A,Alkon DL

    更新日期:2000-01-27 00:00:00

  • Dual assay for MCLV3 activity reveals structure-activity relationship of CLE peptides.

    abstract::The dodecapeptide MCLV3 is a functional peptide, derived from the CLV3 precursor protein, which is a candidate ligand of the CLV1/CLV2 receptor complex that restricts the stem cell population in the shoot apical meristem (SAM). MCLV3 can induce shoot and root meristem consumption, the typical phenotype of transgenic p...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.09.139

    authors: Kondo T,Nakamura T,Yokomine K,Sakagami Y

    更新日期:2008-12-05 00:00:00

  • Age-related changes of Alzheimer's disease-associated proteins in cynomolgus monkey brains.

    abstract::We characterized senile plaques (SPs) immunohistochemically in cynomolgus monkey brains and also examined age-related biochemical changes of Alzheimer's disease (AD)-associated proteins in these brains from monkeys of various ages. In the neocortex of aged monkeys (>20 years old), we found SPs but no neurofibrillary t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2003.09.012

    authors: Kimura N,Tanemura K,Nakamura S,Takashima A,Ono F,Sakakibara I,Ishii Y,Kyuwa S,Yoshikawa Y

    更新日期:2003-10-17 00:00:00

  • Tissue specific distribution, clearance and vascular effects of endothelin in the pig.

    abstract::Endothelin-like immunoreactivity (-LI) was detected in the kidney, spleen, skeletal muscle, lung and in plasma of the pig. The highest tissue levels were found in the lung (1.5 +/- 0.07 pmol/g) and the lowest in the muscle (0.08 +/- 0.02 pmol/g). I.v. infusion of endothelin (20 pmol/kg/min for 20 min) increased plasma...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)92648-x

    authors: Pernow J,Hemsén A,Lundberg JM

    更新日期:1989-06-15 00:00:00

  • A new anti-tumor strategy based on in vivo tumstatin overexpression after plasmid electrotransfer in muscle.

    abstract::The NC1 domains from the different α(IV) collagen chains were found to exert anti-tumorigenic and/or anti-angiogenic activities. A limitation to the therapeutic use of these matrikines is the large amount of purified recombinant proteins, in the milligram range in mice that should be administered daily throughout the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.02.074

    authors: Thevenard J,Ramont L,Mir LM,Dupont-Deshorgue A,Maquart FX,Monboisse JC,Brassart-Pasco S

    更新日期:2013-03-22 00:00:00

  • Effect of ATP binding cassette/multidrug resistance proteins on ATP efflux of Saccharomyces cerevisiae.

    abstract::Multidrug resistance (MDR) in mammalian tumors or tissues is often associated with the overexpression of the putative drug efflux pump P-glycoprotein (Pgp). One theory concerning the mechanism of Pgp activity is that efflux of ATP is coupled to drug efflux. Evidence in support of this theory has been observed in mamma...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.5913

    authors: Boyum R,Guidotti G

    更新日期:1997-01-03 00:00:00

  • Molecular cloning and characterization of human FGF-20 on chromosome 8p21.3-p22.

    abstract::The fibroblast growth factors (FGFs) play important roles in morphogenesis, angiogenesis, tissue remodeling, and carcinogenesis. Human FGF-20 has been cloned and characterized in this study. FGF-20 encodes a 211-amino-acid polypeptide with the FGF-core domain. A strong hydrophobic region was found in the FGF-core doma...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2000.3142

    authors: Kirikoshi H,Sagara N,Saitoh T,Tanaka K,Sekihara H,Shiokawa K,Katoh M

    更新日期:2000-08-02 00:00:00

  • Secretion of insulin-like growth factor II into milk.

    abstract::125I-labeled insulin-like growth factor II (IGF-II) was infused directly into the pudic artery supplying one gland of lactating goats (n = 4). Maximum specific activity for [125I]IGF-II transferred into milk from the infused gland was reached 60 min after that in plasma and was 2.5 fold greater than in milk from the n...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(05)80322-5

    authors: Prosser CG,Fleet IR

    更新日期:1992-03-31 00:00:00

  • Nitration and chlorination of folic acid by peroxynitrite and hypochlorous acid, and the selective binding of 10-nitro-folate to folate receptor beta.

    abstract::The aim of this work was to characterize folates modified by ONOO(-) and HOCl and to evaluate the binding capacity of folates modified by ONOO(-) to folate receptor alpha and beta. For the modification of folate by ONOO(-), folic acid was reacted with the combination of PMA activated PMN and PAPA NONOate or chemically...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(02)02359-8

    authors: Nakamura M,Nagayoshi R,Ijiri K,Nakashima-Matsushita N,Takeuchi T,Matsuyama T

    更新日期:2002-10-11 00:00:00

  • Evidence for the presence of an endogenous cytosolic protein inhibitor of intestinal fucosyltransferase activities.

    abstract::Soluble endogenous inhibitory activities for glycoprotein: alpha (1-2) and alpha (1-3) fucosyltransferases are demonstrated in rat small intestinal cytosol. These inhibitors are retained on DEAE-cellulose and are eluted as two fractions A and B. Fraction B is non dialyzable, heat stable and pronase-resistant and consi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)90913-8

    authors: Martin A,Ruggiero-Lopez D,Biol MC,Louisot P

    更新日期:1990-01-30 00:00:00

  • Hearing loss through apoptosis of the spiral ganglion neurons in apolipoprotein E knockout mice fed with a western diet.

    abstract::Age-related hearing loss (ARHL) is a neurodegenerative disease associated with an aged population. ARHL is influenced by biological factors such as aging, sex difference, and atherosclerosis. The mechanisms of ARHL caused by atherosclerosis have not been previously determined in apolipoprotein E knockout (ApoE KO) mal...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.12.100

    authors: Kim YY,Chao JR,Kim C,Kim B,Thi-Thanh Nguyen P,Jung H,Chang J,Lee JH,Suh JG

    更新日期:2020-03-12 00:00:00

  • Defective human erythrocyte uroporphyrinogen decarboxylase in familial porphyria cutanea tarda: the metabolic lesion or the result of endogenous porphyrinemia?

    abstract::We have demonstrated that oral charcoal therapy is as effective as therapeutic phlebotomy in reducing porphyrinemia in porphyria cutanea tarda. The effects of immediate and sustained reduction of porphyrinemia on the catalytic properties of partially purified (approximately 200-fold) preparations of red cell uroporphy...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(88)90646-8

    authors: Mukerji SK,Pimstone NR

    更新日期:1988-07-15 00:00:00

  • Identification and characterization of estrogen and progesterone receptors from the oviduct of the American alligator (Alligator mississippiensis).

    abstract::A protein extract prepared from the egg shell fiber-secreting region of the oviduct of Alligator mississippiensis was assayed for the presence of receptors for estrogen and progesterone. In the presence of [3H]-estradiol-17 beta, the extract contained an estrogen-binding activity which reached equilibrium at 25 degree...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.6274

    authors: Vonier PM,Guillette LJ Jr,McLachlan JA,Arnold SF

    更新日期:1997-03-17 00:00:00