当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Enrichment of corneal epithelial stem/progenitor cells using cell surface markers, integrin alpha6 and CD71.

Enrichment of corneal epithelial stem/progenitor cells using cell surface markers, integrin alpha6 and CD71.

Abstract：

:Corneal epithelial stem cells are believed to reside in the basal layer of the limbal epithelium, but no definitive cell surface markers have been identified. For keratinocytes, stem/progenitor cells are known to be enriched by cell surface markers, integrin alpha(6) and CD71, as a minor subpopulation which shows high integrin alpha(6) and low CD71 expressions (alpha(6)(bri)/CD71(dim)). In the present study, we investigated the possibility that corneal epithelial stem cells can be enriched by integrin alpha(6) and CD71. The alpha(6)(bri)/CD71(dim) cells were separated by fluorescence-activated cell sorting, as a minor subpopulation of the limbal epithelial cells. They were enriched for relatively small cells, showing a higher clonogenic capacity and expression of stem cell markers, but a lower expression of differentiation markers, compared to other cell populations. The cells were localized immunohistochemically in the basal region of the limbal epithelium. These results indicate that the alpha(6)(bri)/CD71(dim) subpopulation enriched corneal epithelial stem cells.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Hayashi R,Yamato M,Saito T,Oshima T,Okano T,Tano Y,Nishida K

doi

10.1016/j.bbrc.2007.12.077

subject

Has Abstract

pub_date

2008-03-07 00:00:00

pages

256-63

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)02683-6

journal_volume

367

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Deficiencies in complex I subunits of the respiratory chain in Parkinson's disease.

    abstract：:Immunoblotting studies on mitochondria prepared from the striata of patients who died of Parkinson's disease were performed using specific antisera against Complexes I, III and IV. In 4 out of 5 patients with Parkinson's disease, the 30-, 25- and 24-kDa subunits of Complex I were moderately to markedly decreased. No c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91141-8

    authors： Mizuno Y,Ohta S,Tanaka M,Takamiya S,Suzuki K,Sato T,Oya H,Ozawa T,Kagawa Y

    更新日期：1989-09-29 00:00:00

  • Insulin-like growth factors in sheep thyroid cells: action, receptors and production.

    abstract：:Sheep thyroid cells cultured in serum-free medium were used to study the biologic activity, binding, and production of the insulin-like growth factors (IGFs). IGF-I, IGF-II, and insulin stimulated thyroid cell division. Abundant, specific IGF receptors on sheep thyroid cell membranes were identified by binding displac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90219-7

    authors： Bachrach LK,Eggo MC,Hintz RL,Burrow GN

    更新日期：1988-08-15 00:00:00

  • Sirt3 binds to and deacetylates mitochondrial pyruvate carrier 1 to enhance its activity.

    abstract：:Mitochondrial pyruvate carrier (MPC), composed of MPC1 and MPC2, can modulate pyruvate oxidation in mitochondrial and MPC1 expression correlates with poor prognosis of multiple cancers. Here, we reported that MPC1 is acetylated and its main acetylation sites are: K45 and K46. Sirt3 binds to and deacetylates MPC1. High...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.11.036

    authors： Liang L,Li Q,Huang L,Li D,Li X

    更新日期：2015-12-25 00:00:00

  • Lyso(bis)phosphatidic acid: a novel source of arachidonic acid for oxidative metabolism by rabbit alveolar macrophages.

    abstract：:To identify the source of arachidonic acid utilized for eicosanoid production, rabbit alveolar macrophages were challenged with 1.0 microM 12-O-tetradecanoylphorbol-13-acetate (TPA) or 3.8 microM Ca+2 ionophore A23187 for 3 h. Upon stimulation with TPA, a loss of [3H]arachidonic acid from phosphatidylcholine, phosphat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90487-5

    authors： Cochran FR,Connor JR,Roddick VL,Waite BM

    更新日期：1985-07-31 00:00:00

  • Aminoglycoside antibiotics induce pH-sensitive activation of the calcium-sensing receptor.

    abstract：:The aminoglycoside antibiotic (AGA) neomycin is a known agonist of the extracellular calcium-sensing receptor (CaR). To test whether other AGA drugs stimulate the CaR, we studied the relative effects of four AGAs on intracellular Ca(2+) concentration ([Ca(2+)](i)) using CaR-transfected human embryonic kidney (HEK)-293...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02133-2

    authors： McLarnon S,Holden D,Ward D,Jones M,Elliott A,Riccardi D

    更新日期：2002-09-13 00:00:00

  • GPR35 is a functional receptor in rat dorsal root ganglion neurons.

