Functional analysis of isoflavones using patient-derived human colonic organoids.

abstract：:Using PAGE--Autoradioblotting technique we have characterized an E--FABP in human epidermal cells that is distinct from liver-, heart-, intestine- and adipose tissue-FABPs. FABP radiobinding analysis was performed directly on protein extracts without prior partial purification. E-FABP has a Mr of approximately 15 kDa ...

journal_title：Biochemical and biophysical research communications

authors： Siegenthaler G,Hotz R,Chatellard-Gruaz D,Jaconi S,Saurat JH

更新日期：1993-01-29 00:00:00

abstract：:Rodent brown adipocytes express peroxisome proliferator activated receptor-alpha (PPARalpha) and PPARgamma and while the rodent uncoupling protein-1 (UCP-1) gene contains a putative peroxisome proliferator response element (PPRE), only PPARgamma activation by thiazolidinediones increase UCP-1 mRNA levels. We have inve...

journal_title：Biochemical and biophysical research communications

authors： Teruel T,Clapham JC,Smith SA

更新日期：1999-10-22 00:00:00

abstract：:Tau, a microtubule-associated phosphoprotein, was identified as a definite component of paired helical filaments which progressively accumulate in Alzheimer's disease brain. To learn more about tau in the aged brain, we have isolated and sequenced a cDNA clone encoding tau from a cDNA library of an aged human brain. T...

journal_title：Biochemical and biophysical research communications

authors： Mori H,Hamada Y,Kawaguchi M,Honda T,Kondo J,Ihara Y

更新日期：1989-03-31 00:00:00

abstract：:Individual yeast colonies produce pulses of volatile ammonia separated by phases of medium acidification. Colonies of Saccharomyces cerevisiae mutant defective in the general amino acid permease, Gap1p, exhibit decreased ammonia production. Mutations in the S. cerevisiae amino acid sensor SPS completely abolish the co...

journal_title：Biochemical and biophysical research communications

authors： Zikánová B,Kuthan M,Ricicová M,Forstová J,Palková Z

更新日期：2002-06-28 00:00:00

abstract：:We have demonstrated previously that patients with rheumatoid arthritis (RA) show an increase in serum and synovial fluid levels of complexes between alpha1-proteinase inhibitor (alpha1PI) and IgA. These are believed to form through disulfide binding between the Cys232 residue on alpha1PI and the penultimate cysteine ...

journal_title：Biochemical and biophysical research communications

authors： Scott LJ,Russell GI,Nixon NB,Dawes PT,Mattey DL

更新日期：1999-02-24 00:00:00

abstract：:Small membrane-bound extracellular organelles known as articular cartilage matrix vesicles (ACVs) participate in pathologic mineralization in osteoarthritic articular cartilage. ACVs are also present in normal cartilage, although they have no known functions other than mineralization. Recently, RNA was identified in e...

journal_title：Biochemical and biophysical research communications

authors： Mitton E,Gohr CM,McNally MT,Rosenthal AK

更新日期：2009-10-23 00:00:00

abstract：:Because the expression of EBNA1 is prevalent in all EBV-associated tumors, it has become one of the most attractive drug targets for the discovery of anti-EBV compounds. In a cell-based reporter system, EBNA1 consistently upregulated the transcription of an oriP-Luc mini-EBV episome by 6- to 8-fold. The treatment of c...

journal_title：Biochemical and biophysical research communications

authors： Chen YL,Tsai HL,Peng CW

更新日期：2012-01-20 00:00:00

abstract：:Atrial- and brain-type natriuretic peptides (ANP and BNP, respectively) have been shown to exert potent lipolytic action in adipocytes. A family of natriuretic peptide receptors (NPRs), NPR-1, NPR-2, and NPR-3, mediates their physiologic effects. NPR-1 and NPR-2 are receptor guanylyl cyclases, while NPR-3 lacks enzyma...

journal_title：Biochemical and biophysical research communications

authors： Nakatsuji H,Maeda N,Hibuse T,Hiuge A,Hirata A,Kuroda Y,Kishida K,Kihara S,Funahashi T,Shimomura I

更新日期：2010-01-29 00:00:00

abstract：:Episomal vectors can allow efficient genetic modification of cells and have the potential advantage of avoiding chromosomal position of integration effects. Here we explore the use of an Epstein-Barr virus-based episomal vector with human embryonic stem (ES) cells, and find high initial transfection rates, but a rapid...

journal_title：Biochemical and biophysical research communications

authors： Kameda T,Smuga-Otto K,Thomson JA

更新日期：2006-11-03 00:00:00

abstract：:The recent release of COVID-19 spike glycoprotein allows detailed analysis of the structural features that are required for stabilizing the infective form of its quaternary assembly. Trying to disassemble the trimeric structure of COVID-19 spike glycoprotein, we analyzed single protomer surfaces searching for concave ...

