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Unusual RNA polymerase content of Trypanosoma brucei nuclei.

Abstract：

:Nuclei were isolated from bloodstream forms of Trypanosoma brucei by nitrogen cavitation and sedimentation through percoll density gradients. Transcription studies with these nuclei in vitro demonstrated features not seen with other eukaryotes: RNA synthesis was much greater in the presence of Mn2+ than with Mg2+ and was sensitive to high concentrations (10-100 micrograms/ml) of alpha-amanitin at all salt concentrations tested (25-300 mM ammonium sulphate). RNA polymerase extracted from nuclei by sonication at high ionic strength chromatographed as a single peak, sensitive to high alpha-amanitin concentrations, on DEAE-sephadex under conditions which resolved the classic three RNA polymerase forms when rat liver nuclear extracts were used.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Earnshaw DL,Beebee TJ,Gutteridge WE

doi

10.1016/0006-291x(85)91316-6

subject

Has Abstract

pub_date

1985-09-16 00:00:00

pages

844-8

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(85)91316-6

journal_volume

131

pub_type

杂志文章

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