Abstract:
:The onset of labour is associated with an increase in prostaglandin synthesis in amnion which appears to be mediated at least in part by an increase in cyclo-oxygenase (COX) expression. We have tested the hypothesis that COX expression is controlled in amnion by the binding of a protein to the COX mRNA which may inhibits its translation, as has been seen in vascular smooth muscle cells. Using differential RNA extraction protocols which extract either total mRNA or only mRNA which is not protein bound, we have found that, in amnion, the increase in COX-2 expression seen after the onset of labour is entirely in the protein bound fraction. Unlike in vascular smooth muscle, increased expression of COX-2 in amnion cells is therefore associated with increased rather than decreased protein binding to the mRNA. This protein may be involved in translation initiation or elongation or in mRNA stability. There does not appear to be a protein bound COX-1 mRNA fraction.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Slater D,Berger L,Moore G,Bennett Pdoi
10.1006/bbrc.1994.2149subject
Has Abstractpub_date
1994-08-30 00:00:00pages
67-71issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(84)72149-8journal_volume
203pub_type
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.2000.4142
更新日期:2001-01-19 00:00:00