journal_title：Biochemical and biophysical research communications

authors： Bongini P,Trezza A,Bianchini M,Spiga O,Niccolai N

更新日期：2020-07-12 00:00:00

abstract：:Extracellular acidic pH-activated chloride channel I(Cl, acid), has been characterized in HEK 293 cells and mammalian cardiac myocytes. This study was designed to characterize I(Cl,acid) in human umbilical vein endothelial cells(HUVECs). The activation and deactivation of the current rapidly and repeatedly follows the...

journal_title：Biochemical and biophysical research communications

authors： Ma ZY,Zhang W,Chen L,Wang R,Kan XH,Sun GZ,Liu CX,Li L,Zhang Y

更新日期：2008-07-04 00:00:00

abstract：:Adipose tissue-derived stem cells (ADSCs), which are easily harvested and show excellent pluripotency potential, have generated considerable interest in regenerative medicine. In this study, the differentiation of ADSCs was assessed after treatment with dental follicle cell conditioned medium (DFCCM) containing dentin...

journal_title：Biochemical and biophysical research communications

authors： Wen X,Nie X,Zhang L,Liu L,Deng M

更新日期：2011-06-10 00:00:00

abstract：BACKGROUND AND OBJECTIVE:Senescence marker protein 30 (SMP30) is assumed to behave as an anti-aging factor. Recently, we have demonstrated that deficiency of SMP30 exacerbates angiotensin II-induced cardiac hypertrophy, dysfunction and remodeling, suggesting that SMP30 may have a protective role in the heart. Thus, thi...

journal_title：Biochemical and biophysical research communications

authors： Misaka T,Suzuki S,Miyata M,Kobayashi A,Ishigami A,Shishido T,Saitoh S,Kubota I,Takeishi Y

更新日期：2013-09-13 00:00:00

abstract：:Two novel neuropeptides, urechistachykinin I (H-Leu-Arg-Gln-Ser-Gln-Phe-Val-Gly-Ser-Arg-NH2) and urechistachykinin II (H-Ala-Ala-Gly-Met-Gly-Phe-Phe-Gly-Ala-Arg-NH2), were isolated from the ventral nerve cords of the echiuroid worm, Urechis unicinctus. These peptides showed a contractile action on the inner circular b...

journal_title：Biochemical and biophysical research communications

authors： Ikeda T,Minakata H,Nomoto K,Kubota I,Muneoka Y

更新日期：1993-04-15 00:00:00

abstract：:Three unique inhibitors (SPAI-1, -+2, and -3) were first purified from porcine duodenal extract based on the Na+, K+-ATPase inhibitory activity. These peptide inhibitors had four disulfide bridges in common. The sequencing results of their S-carboxymethyl derivatives, lysilendopeptidase fragments, and chymotryptic pep...

journal_title：Biochemical and biophysical research communications

authors： Araki K,Kuroki J,Ito O,Kuwada M,Tachibana S

更新日期：1989-10-16 00:00:00

abstract：:The presence of a significantly increased content of free-cystine in skin fibroblasts from both homozygotes and heterozygotes for cystinosis emphasizes the central role of cystine in this disease, even though the primary defect responsible for cystine accumulation is yet to be determined. The studies described in this...

journal_title：Biochemical and biophysical research communications

authors： Schneider JA,Rosenbloom FM,Bradley KH,Seegmiller JE

更新日期：1967-11-30 00:00:00

abstract：:Treatment of zymogen of pancreatic-type group I phospholipase A2 (PLA2-I) by plasmin, a fibrinolytic enzyme, increases PLA2 activity as well as receptor binding activity in a dose- and time-dependent manner. Separation of plasmin-treated pro-PLA2-I by HPLC and amino acid sequence analysis of the products revealed that...

journal_title：Biochemical and biophysical research communications

authors： Nakano T,Fujita H,Kikuchi N,Arita H

更新日期：1994-01-14 00:00:00

abstract：:Intestinal challenge with cholera toxin induces the synthesis of a hormone-like protein which counteracts intestinal hypersecretion. This study shows that the protein also inhibits GABA transport across the plasma membrane of Deiters' cells in rabbits. The inhibitory action of the protein was dose dependent, and 10(3)...

journal_title：Biochemical and biophysical research communications

authors： Lange S,Lönnroth I,Palm A,Hydén H

更新日期：1985-08-15 00:00:00

abstract：:Fad104 (factor for adipocyte differentiation 104) is a novel gene expressed temporarily in the early stages of adipocyte differentiation. Previously, we showed that fad104 promotes adipocyte differentiation in mouse 3T3-L1 cells and mouse embryonic fibroblasts (MEFs). Furthermore, we reported that implanted wild-type ...

journal_title：Biochemical and biophysical research communications

authors： Kishimoto K,Kato A,Osada S,Nishizuka M,Imagawa M

更新日期：2010-06-25 00:00:00

abstract：:Expression of foreign genes in vivo is a standard method to disclose functions of specific genes and to alter physiological conditions in distinct cell types and tissues. Virus-mediated gene transfer has proved to be a valuable tool for directed gene expression in vivo complementary to transgenic approaches. However, ...

