Early and selective induction of apoptosis in human leukemic cells by the alkyl-lysophospholipid ET-18-OCH3.

abstract：:The conditions used in some immunoblot procedures can fail to detect calmodulin, S100 proteins, and other proteins with similar physical properties. We describe here some of the basis of this difficulty, and provide an immunoblot protocol that allows the rapid and reproducible detection of calmodulin and S100 beta in ...

journal_title：Biochemical and biophysical research communications

authors： Van Eldik LJ,Wolchok SR

更新日期：1984-11-14 00:00:00

abstract：:Transfer of the gene for L-gulono-gamma-lactone oxidase, the missing enzyme in L-ascorbic acid biosynthesis in scurvy-prone animals, into medaka (Oryzias latipes) was successfully done. The expression plasmid pSVL-GLO, carrying rat liver L-gulono-gamma-lactone oxidase cDNA, was microinjected into the cytoplasm of fert...

journal_title：Biochemical and biophysical research communications

authors： Toyohara H,Nakata T,Touhata K,Hashimoto H,Kinoshita M,Sakaguchi M,Nishikimi M,Yagi K,Wakamatsu Y,Ozato K

更新日期：1996-06-25 00:00:00

abstract：:The transcription factor hypoxia-inducible factor-1 (HIF-1) functions as a master regulator of hypoxic response by inducing the transcription of various genes responsible for cellular adaptation to hypoxia. In this study, we investigated the effects of protein inhibitor of activated STAT3 (PIAS3), a small ubiquitin-re...

journal_title：Biochemical and biophysical research communications

authors： Nakagawa K,Kohara T,Uehata Y,Miyakawa Y,Sato-Ueshima M,Okubo N,Asaka M,Takeda H,Kobayashi M

更新日期：2016-01-15 00:00:00

abstract：:Notch signaling is essential for cell-fate specification in metazoans, and dysregulation of the pathway leads to a variety of human diseases including heart and vascular defects as well as cancer. Glycosylation of the Notch extracellular domain has emerged as an elegant means for regulating Notch activity, especially ...

journal_title：Biochemical and biophysical research communications

authors： Takeuchi H,Haltiwanger RS

更新日期：2014-10-17 00:00:00

abstract：:The platelet glycoprotein (GP) Ib/IX/V is a hetero-oligomeric receptor complex for von Willebrand factor (vWF) and mediates platelet adhesion and aggregation under high shear stress conditions. It is composed of alpha and beta chain of GP Ib, GP IX, AND and GP V. To establish transgenic mice carrying human GP Ib alpha...

journal_title：Biochemical and biophysical research communications

authors： Kitaguchi T,Murata M,Kuramochi T,Kobayashi K,Ito M,Ueyama Y,Nomura T,Hikichi K,Miyakawa Y,Handa M,Hiraoka Y,Aiso S,Ikeda Y

更新日期：1996-03-18 00:00:00

abstract：OBJECTIVE:Chondrocytes express many kinds of hormone receptors. The function of Follicle stimulating hormone (FSH) in the ovary is mediated by FSH receptor (FSHR). FSH receptor (FSHR) is found in many non-ovarian tissues, however it has been unclear if chondrocytes express FSHR. The purpose of this study is to determin...

journal_title：Biochemical and biophysical research communications

authors： Kong D,Guan Q,Li G,Xin W,Qi X,Guo Y,Zhao J,Xu J,Sun S,Gao L

更新日期：2018-01-01 00:00:00

abstract：:The rat Bone/Liver/kidney/Placenta Alkaline Phosphatase (ALP) is transcribed from two alternative promoters spaced over 25 kb apart, resulting in two variant transcripts that are identical in their coding sequence. We investigated the steady-state levels of the two variant transcripts in various rat tissues and cell l...

journal_title：Biochemical and biophysical research communications

authors： Zernik J,Kream B,Twarog K

更新日期：1991-05-15 00:00:00

abstract：:The breakdown of blood clots requires the fibrinolytic action of the serine proteinase plasmin, a two-chain polypeptide derived posttranslationally from its precursor zymogen, plasminogen. While investigating plasminogen gene expression in human extrahepatic tissues, a cDNA sequence was obtained which closely resemble...

journal_title：Biochemical and biophysical research communications

authors： Weissbach L,Treadwell BV

更新日期：1992-07-31 00:00:00

abstract：:The translation terminator Sup35p assembles into self-replicating fibrillar aggregates that are responsible for the [PSI+] prion state. The Q/N-rich N-terminal domain together with the highly charged middle-domain (NM domain) drive the assembly of Sup35p into amyloid fibrils in vitro. NM domains are highly divergent a...

journal_title：Biochemical and biophysical research communications

authors： Kabani M,Melki R

更新日期：2020-08-27 00:00:00

abstract：:Neuromedin B and neuromedin C are the novel mammalian bombesin-like peptides isolated from porcine spinal cord. We have developed highly specific and sensitive radioimmunoassays for neuromedin B and neuromedin C, and determined their regional distribution in rat central nervous system. Prior to measurements of the tis...

