Increased immunoreactive neuropeptide Y in platelets of spontaneously hypertensive rats (SHR).

abstract：:Corneal epithelial stem cells are believed to reside in the basal layer of the limbal epithelium, but no definitive cell surface markers have been identified. For keratinocytes, stem/progenitor cells are known to be enriched by cell surface markers, integrin alpha(6) and CD71, as a minor subpopulation which shows high...

journal_title：Biochemical and biophysical research communications

authors： Hayashi R,Yamato M,Saito T,Oshima T,Okano T,Tano Y,Nishida K

更新日期：2008-03-07 00:00:00

abstract：:Pancreatic ductal carcinoma (PDC) remains one of the most intractable human malignancies. To obtain insight into the molecular pathogenesis of PDC, we constructed a retroviral cDNA expression library with total RNA isolated from the PDC cell line MiaPaCa-2. Screening of this library with the use of a focus formation a...

journal_title：Biochemical and biophysical research communications

authors： Fujiwara S,Yamashita Y,Choi YL,Wada T,Kaneda R,Takada S,Maruyama Y,Ozawa K,Mano H

更新日期：2005-12-16 00:00:00

abstract：:Treatment of the rat C6 glioma cell line with interleukin-1 beta (IL-1 beta) resulted in an accumulation of cytosolic phospholipase A2 (cPLA2) in mRNA level. The increase of cPLA2 in mRNA level was observed at 6 hours after treatment of IL-1 beta and reached to the maximal level at 12 hours. The accumulation also rema...

journal_title：Biochemical and biophysical research communications

authors： Ozaki M,Morii H,Qvist R,Watanabe Y

更新日期：1994-11-30 00:00:00

abstract：:Sulfation is an important pathway in the metabolism of thyroid hormones. Sulfated iodothyronines are elevated in nonthyroidal illnesses and in the normal human fetal circulation. We assayed and characterized COS-1 cell expressed recombinant human liver dehydroepiandrosterone sulfotransferase (DHEA ST or SULT2A1) and e...

journal_title：Biochemical and biophysical research communications

authors： Li X,Anderson RJ

更新日期：1999-10-05 00:00:00

abstract：:The ouabain-resistant and ouabain-sensitive alpha-subunit cRNAs in various molar ratios were injected into Xenopus oocytes together with cRNA for the beta-subunit. The ouabain-resistant ATPase activity, as well as ouabain-resistant Rb+ uptake, of the injected oocytes increased linearly with increasing the amount of cR...

journal_title：Biochemical and biophysical research communications

authors： Takeda K,Kawamura M

更新日期：2001-02-09 00:00:00

abstract：:The vast majority of physiological processes in living cells are mediated by protein-protein interactions often specified by particular protein sequence motifs. PDZ domains, composed of 80-100 amino acid residues, are an important class of interaction motif. Among the PDZ-containing proteins, glutaminase interacting p...

journal_title：Biochemical and biophysical research communications

authors： Zencir S,Ovee M,Dobson MJ,Banerjee M,Topcu Z,Mohanty S

更新日期：2011-08-12 00:00:00

abstract：:A biological synthesis of phosphocitrate is described from precursors, citrate and adenosinetriphosphate reacting in the presence of rat liver homogenate. Identity of the newly formed product was examined by enzymatic digestion of reactions mixtures, HPLC chromatography and 1H-NMR spectra. Authenticity of product was ...

journal_title：Biochemical and biophysical research communications

authors： Moro L,Stagni N,Luxich E,Sallis JD,de Bernard B

更新日期：1990-07-16 00:00:00

abstract：:Human placental poly(ADP-ribose) polymerase was purified and the NH2-terminal amino acid sequences of 16 KDa and 40 KDa chymotryptic peptides were determined. Screening of a lambda gt11 cDNA library of normal human placenta with a 51-mer oligodeoxyribonucleotide yielded one clone with a 1.8 Kb insert and two clones wi...

journal_title：Biochemical and biophysical research communications

authors： Suzuki H,Uchida K,Shima H,Sato T,Okamoto T,Kimura T,Miwa M

更新日期：1987-07-31 00:00:00

abstract：:To develop antibodies against the plasma membrane 3,3',5-triiodo-L-thyronine (T3) binding protein (M.W. 55,000), rabbits were immunized with formalin-fixed GH3 cells or highly purified plasma membranes from these cells. Antibodies were screened by immunoprecipitation using detergent solubilized N-bromoacetyl-[125I]T3-...

journal_title：Biochemical and biophysical research communications

authors： Hasumura S,Rossi B,Alderson R,Pastan I,Cheng S

更新日期：1984-11-14 00:00:00

abstract：:Aplastic anemia (AA) is a serious blood system disease that threatens human health. At present, the main cause of this disease is believed to be immune hyperfunction. However, the specific metabolic mode involved in the occurrence of lymphocytes in AA is still unknown. In addition, whether rapamycin, a specific blocke...