journal_title：Biochemical and biophysical research communications

authors： Ebelt H,Braun T

更新日期：2003-10-31 00:00:00

abstract：:Since we recently reported that high density lipoprotein, which contains the bioactive lipid sphingosine-1-phosphate (S1P) [Arterioscler. Thromb. Vasc. Biol. 23 (2003) 802], induced human coronary artery endothelial cell (HCEC) tube formation mediated by a Ras/Raf/ERK (extracellular signal-activated kinase) pathway, w...

journal_title：Biochemical and biophysical research communications

authors： Miura S,Tanigawa H,Matsuo Y,Fujino M,Kawamura A,Saku K

更新日期：2003-07-11 00:00:00

abstract：:We found that both RNA and cDNA preparations derived from melanocytes contain a RT-PCR inhibitor that copurified with nucleic acids. Investigation of the candidate inhibitor melanin revealed that it potently blocks PCR at concentrations below 200 ng/ml, whereas 100 microg/ml melanin was required to inhibit reverse tra...

journal_title：Biochemical and biophysical research communications

authors： Eckhart L,Bach J,Ban J,Tschachler E

更新日期：2000-05-19 00:00:00

abstract：:Serum leptin levels are elevated in subjects with exogenous obesity, indicating that obesity is associated with leptin resistance. Since in man no abnormalities have yet been found in either the genes for leptin or its receptor, the mechanism of leptin resistance in obesity remains unknown. To determine if resistance ...

journal_title：Biochemical and biophysical research communications

authors： Diamond FB Jr,Eichler DC,Duckett G,Jorgensen EV,Shulman D,Root AW

更新日期：1997-04-28 00:00:00

abstract：:DNp73 is a transactivation domain (TAD)-truncated form of p73. The ability of DNp73alpha to regulate gene expression was examined using reporter assays with luciferase gene constructs. Among various promoter-regulated reporter genes tested, heat shock factor (HSF)-responsive gene expression was selectively activated b...

journal_title：Biochemical and biophysical research communications

authors： Tanaka Y,Kameoka M,Itaya A,Ota K,Yoshihara K

更新日期：2004-05-07 00:00:00

abstract：:p120-Catenin is known to play important roles in cell-cell adhesion stability by binding to cadherin and morphological changes of cells by regulating small RhoGTPase activities. Although the expression and binding states of p120-catenin are thought to dynamically change due to morphological adaptation of endothelial c...

journal_title：Biochemical and biophysical research communications

authors： Sakamoto N,Segawa K,Kanzaki M,Ohashi T,Sato M

更新日期：2010-07-30 00:00:00

abstract：:A 64-year-old Japanese male suffering from very slowly progressive amyloidosis was studied by immunohistopathologic, mass spectrometric, and molecular genetic methods. After confirming the immunoreactivity of transthyretin (TTR) in the amyloid deposits using an anti-TTR polyclonal antibody, matrix-assisted laser desor...

journal_title：Biochemical and biophysical research communications

authors： Terazaki H,Ando Y,Misumi S,Nakamura M,Ando E,Matsunaga N,Shoji S,Okuyama M,Ideta H,Nakagawa K,Ishizaki T,Ando M,Saraiva MJ

更新日期：1999-10-22 00:00:00

abstract：:Atriopeptin (AP) is synthesized and stored in the mammalian atria as a 126 amino acid prohormone (AP126). Upon secretion, the prohormone undergoes site specific proteolysis within the atria to yield the carboxyl terminal 28 amino acid hormone (AP28). The atrial cell responsible for AP126 bioactivation has not yet been...

journal_title：Biochemical and biophysical research communications

authors： Greenwald JE,Needleman P,Siegel N,Tetens E,Biel B,Ritter D

更新日期：1992-10-30 00:00:00

abstract：:Autophagy is evolutionarily conservative in eukaryotic cells that engulf cellular long-lived proteins and organelles, and it degrades the contents through fusion with lysosomes, via which the cell acquires recycled building blocks for the synthesis of new molecules. In this study, we revealed that peiminine induces ce...

journal_title：Biochemical and biophysical research communications

authors： Lyu Q,Tou F,Su H,Wu X,Chen X,Zheng Z

更新日期：2015-06-19 00:00:00

abstract：:The homogeneous recombinant mammalian protein tyrosine phosphatase 1B (PTP1B) and Yersinia protein tyrosine phosphatase (PTPase) are inactivated by a series of low-molecular-weight S-nitrosothiols. These compounds exhibited different inhibitory activities in a time- and concentration-dependent manner with second-order...

journal_title：Biochemical and biophysical research communications

authors： Xian M,Wang K,Chen X,Hou Y,McGill A,Zhou B,Zhang ZY,Cheng JP,Wang PG

更新日期：2000-02-16 00:00:00

abstract：:Homocysteine thiolactone is a highly reactive homocysteine derivative that can react easily with proteins. Protein homocysteinylation has been suggested as a possible mechanism underlying the pathological consequences of impaired homocysteine metabolism. Homocysteine inhibits key steps of angiogenesis and tumor invasi...

journal_title：Biochemical and biophysical research communications

authors： Martínez-Poveda B,Chavarría T,Sánchez-Jiménez F,Quesada AR,Medina MA

更新日期：2003-11-21 00:00:00