journal_title：Biochemical and biophysical research communications

authors： Minamino N,Kangawa K,Matsuo H

更新日期：1984-11-14 00:00:00

abstract：:The pancreatic islets of Langerhans produce several peptide hormones, predominantly the metabolically active hormones insulin and glucagon, which are critical for maintaining normal fuel homeostasis. Some evidence exists that pancreatic endocrine cells turn over at a slow rate and can regenerate in certain conditions....

journal_title：Biochemical and biophysical research communications

authors： Hunziker E,Stein M

更新日期：2000-04-29 00:00:00

abstract：:Long repeated stretches of d(CCG) and tri-nucleotide are crucial mutations that cause hereditary forms of mental retardation (fragile X-syndrome). Moreover, the alternating (CG) di-nucleotide is one of the candidates for Z-DNA conformation. Solution NMR structure of d(CCGCGG)(2) has been solved and is discussed. The d...

journal_title：Biochemical and biophysical research communications

authors： Monleón D,Esteve V,Celda B

更新日期：2003-03-28 00:00:00

abstract：:Anandamide (arachidonylethanolamide) has been identified as a brain constituent that selectively binds to the cannabinoid receptor and possesses cannabimimetic activity. Cytochromes P450 catalyze the oxidation of arachidonic acid to several metabolites possessing very potent pharmacological activity. We examined wheth...

journal_title：Biochemical and biophysical research communications

authors： Bornheim LM,Kim KY,Chen B,Correia MA

更新日期：1993-12-15 00:00:00

abstract：:The aim of this study was to investigate hormonal factors responsible for the huge increase in PRL receptors on the day of estrus in the rat mammary gland. For this purpose, ovariectomized rats were primed with E2 so as to reach a physiological serum concentration of E2 (21.5 +/- 1.2 pg/ml) and high PRL serum values (...

journal_title：Biochemical and biophysical research communications

authors： Guillaumot P,Sabbagh I,Bertrand J,Cohen H

更新日期：1986-03-28 00:00:00

abstract：:Interleukin (IL)-33 is a novel member of the IL-1 family. IL-33 is primarily synthesized as a 30-kDa precursor (pro-IL-33). Pro-IL-33 is cleaved by caspase-1 into an 18-kDa mature form (mature IL-33) in vitro. Recombinant mature IL-33 has been known to induce T-helper type-2 (Th2)-associated cytokines and inflammatory...

journal_title：Biochemical and biophysical research communications

authors： Hayakawa M,Hayakawa H,Matsuyama Y,Tamemoto H,Okazaki H,Tominaga S

更新日期：2009-09-11 00:00:00

abstract：:An in organello footprinting approach has been used to probe a protein-DNA interaction of a nuclear coded 25 kDa protein, previously isolated in our laboratory, that binds "in vitro" a region within the ND2 gene, located upstream of the Ori-L. Footprinting studies with the purine-modifying reagent dimethyl sulfate and...

journal_title：Biochemical and biophysical research communications

authors： Cingolani G,Capaccio L,D'Elia D,Gadaleta G

更新日期：1997-02-24 00:00:00

abstract：:We have devised a method to evaluate the capacity of mammalian cell extracts to incise damaged DNA in vitro. The assay uses damaged-plasmid DNA as a substrate for nucleotide excision repair by cell extracts. During this process, enzymatic incision of the damaged DNA is followed by DNA resynthesis. Under our assay cond...

journal_title：Biochemical and biophysical research communications

authors： Calsou P,Salles B

更新日期：1994-07-29 00:00:00

abstract：:Flavonoids are a group of naturally occurring compounds used, e.g., in the treatment of vascular endothelial damage. They are known to be excellent scavengers of oxygen free radicals. Since the nitric oxide radical (.NO) probably plays a role in this pathology, the .NO scavenging capacity of the flavonoids was determi...

journal_title：Biochemical and biophysical research communications

authors： van Acker SA,Tromp MN,Haenen GR,van der Vijgh WJ,Bast A

更新日期：1995-09-25 00:00:00

abstract：:The retinoblastoma gene product (pRB) has been known to contain a sequence-nonspecific DNA binding activity. It is unknown whether pRB can recognize and bind to specific DNA sequences. We have recently identified a rat genomic DNA fragment, termed REC11, which can interact with rat Cdc37-related protein (RCdc37). In t...

journal_title：Biochemical and biophysical research communications

authors： Ozaki T,Sakiyama S

更新日期：1996-09-04 00:00:00

abstract：:Episomal vectors can allow efficient genetic modification of cells and have the potential advantage of avoiding chromosomal position of integration effects. Here we explore the use of an Epstein-Barr virus-based episomal vector with human embryonic stem (ES) cells, and find high initial transfection rates, but a rapid...