    abstract：:GPR35, previously an orphan G-protein coupled receptor, is a receptor for kynurenic acid. Here we examine the distribution of GPR35 in the rat dorsal root ganglion (DRG) and the effects of its selective activation. GPR35 was expressed predominantly by small- to medium-diameter neurons of the DRG. Many of these same ne...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.10.197

    authors： Ohshiro H,Tonai-Kachi H,Ichikawa K

    更新日期：2008-01-11 00:00:00

  • The RAB GTPase RAB18 modulates macroautophagy and proteostasis.

    abstract：:Macroautophagy is a conserved degradative pathway and its deterioration is linked to disturbances in cellular proteostasis and multiple diseases. Here, we show that the RAB GTPase RAB18 modulates autophagy in primary human fibroblasts. The knockdown of RAB18 results in a decreased autophagic activity, while its overex...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.03.112

    authors： Feldmann A,Bekbulat F,Huesmann H,Ulbrich S,Tatzelt J,Behl C,Kern A

    更新日期：2017-05-06 00:00:00

  • Changes of protein kinase C subspecies in staphylococcal enterotoxin-B-induced thymocyte apoptosis.

    abstract：:Our previous studies demonstrated that intravenous administration of staphylococcal enterotoxin B (SEB) to BALB/c mice resulted in thymocyte apoptosis. In the present study, we evaluated the role of protein kinase C (PKC) in thymocyte apoptosis induced by SEB. Our results showed that the level of protein phosphorylati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2244

    authors： Lin YS,Kao SF,Jan MS,Cheng ML,Wing LY,Chang WC,Lei HY,Lin MT

    更新日期：1995-08-24 00:00:00

  • 7,8-Dihydroxyflavone ameliorates high-glucose induced diabetic apoptosis in human retinal pigment epithelial cells by activating TrkB.

    abstract：BACKGROUND:In diabetic retinopathy, prolonged high-level blood glucose induced significant impairments among various retinal tissues, including retinal pigment epithelial (RPE) cells. In an in vitro model of human RPE cells, we evaluated whether 7,8-Dihydroxyflavone (DHF) may effectively prevent high glucose-induced di...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.11.007

    authors： Yu X,Liu Q,Wang X,Liu H,Wang Y

    更新日期：2018-01-01 00:00:00

  • The radial glia antibody RC2 recognizes a protein encoded by Nestin.

    abstract：:The RC2 antibody is widely used to label mouse radial glial cells in the developing central nervous system. While the antibody is known to recognize a 295-kDa intermediate filament proximal protein, the gene encoding the RC2 antigen remains to be identified. Here, we present evidences clearly demonstrating that Nestin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.03.074

    authors： Park D,Xiang AP,Zhang L,Mao FF,Walton NM,Choi SS,Lahn BT

    更新日期：2009-05-08 00:00:00

  • Kaiso is a key regulator of spleen germinal center formation by repressing Bcl6 expression in splenocytes.

    abstract：:Kaiso was previously described as a methylated DNA-binding protein and a transcription repressor interacting with the corepressor protein complex NCoR. In the current study, we show that generation-3 Kaiso knockout mice show a phenotype of splenomegaly and large diffused germinal centers (GC). In the spleens of Kaiso ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.11.046

    authors： Koh DI,Yoon JH,Kim MK,An H,Kim MY,Hur MW

    更新日期：2013-12-13 00:00:00

  • Substitution of the ISP alpha subunit of biphenyl dioxygenase from Pseudomonas results in a modification of the enzyme activity.

    abstract：:We have constructed a hybrid multicomponent dioxygenase gene cluster in which the bphA1 gene, coding for ISP alpha subunit of biphenyl dioxygenase from Pseudomonas pseudoalcaligenes KF707, has been replaced by the bedC1 gene encoding the corresponding subunit of benzene dioxygenase from P. putida ML2. Escherichia coli...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2546

    authors： Tan HM,Cheong CM

    更新日期：1994-10-28 00:00:00

  • Serine/threonine/tyrosine-interacting-like protein 1 (STYXL1), a pseudo phosphatase, promotes oncogenesis in glioma.

    abstract：:Phosphatases play an important role in cellular signaling and are often found dysregulated in cancers including glioblastoma (GBM). A comprehensive bioinformatics analysis of phosphatases (n = 403) in multiple datasets revealed their deregulation in GBM. Among the differentially regulated phosphatases (n = 186; 46.1%)...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2019.05.093

    authors： Tomar VS,Baral TK,Nagavelu K,Somasundaram K

    更新日期：2019-07-12 00:00:00

  • Upregulation of survivin by leptin/STAT3 signaling in MCF-7 cells.

