ESR spin trapping investigation on peroxynitrite decomposition: no evidence for hydroxyl radical production.

abstract：:An inhibitor against the egg beta-D-N-acetylglucosaminidase (GlcNAcase) was purified from the vitelline coat of eggs of the ascidian, Halocynthia roretzi. The purified inhibitor gave a single band with an apparent molecular mass of 70 kDa on SDS-PAGE under non-reducing conditions. The GlcNAcase inhibitor potently inhi...

journal_title：Biochemical and biophysical research communications

authors： Matsuura K,Sawada H,Yokosawa H

更新日期：1995-08-04 00:00:00

abstract：:We have cloned cDNAs encoding endothelial nitric oxide synthase (ecNOS) from a human fetal liver cDNA library. Overproduction of ecNOS in a baculovirus/insect cell expression system in conventional medium yielded a large amount of ecNOS protein localized in particulate components, but ecNOS activity was low. This acti...

journal_title：Biochemical and biophysical research communications

authors： Seo HG,Fujii J,Soejima H,Niikawa N,Taniguchi N

更新日期：1995-03-08 00:00:00

abstract：:Microinjection of purified transcriptionally active ribonucleoprotein (RNP) complex of vesicular stomatitis virus in vero cells resulted in the production and release of virus. Compared to the release of virus by cells treated with RNP in the presence of DEAE-dextran, the microinjection technique was highly efficient....

journal_title：Biochemical and biophysical research communications

authors： Thornton GB,Kopchick JJ,Stacey DW,Banerjee AK

更新日期：1983-11-15 00:00:00

abstract：:The levels of the anti-aging protein α-Klotho, in its soluble form (s-Klotho), are depressed in the circulation of patients with type 1 diabetes (T1D) or type 2 diabetes (T2D). Gene transfer experiments have suggested a protective role for β-cell specific expression of α-Klotho in murine models of T1D and T1D, but the...

journal_title：Biochemical and biophysical research communications

authors： Prud'homme GJ,Glinka Y,Kurt M,Liu W,Wang Q

更新日期：2020-05-07 00:00:00

abstract：:Evidence exists that the adenosine receptor A(2A)R and the dopamine receptor D(2)R form constitutive heteromers in living cells. Mass spectrometry and pull-down data showed that an arginine-rich domain of the D(2)R third intracellular loop binds via electrostatic interactions to a specific motif of the A(2A)R C-termin...

journal_title：Biochemical and biophysical research communications

authors： Borroto-Escuela DO,Marcellino D,Narvaez M,Flajolet M,Heintz N,Agnati L,Ciruela F,Fuxe K

更新日期：2010-03-26 00:00:00

abstract：:A wealth of evidence has shown that microglia-associated neuro-inflammation is involved in the secondary brain injury contributed to the poor outcome after traumatic brain injury (TBI). In vitro studies were reported that activation of metabotropic glutamate receptor 5 (mGluR5) could inhibit the microglia-associated i...

journal_title：Biochemical and biophysical research communications

authors： Wang JW,Wang HD,Cong ZX,Zhang XS,Zhou XM,Zhang DD

更新日期：2013-01-18 00:00:00

abstract：:Oxygen resistant N2-fixing Escherichia coli, which can grow at a high oxygen concentration in a nitrogen-free medium, were produced by several times of cultivation under a condition of 0.03% oxygen. Six isolated resistant strains could grow at an oxygen concentration ten times that of the parent strain. The nif-genes ...

journal_title：Biochemical and biophysical research communications

authors： Iwahashi H,Someya J

更新日期：1990-04-16 00:00:00

abstract：:It has been demonstrated that joint cultivation of human K-562 cells and mouse L-929 cells results in triggering apoptosis in L-929 cells and possibly also in K-562 cells. Analysis of proteins recovered from the culture medium has revealed the presence of three cytotoxic factors including TNF-alpha. This is the first ...

journal_title：Biochemical and biophysical research communications

authors： Gnuchev NV,Sashchenko LP,Iarovaia OV,Dukhanina E,Lukianova T,Razin SV

更新日期：1997-05-29 00:00:00

abstract：:Complete nucleotide sequence of the genes for subunits of the H+ ATPase of E.coli has been determined and several hybrid plasmids carrying various portions of these genes have been constructed. Genetic complementation and recombination tests of about forty mutants of E.coli defective in the ATPase were performed using...

journal_title：Biochemical and biophysical research communications

authors： Noumi T,Kanazawa H

更新日期：1983-02-28 00:00:00

abstract：:Polygonatum cyrtonema lectin (PCL), a mannose/sialic acid-binding plant lectin, has recently drawn a rising attention for cancer biologists because PCL bears remarkable anti-tumor activities and thus inducing programmed cell death (PCD) including apoptosis and autophagy in cancer cells. In this review, we focus on exp...

journal_title：Biochemical and biophysical research communications

authors： Wang SY,Yu QJ,Bao JK,Liu B

更新日期：2011-03-25 00:00:00

abstract：:Acidic fibroblast growth factors (FGF1s) are heparin binding proteins that regulate a wide array of key cellular processes and are also candidates for promising biomedical applications. FGF1-based therapeutic applications are currently limited due to their inherent thermal instability and susceptibility to proteases. ...