journal_title：Biochemical and biophysical research communications

authors： Kameda T,Smuga-Otto K,Thomson JA

更新日期：2006-11-03 00:00:00

abstract：:Due to their immune stimulating and apoptosis inducing properties, ligands of the TNF family attract increasing interest as therapeutic proteins. A general limitation of in vivo applications of recombinant soluble TNF ligands is their notoriously rapid clearance from circulation. To improve the serum half life of the ...

journal_title：Biochemical and biophysical research communications

authors： Müller N,Schneider B,Pfizenmaier K,Wajant H

更新日期：2010-06-11 00:00:00

abstract：:We present a novel and useful principle that can be applied to enzyme reactions with dilute substrates and enables to obtain the desired time-course curve from the reverse reaction when the latter can be measured more easily. Let us imagine an enzyme mixture that can catalyze the interconversion between substances A, ...

journal_title：Biochemical and biophysical research communications

authors： Kijima S,Kijima H

更新日期：1994-09-15 00:00:00

abstract：:Cadherins are cell adhesion molecules that modulate the epithelial phenotype and regulate tumor invasion. To identify the role of promoter methylation in regulating E-cadherin expression and in the "switching" of cadherins in oral squamous cell carcinoma (SCC), we studied 14 cell lines for cadherin expression. Immunob...

journal_title：Biochemical and biophysical research communications

authors： Chen Q,Lipkina G,Song Q,Kramer RH

更新日期：2004-03-19 00:00:00

abstract：:Neurotensin (NT), a gastrointestinal (GI) hormone, binds its receptor (NTR) to stimulate proliferation of normal and neoplastic GI tissues; the molecular mechanisms remain largely undefined. Mitogen-activated protein kinases (MAPKs) are a family of intracellular kinases that transmit mitogenic signals by translocating...

journal_title：Biochemical and biophysical research communications

authors： Ehlers RA,Zhang Y,Hellmich MR,Evers BM

更新日期：2000-03-24 00:00:00

abstract：:Many amplified-chromosome regions in human cancers have been identified, while there are only a limited number of reports for amplified genes in a single amplicon. We recently isolated four cDNA clones, A39, B47, C51, and CAB1, from 500 kilobases of yeast artificial chromosome DNA containing the c-ERBB-2 gene. B47 con...

journal_title：Biochemical and biophysical research communications

authors： Kishi T,Sasaki H,Akiyama N,Ishizuka T,Sakamoto H,Aizawa S,Sugimura T,Terada M

更新日期：1997-03-06 00:00:00

abstract：:Endothelin tightly bound to rabbit aortic strips and caused a prolonged vasoconstriction both in the presence and absence of extracellular Ca2+, although only partial constriction (20-30%) developed in the latter case, indicating that its action may not be limited to the opening of a calcium channel. The endothelin-in...

journal_title：Biochemical and biophysical research communications

authors： Sugiura M,Inagami T,Hare GM,Johns JA

更新日期：1989-01-16 00:00:00

abstract：:Western immunoblots of whole cell lysate and crude membrane extract of an in vitro selected sodium m-arsenite-resistant L. donovani strain revealed a 230-kDa protein identified by an anti-P-glycoprotein (Pgp) antibody. Immunofluorescence microscopy, using the same antibody, detected putative Pgp on resistant parasites...

journal_title：Biochemical and biophysical research communications

authors： Kaur J,Dey CS

更新日期：2000-05-19 00:00:00

abstract：:In mammals, behavioral and physiological processes display 24-h rhythms that are regulated by a circadian system. In the present study, we investigated the possibility that the expression of clock genes in peripheral leukocytes can be used to assess the circadian clock system. We found that Per1 and Per2 exhibit circa...

journal_title：Biochemical and biophysical research communications

authors： Fukuya H,Emoto N,Nonaka H,Yagita K,Okamura H,Yokoyama M

更新日期：2007-03-23 00:00:00

abstract：:miR-130b was significantly up-regulated in osteosarcoma (OS) cells. Naked cuticle homolog 2 (NKD2) inhibited tumor growth and metastasis in OS by suppressing Wnt signaling. We used three miRNA target analysis tools to identify potential targets of miR-130b, and found that NKD2 is a potential target of miR-130b. Based ...

journal_title：Biochemical and biophysical research communications

authors： Li Z,Li Y,Wang N,Yang L,Zhao W,Zeng X

更新日期：2016-03-18 00:00:00

abstract：:Glycogen storage disease type Ia (GSD-Ia) is caused by a deficiency in glucose-6-phosphatase-α (G6Pase-α or G6PC), a key enzyme in endogenous glucose production. This autosomal recessive disorder is characterized by impaired glucose homeostasis and long-term complications of hepatocellular adenoma/carcinoma (HCA/HCC)....

journal_title：Biochemical and biophysical research communications

authors： Cho JH,Kim GY,Mansfield BC,Chou JY

更新日期：2018-04-15 00:00:00