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Optimized, highly efficient transfer of foreign genes into newborn mouse hearts in vivo.

Abstract：

:Expression of foreign genes in vivo is a standard method to disclose functions of specific genes and to alter physiological conditions in distinct cell types and tissues. Virus-mediated gene transfer has proved to be a valuable tool for directed gene expression in vivo complementary to transgenic approaches. However, several problems associated with routes of application, endurance of gene expression, and efficiency of infections still have to be solved. We have optimized a gene transfer protocol into hearts of newborn mice to achieve widespread long-lasting expression using adenoviral vectors. Intrathoracic injection of high-titer adenoviral preparations (10(8)pfu) led to expression of foreign genes in >71+/-8% of all heart cells for >50 days after infection without any morphological signs of cardiac malfunction, inflammation, or immune response. This approach might be adapted to long-term cellular studies in vivo since 5 months after infection up to 20% of all cardiac cells still expressed virally encoded genes. Successful and efficient expression of other gene of interest can be easily controlled by co-injection of low titers of a reporter vector encoding EGFP (10(6)pfu).

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ebelt H,Braun T

doi

10.1016/j.bbrc.2003.09.131

subject

Has Abstract

pub_date

2003-10-31 00:00:00

pages

1111-6

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006291X03019387

journal_volume

310

pub_type

杂志文章

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