journal_title：Biochemical and biophysical research communications

authors： Liu SL,Zhou YM,Tang DB,Zhou N,Zheng WW,Tang ZH,Duan CW,Chen J

更新日期：2019-10-15 00:00:00

abstract：UNLABELLED:Pervious biochemical and hemodynamic studies have highlighted the important role of εPKC in cardioprotection during ischemic preconditioning. However, little is known about the electrophysiological consequences of εPKC modulation in ischemic hearts. Membrane permeable peptide εPKC selective activator and inh...

journal_title：Biochemical and biophysical research communications

authors： Restivo M,Kozhevnikov DO,Qu YS,Yue Y,Mochly-Rosen D,El-Sherif N,Boutjdir M

更新日期：2012-09-21 00:00:00

abstract：:Evidence of a complex formation is a crucial step in the structural studies of ligand-receptor interactions. Here we presented a simple and fast approach for qualitative screening of the complex formation between the chimeric extracellular domain of the nicotinic acetylcholine receptor (α7-ECD) and three-finger protei...

journal_title：Biochemical and biophysical research communications

authors： Kulbatskii DS,Shulepko MA,Sluchanko NN,Yablokov EO,Kamyshinsky RA,Chesnokov YM,Kirpichnikov MP,Lyukmanova EN

更新日期：2020-10-29 00:00:00

abstract：:In order to study the intracellular regulatory function of glia maturation factor (GMF) in neuronal cells, we achieved a 10-fold overexpression of GMF in the rat pheochromocytoma cell line PC12 by infection with a replication-defective human adenovirus carrying a rat GMF cDNA insert. These cells showed a 3.6-fold incr...

journal_title：Biochemical and biophysical research communications

authors： Zaheer A,Lim R

更新日期：1998-09-18 00:00:00

abstract：:The protein kinase CK2 holoenzyme is composed of two regulatory beta- and two catalytic alpha- or alpha(')-subunits. There is ample evidence for the binding of individual subunits of CK2 to various cellular proteins and, moreover, for functions of the individual subunits, which are different from their roles in the ho...

journal_title：Biochemical and biophysical research communications

authors： Faust M,Kartarius S,Schwindling SL,Montenarh M

更新日期：2002-08-09 00:00:00

abstract：:We present here the biosynthesis of AgNps from the aqueous extract of H. thebaica fruit, and monitored through UV-Vis spectrophotometer. The functional group were characterized through ATR-FTIR spectroscopy, the particle size, morphologies and elemental composition of the nanoparticles were investigated by using TEM, ...

journal_title：Biochemical and biophysical research communications

authors： Bello BA,Khan SA,Khan JA,Syed FQ,Mirza MB,Shah L,Khan SB

更新日期：2017-08-26 00:00:00

abstract：:Measurements of the cytosolic calcium concentration in single cells of the clonal endocrine pancreatic glucagon-secreting cell line INR1 G9 revealed the existence of spontaneous calcium oscillations in 20-70% of these cells. Inhibition of these spontaneous oscillations by thapsigargin as well as the phospholipase C in...

journal_title：Biochemical and biophysical research communications

authors： Bode HP,Yule DI,Fehmann HC,Göke B,Williams JA

更新日期：1994-11-30 00:00:00

abstract：:Glycine-rich RNA-binding proteins play an important role in post-transcriptional regulation of gene expression, including RNA processing, and are known to be up-regulated in response to a number of external stimuli. However, their regulation in response to salinity stress has not been reported. We have isolated a ligh...

journal_title：Biochemical and biophysical research communications

authors： Aneeta,Sanan-Mishra N,Tuteja N,Kumar Sopory S

更新日期：2002-09-06 00:00:00

abstract：:We have cloned cDNAs encoding endothelial nitric oxide synthase (ecNOS) from a human fetal liver cDNA library. Overproduction of ecNOS in a baculovirus/insect cell expression system in conventional medium yielded a large amount of ecNOS protein localized in particulate components, but ecNOS activity was low. This acti...

journal_title：Biochemical and biophysical research communications

authors： Seo HG,Fujii J,Soejima H,Niikawa N,Taniguchi N

更新日期：1995-03-08 00:00:00

abstract：:The effect of tyrosine-phosphorylation on the conformation of three tyrosine-based immunoreceptor activation motifs, zeta(69-86), zeta(106-126), and zeta(138-155), located in the T cell receptor/CD3 zeta-chain was investigated. Circular dichroism and Fourier-transform infrared spectroscopy of the nonphosphorylated and...

journal_title：Biochemical and biophysical research communications

authors： Laczkó I,Hollósi M,Vass E,Hegedüs Z,Monostori E,Tóth GK

更新日期：1998-01-26 00:00:00

abstract：:The appearance of Fas receptor at the surface of pancreatic beta-cells affected by progressive insulitis strongly suggests that Fas-mediated beta-cell apoptosis plays an important role in the pathogenesis of type 1 diabetes. In support of this concept, the present study has shown that islet cells from NOD mice and the...

journal_title：Biochemical and biophysical research communications

authors： Augstein P,Dunger A,Salzsieder C,Heinke P,Kubernath R,Bahr J,Fischer U,Rettig R,Salzsieder E