journal_title：Biochemical and biophysical research communications

authors： Kerr R,Agrawal S,Maity S,Koppolu B,Jayanthi S,Suresh Kumar G,Gundampati RK,McNabb DS,Zaharoff DA,Kumar TKS

更新日期：2019-10-15 00:00:00

abstract：:The self-induced endogenous elicitor isolated from potato having molecular weight 9200 was degraded with trifluoroacetic acid to determine the sugar composition. The requisite composition of the elicitor was estimated to be a 2:3:4:7 mixture of galactose, rhamnose, arabinose, and galacturonic acid. Furthermore, the st...

journal_title：Biochemical and biophysical research communications

authors： Monden T,Nakamura H,Murai A

更新日期：1995-10-13 00:00:00

abstract：:Co3O4 nanoparticles (NPs) are one kind of the important nanomaterials that have the application potential in catalyst, electrochromic devices, sensors, etc. However, their biological effect remains to be detailed. In this study, we investigated the effect of the as-synthesized Co3O4 NPs (15-30 nm) on the growth of mam...

journal_title：Biochemical and biophysical research communications

authors： Wang H,Ren T,Zhu N,Yu Q,Li M

更新日期：2018-11-02 00:00:00

abstract：:Here we investigate the effects of the dihydropyridine-type antagonists of calcium channels nitrendipine, nimodipine, nisoldipine and the calcium channel agonist BAY K 8644 on the induction of nitric oxide synthase (NOS) by bacterial endotoxin (lipopolysaccharide; LPS) in J774.2 macrophages cultured in vitro. Pretreat...

journal_title：Biochemical and biophysical research communications

authors： Szabó C,Thiemermann C,Vane JR

更新日期：1993-10-29 00:00:00

abstract：:Neuroblastoma LAN-5 cells exposed to retinoic acid cease to multiply and extend neurite outgrowths acquiring a neuronal phenotype. We now report that protein kinase C-theta; (PKC-theta;) isozyme is involved in this differentiation process due to the following findings: (i) PKC-theta; is expressed by LAN-5 cells as a n...

journal_title：Biochemical and biophysical research communications

authors： Sparatore B,Patrone M,Passalacqua M,Pedrazzi M,Pontremoli S,Melloni E

更新日期：2000-12-20 00:00:00

abstract：:Glucagon-like peptide-1 (GLP-1) receptor agonists have been used to treat type 2 diabetic patients and shown to reduce food intake and body weight. The anorexigenic effects of GLP-1 and GLP-1 receptor agonists are thought to be mediated primarily via the hypothalamic paraventricular nucleus (PVN). GLP-1, an intestinal...

journal_title：Biochemical and biophysical research communications

authors： Katsurada K,Maejima Y,Nakata M,Kodaira M,Suyama S,Iwasaki Y,Kario K,Yada T

更新日期：2014-08-22 00:00:00

abstract：:Novel conformation-specific antibodies were raised against a cyclic chimeric dodecapeptidyl multiple antigen peptide (cCD-MAP) constructed with a spacer-armed Gly-Asp dipeptide and two pentapeptides (S(169)-Q(170)-K(171)-E(172)-G(173) of CCR5 and E(179)-A(180)-D(181)-D(182)-R(183) of CXCR4) which are components of the...

journal_title：Biochemical and biophysical research communications

authors： Misumi S,Takamune N,Ido Y,Hayashi S,Endo M,Mukai R,Tachibana K,Umeda M,Shoji S

更新日期：2001-08-03 00:00:00

abstract：:Transgenic mice expressing the rhodopsin mutant Pro347Ser (Serine 6) display retinal degeneration through apoptosis that is characteristic of the disease retinitis pigmentosa. By 5 weeks after birth, these mice have lost approximately 35% of their photoreceptor cells. Retinas from these mice showed higher levels of cA...

journal_title：Biochemical and biophysical research communications

authors： Weiss ER,Hao Y,Dickerson CD,Osawa S,Shi W,Zhang L,Wong F

更新日期：1995-11-22 00:00:00

abstract：:We examined the contribution of apoptosis- and oxidative stress-associated genes to apoptosis induction in trophoblast cells of human fetal membrane tissues undergoing apoptosis during in vitro incubation. RT-PCR analyses demonstrated an increased level of HO-1, Mn-SOD, Cox-2, iNOS, TNFalpha, TNFR1, IL-1beta, IL-6, Ba...

journal_title：Biochemical and biophysical research communications

authors： Yuan B,Ohyama K,Bessho T,Toyoda H

更新日期：2006-03-17 00:00:00

abstract：:Accumulation of misfolded proteins within the lumen of the mammalian endoplasmic reticulum (ER) activates the unfolded protein response (UPR). ATF6 and Ire1p are ER-associated proteins that control UPR-specific transcription systems in mammals; UPR signaling involves cleavage of ATF6 and splicing of XBP1 mRNA initiate...

