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The Vpr protein of human immunodeficiency virus type 1 binds to nucleocapsid protein p7 in vitro.

Abstract：

:The Vpr protein of human immunodeficiency virus type 1 (HIV-1) is incorporated into the virion by the Gag polyprotein precursor Pr55gag. The importance of the p6gag sequence at the C-terminal end of Pr55gag has a crucial role in Vpr incorporation. To identify the Gag sequences directly involved in Vpr binding, we compared the Vpr binding affinities of the 71 amino acid nucleocapsid protein p7, the C-terminal peptide (35-71) p7C and p6gag by affinity chromatography. p7 and p7C have the strongest Vpr binding activities compared to p6gag. These results suggest that the nucleocapsid protein and its C-terminal domain may be important for the incorporation of Vpr into the mature HIV-1 virion and the subsequent localisation of viral nucleic acid to the cell nucleus by Vpr.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Li MS,Garcia-Asua G,Bhattacharyya U,Mascagni P,Austen BM,Roberts MM

doi

10.1006/bbrc.1996.0061

subject

Has Abstract

pub_date

1996-01-05 00:00:00

pages

352-5

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(96)90061-3

journal_volume

218

pub_type

杂志文章

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