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Substitution of the ISP alpha subunit of biphenyl dioxygenase from Pseudomonas results in a modification of the enzyme activity.

Abstract：

:We have constructed a hybrid multicomponent dioxygenase gene cluster in which the bphA1 gene, coding for ISP alpha subunit of biphenyl dioxygenase from Pseudomonas pseudoalcaligenes KF707, has been replaced by the bedC1 gene encoding the corresponding subunit of benzene dioxygenase from P. putida ML2. Escherichia coli cells containing the chimeric dioxygenase acquired the novel capability of producing indigo from indole. Furthermore, when compared to biphenyl dioxygenase, the hybrid dioxygenase enzyme was half as active towards benzene but exhibited only 4% activity when biphenyl was the substrate. The results implicate the ISP alpha subunit to be involved in substrate specificity and activity of the dioxygenase enzyme.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Tan HM,Cheong CM

doi

10.1006/bbrc.1994.2546

subject

Has Abstract

pub_date

1994-10-28 00:00:00

pages

912-7

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(84)72546-0

journal_volume

204

pub_type

杂志文章

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