    abstract：:Leptin and its receptors are overexpressed in breast cancer tissues and correlate with poor prognosis. Survivin, a member of the inhibitor of apoptosis protein (IAP) gene family, is generally upregulated in tumor tissues and prevents tumor cells from apoptosis. Here we showed that leptin upregulated survivin mRNA and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.04.004

    authors： Jiang H,Yu J,Guo H,Song H,Chen S

    更新日期：2008-03-28 00:00:00

  • Bipartite nuclear localization signal of matrin 3 is essential for vertebrate cells.

    abstract：:Matrin 3, a nuclear matrix protein has potential (1) to withhold promiscuously edited RNAs within the nucleus in cooperation with p54(nrb) and PSF, (2) to mediate NMDA-induced neuronal death, and (3) to modulate promoter activity of genes proximal to matrix/scaffold attachment region (MAR/SAR). We identified a biparti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.12.191

    authors： Hisada-Ishii S,Ebihara M,Kobayashi N,Kitagawa Y

    更新日期：2007-03-02 00:00:00

  • Glucose effect on the expression of 150 kDa oxygen-regulated protein in HeLa cells.

    abstract：:Chaperones assist in the correct folding of newly synthesised proteins in the endoplasmic reticulum (ER) of cells, this being essential for the translocation of protein molecules to specific subcellular compartments, extracellular matrix or to biological fluids. The biosynthesis of some ER chaperones is regulated by g...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.09.139

    authors： Cechowska-Pasko M,Bańkowski E,Chene P

    更新日期：2005-11-25 00:00:00

  • Effect of cimetidine on intratumoral cytokine expression in an experimental tumor.

    abstract：:To evaluate the immunomodulatory effects of histamine in vivo, we analyzed an experimental syngenic tumor model using a colon adenocarcinoma cell line, CT-26, in Balb/c mice. In this model, distinct tumor growth was observed around 6 days after inoculation. Daily administration of cimetidine (0.12 mg/kg/day) significa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4487

    authors： Takahashi K,Tanaka S,Ichikawa A

    更新日期：2001-03-01 00:00:00

  • Full sequence of neurocalcin, a novel calcium-binding protein abundant in central nervous system.

    abstract：:We determined the cDNA sequence for neurocalcin, a novel calcium-binding protein in bovine brain. This clone (pCalN) has 582 nucleotides in the open reading frame including the termination codon TGA, 11 nucleotides of the 5' leader and 1251 nucleotides of the 3' noncoding region. The deduced amino acid sequence reveal...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80968-4

    authors： Okazaki K,Watanabe M,Ando Y,Hagiwara M,Terasawa M,Hidaka H

    更新日期：1992-05-29 00:00:00

  • Real-time SPR characterization of the interactions between multi-epitope proteins and antibodies against classical swine fever virus.

    abstract：:The envelope glycoprotein E2 is the major immunodominant protein of the classical swine fever virus and can induce neutralizing antibodies and protective host-immune responses in infected swine. We designed, expressed, and purified multi-epitope protein (GST-BT22) that contains a tandem repeat of the E2 antigenic-dete...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.12.104

    authors： Tian H,Hou X,Liu X

    更新日期：2013-02-08 00:00:00

  • Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas.

    abstract：:The oyster vasa-like gene was previously demonstrated to be specifically expressed in germline cells of adult oysters Crassostrea gigas. In the present study, this gene was used as a molecular marker to establish the developmental pattern of germline cells during oyster ontogenesis, using whole-mount in situ hybridiza...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.06.009

    authors： Fabioux C,Huvet A,Lelong C,Robert R,Pouvreau S,Daniel JY,Minguant C,Le Pennec M

    更新日期：2004-07-23 00:00:00

  • Genome-wide location analysis reveals an important overlap between the targets of the yeast transcriptional regulators Rds2 and Adr1.

    abstract：:Upon glucose depletion, a massive reprogramming of gene expression occurs in the yeast Saccharomyces cerevisiae for the use of alternate carbon sources such as the nonfermentable compounds ethanol and glycerol. This process is mediated by the master kinase Snf1 that controls the activity of various targets including t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.05.151

    authors： Soontorngun N,Baramee S,Tangsombatvichit C,Thepnok P,Cheevadhanarak S,Robert F,Turcotte B

    更新日期：2012-07-13 00:00:00

  • Probing the catalytic mechanism of bovine pancreatic deoxyribonuclease I by chemical rescue.

    abstract：:Previous structural and mutational studies of bovine pancreatic deoxyribonuclease I (bpDNase I) have demonstrated that the active site His134 and His252 played critical roles in catalysis. In our present study, mutations of these two His residues to Gln, Ala or Gly reduced the DNase activity by a factor of four to fiv...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.11.078

    authors： Chen WJ,Lai PJ,Lai YS,Huang PT,Lin CC,Liao TH

    更新日期：2007-01-19 00:00:00

  • Molecular hierarchy in neurons differentiated from mouse ES cells containing a single human chromosome 21.