更新日期：2002-01-11 00:00:00

abstract：:IL-17 plays an important role in gut homeostasis. However, the role of IL-17F in intestinal tumorigenesis has not been addressed. Here we demonstrate that ablation of IL-17F significantly inhibits spontaneous intestinal tumorigenesis in the small intestine of Apc(Min/+) mice. IL-17F ablation decreased IL-1β and Cox-2 ...

journal_title：Biochemical and biophysical research communications

authors： Chae WJ,Bothwell AL

更新日期：2011-10-14 00:00:00

abstract：:Resveratrol (3,5,4'-trihydroxystilbene) is a naturally occurring phytoalexin, found in grapes and wine, which has been reported to exert a variety of important pharmacological effects. We have investigated the activity of resveratrol on proliferation and differentiation of the promyelocitic cell line HL-60. A concentr...

journal_title：Biochemical and biophysical research communications

authors： Ragione FD,Cucciolla V,Borriello A,Pietra VD,Racioppi L,Soldati G,Manna C,Galletti P,Zappia V

更新日期：1998-09-08 00:00:00

abstract：:The crude extracts from Sphingomonas paucimobilus containing 2-hydroxy fatty acid-rich sphingolipids were found to oxidize [1-14C]-myristate to 2-hydroxymyristate in the presence of NADH. The myristate-oxidation activity was partially purified about 290-fold from the cell-free extracts by ammonium sulfate fractionatio...

journal_title：Biochemical and biophysical research communications

authors： Matsunaga I,Kusunose E,Yano I,Ichihara K

更新日期：1994-06-30 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARγ) participates in the process of insulin resistance (IR), a crucial pathophysiology in non-alcoholic fatty liver disease (NAFLD). Meanwhile, suppressor of cytokine signaling3 (SOCS3) also regulates IR in NAFLD. Both PPARγ and SOCS3 play a role in NAFLD through reg...

journal_title：Biochemical and biophysical research communications

authors： Bi J,Sun K,Wu H,Chen X,Tang H,Mao J

更新日期：2018-04-15 00:00:00

abstract：:alpha 2,6 sialyltransferase towards the N-acetyllactosaminyl sequence (alpha 2,6 ST, E.C. 2.4.99.1) is one of the major sialyltransferases in human colonic cells; it strongly increases in human colorectal tumors and is largely expressed in fetal and neonatal rat colon. In this study we demonstrate that human colon car...

journal_title：Biochemical and biophysical research communications

authors： Dall'Olio F,Malagolini N,Serafini-Cessi F

更新日期：1992-05-15 00:00:00

abstract：:Arl13b is an atypical Arf/Arl-family GTPase consisting of an extending large C-terminal region (C domain) and Arf-homologous GTP-binding motifs in the N terminus (N domain). Although Arl13b appears to be involved in cilia formation, its precise function and roles of the domains remain unknown. Here, we show the unique...

journal_title：Biochemical and biophysical research communications

authors： Hori Y,Kobayashi T,Kikko Y,Kontani K,Katada T

更新日期：2008-08-15 00:00:00

abstract：:Iron is required in all organisms for crucial functions, as a number of proteins need iron for activity. Mutations of the genes encoding proteins involved in iron uptake, transport, and utilization result in various human disorders or animal models with very different clinical presentations and organ involvement. Howe...

journal_title：Biochemical and biophysical research communications

authors： Rötig A,Chantrel-Groussard K,Munnich A,Rustin P

更新日期：2001-03-02 00:00:00

abstract：:The aminoglycoside antibiotic (AGA) neomycin is a known agonist of the extracellular calcium-sensing receptor (CaR). To test whether other AGA drugs stimulate the CaR, we studied the relative effects of four AGAs on intracellular Ca(2+) concentration ([Ca(2+)](i)) using CaR-transfected human embryonic kidney (HEK)-293...

journal_title：Biochemical and biophysical research communications

authors： McLarnon S,Holden D,Ward D,Jones M,Elliott A,Riccardi D

更新日期：2002-09-13 00:00:00

abstract：:Recent years have seen considerable evolution in our understanding of the mechanisms of oxygen activation by non-heme iron enzymes, with high-valent iron-oxo intermediates coming to the forefront as formidably potent oxidants. In the absence of substrate, the generation of vividly colored chromophores deriving from th...

journal_title：Biochemical and biophysical research communications

authors： Farquhar ER,Koehntop KD,Emerson JP,Que L Jr

更新日期：2005-12-09 00:00:00

abstract：:Replacement of leucine in the chemotactic peptide For-Met-Leu-Phe by the sterically constrained amino acids alpha-aminoisobutyric acid and aminocyclohexanecarboxylic acid affords compounds of equal or greater activity than the parent. NMR studies indicate that the parent compound is present as a beta-sheet in solution...

journal_title：Biochemical and biophysical research communications

authors： Semus SF,Becker EL,Toniolo C,Freer RJ

更新日期：1988-12-15 00:00:00