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Escherichia coli O157:H7 LPS O-side chains and pO157 are required for killing Caenorhabditis elegans.

Abstract：

:As a model host, the nematode Caenorhabditis elegans has been used for studying unknown pathogen-host interactions and identifying novel virulence factors in bacterial pathogens. Among the bacterial pathogens that can induce death of C. elegans is enterohemorrhagic Escherichia coli (EHEC) O157:H7, a major serotype of EHEC that causes hemorrhagic colitis and hemolytic uremic syndrome in humans and animals. However, it is unknown which EHEC O157:H7 factors are required for nematode death. In this study, bacterial ability to kill C. elegans was tested for several EHEC O157:H7 wild-type and mutant strains missing one virulence-associated factor, including Shiga toxins, enterohemolysin, pO157 (a large virulence plasmid in EHEC O157:H7), Type 3 secretion system, LuxS, and lipopolysaccharide (LPS) O-side chains. Our results demonstrate that only mutants lacking either pO157 or LPS O-side chains cause full attenuation in killing C. elegans. The LPS O-side chain-defective ΔperA mutant strain was not able to colonize in the intestine even at 24h post-feeding with C. elegans, while the wild-type strain began to accumulate and colonize in the intestine as early as 3h post-feeding. A simple complementation of the mutant strain with the plasmid carrying the intact perA gene in trans completely restored the production of LPS O-side chains, as well as the ability to kill C. elegans. Our results show that pO157 and PerA are required for EHEC O157:H7 to kill C. elegans.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Youn M,Lee KM,Kim SH,Lim J,Yoon JW,Park S

doi

10.1016/j.bbrc.2013.05.111

subject

Has Abstract

pub_date

2013-07-05 00:00:00

pages

388-93

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(13)00918-2

journal_volume

436

pub_type

杂志文章

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