    abstract：:Defects in neurogenesis and neuronal differentiation in the fetal brain of Down syndrome (DS) patients lead to the apparent neuropathological abnormalities and contribute to the phenotypic characters of mental retardation, and premature development of Alzheimer's disease, those being the most common phenotype in DS. I...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.12.091

    authors： Wang CC,Kadota M,Nishigaki R,Kazuki Y,Shirayoshi Y,Rogers MS,Gojobori T,Ikeo K,Oshimura M

    更新日期：2004-02-06 00:00:00

  • Shiga-like toxin-based high-efficiency and receptor-specific intracellular delivery system for a protein.

    abstract：:The cell-specific cytosolic delivery of functional macromolecules with high efficiency is of great significance in molecular medicine and biotechnology. Herein, we present a Shiga-like toxin II-based high-efficiency and receptor-specific intracellular delivery system. We designed and constructed the Shiga-like toxin-b...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.07.122

    authors： Ryou JH,Sohn YK,Hwang DE,Kim HS

    更新日期：2015-09-04 00:00:00

  • Silencing of poly(ADP-ribose) glycohydrolase sensitizes lung cancer cells to radiation through the abrogation of DNA damage checkpoint.

    abstract：:Poly(ADP-ribose) glycohydrolase (PARG) is a major enzyme that plays a role in the degradation of poly(ADP-ribose) (PAR). PARG deficiency reportedly sensitizes cells to the effects of radiation. In lung cancer, however, it has not been fully elucidated. Here, we investigated whether PARG siRNA contributes to an increas...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.10.134

    authors： Nakadate Y,Kodera Y,Kitamura Y,Tachibana T,Tamura T,Koizumi F

    更新日期：2013-11-29 00:00:00

  • Changes in autophagy proteins in a rat model of controlled cortical impact induced brain injury.

    abstract：:Autophagy has been implicated in several neurodegenerative diseases and recently its role in acute brain injury has received increased interest. In our study, we investigated the profiles of autophagy-linked proteins (MAP-LC3 (Atg8), beclin-1 (Atg6) and the beclin-1-binding protein, bcl-2, following controlled cortica...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.05.031

    authors： Sadasivan S,Dunn WA Jr,Hayes RL,Wang KK

    更新日期：2008-09-05 00:00:00

  • ESR spin trapping investigation on peroxynitrite decomposition: no evidence for hydroxyl radical production.

    abstract：:The decomposition of peroxynitrite in the presence of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) generated 5,5-dimethylpyrrolidone-(2)-oxy-(1) (DMPOX) without formation of DMPO/OH. Formate enhanced the peroxynitrite decomposition but did not generate any detectable amount of formate-derived free radicals. Glutathione, cy...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2357

    authors： Shi X,Lenhart A,Mao Y

    更新日期：1994-09-30 00:00:00

  • POU-domain genes in planarian Dugesia japonica: the structure and expression.

    abstract：:In fresh water planarian Dugesia japonica, two genes encoding POU-domain proteins, DjPOU1 and DjPOU2, have been identified by polymerase chain reaction (PCR). Their POU-domains are strikingly similar to class III POU-domains in mammals, frog, insects and nematode. The full-length cDNA of DjPOU1 has been cloned and seq...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1571

    authors： Orii H,Agata K,Watanabe K

    更新日期：1993-05-14 00:00:00

  • Interaction of casein kinase II with ribosomal protein L22 of Drosophila melanogaster.

    abstract：:The ubiquitous eukaryotic protein kinase CKII (casein kinase II) has been found to interact with a number of cellular proteins, either through the catalytic subunit or the regulatory subunit. Using the yeast two-hybrid screening method, we found that the catalytic subunit of Drosophila melanogaster CKII (DmCKII) inter...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02396-3

    authors： Zhao W,Bidwai AP,Glover CV

    更新日期：2002-10-18 00:00:00

  • ALKBH8 promotes bladder cancer growth and progression through regulating the expression of survivin.

    abstract：:Human AlkB homolog 8 (ALKBH8) is highly expressed in high-grade, superficially and deeply invasive bladder cancer. Moreover, ALKBH8 knockdown induces apoptosis in bladder cancer cells. However, the underlying anti-apoptotic mechanism of ALKBH8 in bladder cancer cells has thus far remained unclear. Moreover, there is n...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.06.084

    authors： Ohshio I,Kawakami R,Tsukada Y,Nakajima K,Kitae K,Shimanoe T,Saigo Y,Hase H,Ueda Y,Jingushi K,Tsujikawa K

    更新日期：2016-08-26 00:00:00