Acetylation and level of mitochondrial transcription factor A in several organs of young and old rats.

Abstract:

:To gain further information on the role of mitochondrial transcription factor A (TFAM) in mitochondrial biogenesis, we studied the post-translational modifications of the protein in 6- and 28-month-old rat liver. Mass spectrometry and immunoblot analysis revealed that TFAM was acetylated at a single lysine residue and that the level of acetylation did not change with age. The measurement of the content of TFAM and of mitochondrial DNA (mtDNA) in several organs (cerebellum, heart, kidney, and liver) of young and old rats showed an age-related increase of mtDNA and TFAM in all the organs analyzed, except in heart. These data are discussed in the light of the multiple roles of TFAM in mitochondrial biogenesis and of the age-related change of the mitochondrial transcription.

authors

Dinardo MM,Musicco C,Fracasso F,Milella F,Gadaleta MN,Gadaleta G,Cantatore P

doi

10.1016/s0006-291x(02)03008-5

subject

Has Abstract

pub_date

2003-01-31 00:00:00

pages

187-91

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006291X02030085

journal_volume

301

pub_type

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