journal_title：Biochemical and biophysical research communications

authors： Shang J,Lehrman MA

更新日期：2004-04-30 00:00:00

abstract：:Blockading the negative-regulatory CTLA-4 receptor has emerged as a powerful strategy with clinical potential to enhance T-cell responses. Some experimental tumors, for example, are rejected when anti-CTLA-4 antibodies are administered in vivo. The concise target cells and downstream events, however, remain to be defi...

journal_title：Biochemical and biophysical research communications

authors： Cepero E,Hnatyszyn HJ,Kraus G,Lichtenheld MG

更新日期：1998-06-29 00:00:00

abstract：:Rheumatoid arthritis (RA) is one of the most common autoimmune diseases, but its autoimmune mechanisms are not clearly understood. Recently, anti-citrullinated peptide antibodies have been specifically observed in sera of RA patients. Furthermore, we identified RA-susceptible variant in a gene encoding citrullinating ...

journal_title：Biochemical and biophysical research communications

authors： Suzuki A,Yamada R,Ohtake-Yamanaka M,Okazaki Y,Sawada T,Yamamoto K

更新日期：2005-07-29 00:00:00

abstract：:The sequence of tryptic and chymotryptic peptides from cytosolic and mitochondrial rabbit liver serine hydroxymethyltransferase are compared to the proposed sequence of a protein coded for by the glyA gene of Escherichia coli. The E. coli glyA gene is believed to code for serine hydroxymethyltransferase. Extensive seq...

journal_title：Biochemical and biophysical research communications

authors： Barra D,Martini F,Angelaccio S,Bossa F,Gavilanes F,Peterson D,Bullis B,Schirch L

更新日期：1983-11-15 00:00:00

abstract：:Phosphoinositide phospholipase C (PLC) was extracted from the synaptic membrane fraction of rat brain by 1% sodium deoxycholate. The molecular weight and sedimentation coefficient of the membrane PLC were about 160,000 and 6.7 as estimated by gel filtration and sucrose density gradient centrifugation, respectively. Th...

journal_title：Biochemical and biophysical research communications

authors： Kozawa O,Hoshijima M,Tanimoto T,Ohmori T,Takai Y

更新日期：1987-05-29 00:00:00

abstract：:A cDNA for tetrameric carbonyl reductase was cloned from a pig lung cDNA library. The cDNA had an open reading frame encoding a protein of 244 amino acid residues with a predicted monomer molecular weight of 25,985. The authenticity of the cDNA was confirmed by a perfect match of 80 amino acids determined from sequenc...

journal_title：Biochemical and biophysical research communications

authors： Nakanishi M,Deyashiki Y,Nakayama T,Sato K,Hara A

更新日期：1993-08-16 00:00:00

abstract：:Chitin oligosaccharides elicited chitinase activity in melon plants. Hexamer to nonamer were the most efficient elicitors: hexamer for maximal stimulation of colorimetrically assessed chitinase activity, heptamer for maximal stimulation of radiochemically assessed chitinase activity. Chitinase elicitation was a rapid ...

journal_title：Biochemical and biophysical research communications

authors： Roby D,Gadelle A,Toppan A

更新日期：1987-03-30 00:00:00

abstract：:To analyze the mechanisms of mRNA export from the nucleus to the cytoplasm, we have isolated eleven mutants, ptr [poly(A)(+) RNA transport] 1 to 11, which accumulate poly(A)(+) RNA in the nucleus at a nonpermissive temperature in Schizosaccharomyces pombe. Of those, the ptr5-1 mutant shows dots- or a ring-like accumul...

journal_title：Biochemical and biophysical research communications

authors： Watanabe N,Ikeda T,Mizuki F,Tani T

更新日期：2012-02-03 00:00:00

abstract：:The potential association of CD2AP with the adherens junction protein E-cadherin, co-localization with the actin cytoskeleton, and involvement in cell migration was investigated in cultured rat gastric mucosal cells. In stationary cells, CD2AP was localized perinuclearly while E-cadherin was expressed along cell-cell ...

journal_title：Biochemical and biophysical research communications

authors： Mustonen H,Lepistö A,Lehtonen S,Lehtonen E,Puolakkainen P,Kivilaakso E

更新日期：2005-07-01 00:00:00

abstract：:Partial 3'-end nucleotide sequence of the pkI gene (GenBank accession No. AF019143) from Photobacterium leiognathi ATCC 25521 has been determined, and the encoded pyruvate kinase I is deduced. Pyruvate kinase I is the key enzyme of glycolysis, which converts phosphoenol pyruvate to pyruvate. Alignment and comparison o...

journal_title：Biochemical and biophysical research communications

authors： Lin JW,Lu HC,Chen HY,Weng SF

更新日期：1997-10-09 00:00:00

abstract：:TAZ (transcriptional co-activator with PDZ binding motif) is a transcriptional modulator of mesenchymal stem cell differentiation. We have found that TAZ was expressed in postconfluent 3T3-L1 preadipocytes and downregulated during differentiation. Downregulation of TAZ was specifically mediated by dexamethasone (DEX),...

journal_title：Biochemical and biophysical research communications

authors： He Q,Huang HY,Zhang YY,Li X,Qian SW,Tang QQ

更新日期：2012-03-16 00:00:00