当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Expression of FSHR in chondrocytes and the effect of FSH on chondrocytes.

Expression of FSHR in chondrocytes and the effect of FSH on chondrocytes.

Abstract：

OBJECTIVE:Chondrocytes express many kinds of hormone receptors. The function of Follicle stimulating hormone (FSH) in the ovary is mediated by FSH receptor (FSHR). FSH receptor (FSHR) is found in many non-ovarian tissues, however it has been unclear if chondrocytes express FSHR. The purpose of this study is to determine it. METHODS:Mouse primary chondrocytes and human articular cartilage tissues were examined. The expression and sequence of FSHR mRNA by reverse transcription polymerase chain reaction (RT-PCR) and sequenced, respectively, and its protein expression was tested using western blotting and location was observed under immunofluorescence microscopy. Ovarian tissue was as a positive control. After FSH stimulated mouse chondrocytes, intracellular cAMP levels were assessed by ELISA, and gene expression relative to Mouse WNT Signaling Pathway was tested by RT2 Profiler PCR Arrays. RESULTS:FSHR was detected at the transcriptional level and confirmed to have the same sequence as that of ovary-derived mRNA of FSHR. FSHR proteins presented at the same line as the positive proteins of ovary, in mouse chondrocytes and human cartilage tissue, respectively. FSHR proteins were located at the cell membrane. Intracellular cAMP contents were significantly elevated up to 7-fold in mouse chondrocytes by forskolin (100 mM) (P < 0.001); however, different doses of FSH did not change the cAMP contents in mouse primary chondrocytes. RT2 Profiler PCR Arrays demonstrated that FSH could cause changes in gene expression among the 84 preordained genes, such as Fosl1, Rhou, and Dkk1, in mouse chondrocytes relative to the control. CONCLUSION:Mouse chondrocytes and human articular cartilage express functional FSHR. Moreover, FSH can act on chondrocytes and cause genetic changes.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kong D,Guan Q,Li G,Xin W,Qi X,Guo Y,Zhao J,Xu J,Sun S,Gao L

doi

10.1016/j.bbrc.2017.11.053

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

587-593

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(17)32231-3

journal_volume

495

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Identification of a human immunodeficiency virus-1 protease cleavage site within the 66,000 Dalton subunit of reverse transcriptase.

    abstract：:The human immunodeficiency virus-1 reverse transcriptase is a heterodimer of related 51 and 66 kDa subunits. The smaller subunit arises by viral protease-catalyzed cleavage of the carboxy-terminal domain of the 66 kDa species. Comparison of the amino acid composition analyses of the isolated 51 kDa and 66 kDa subunits...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91670-n

    authors： Graves MC,Meidel MC,Pan YC,Manneberg M,Lahm HW,Grüninger-Leitch F

    更新日期：1990-04-16 00:00:00

  • Role of excess lipoyl dehydrogenase in reconstituted alpha-ketoglutarate dehydrogenase complex of Escherichia coli.

    abstract：:The alpha-ketoglutarate dehydrogenase complex of Escherichia coli can bind up to 12 dimers of dihydrolipoyl dehydrogenase (E3) besides those already present. Maximal activity does not increase, however, when surplus E3 is present. This observation was previously interpreted to mean that the excess enzyme is inactive. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90999-x

    authors： Wagenknecht T,Francis N,DeRosier D

    更新日期：1986-03-28 00:00:00

  • Sulfoglycolipids bind to adhesive protein amphoterin (P30) in the nervous system.

    abstract：:HNK-1 antibody reactive carbohydrate epitope carried by glycolipids and glycoproteins has been shown to be involved in cell to cell interactions. It has been proposed that the HNK-1 reactive 3-sulfoglucuronyl carbohydrate epitope in glycolipids may interact with a cell surface receptor to promote the biological respon...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91787-q

    authors： Mohan PS,Laitinen J,Merenmies J,Rauvala H,Jungalwala FB

    更新日期：1992-01-31 00:00:00

  • Identification and distribution of endoplasmic reticulum iPLA2.

    abstract：:Our laboratory demonstrated that endoplasmic reticulum iPLA2 (ER-iPLA2) activity protects renal cells from oxidant-induced cell death and lipid peroxidation. The goals of this study were to determine the PLA2 isoform(s) responsible for ER-iPLA2 activity in different species and tissues. ER-iPLA2 activity was observed ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.12.016

    authors： Kinsey GR,Cummings BS,Beckett CS,Saavedra G,Zhang W,McHowat J,Schnellmann RG

    更新日期：2005-02-04 00:00:00

  • A radiobiological probe for simultaneous NMR spectroscopy and 192Ir gamma irradiation of Saccharomyces cerevisiae.

    abstract：:A novel nuclear magnetic resonance (NMR) spectroscopy probe was designed and constructed for the study of transient metabolic changes in cellular systems during exposure to ionizing radiation. The probe incorporated a bioreactor, a radiation source, and a radiofrequency detection circuit tunable between 100 and 300 MH...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6438

    authors： Magness JE,McFarland EW

    更新日期：1997-04-07 00:00:00

  • Overproduction of the Bradyrhizobium japonicum c-type cytochrome subunits of the cbb3 oxidase in Escherichia coli.

    abstract：:We report on a system to improve expression of mature c-type cytochromes in Escherichia coli. It is based on the use of plasmid pEC86 that expresses the E. coli cytochrome c maturation genes ccmABCDEFGH constitutively, whereby the production of both endogenous and foreign c-type cytochromes was increased substantially...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9549

    authors： Arslan E,Schulz H,Zufferey R,Künzler P,Thöny-Meyer L

    更新日期：1998-10-29 00:00:00

  • Role of N-terminal domain of streptokinase in protein transport.

    abstract：:Streptokinase (SK), an extracellular protein of several haemolytic strains of Streptococcus, is utilized as a potent thrombolytic agent for the treatment of various myocardial disorders. Functional properties of SK remain unchanged when the first 13 N-terminal amino acid (aa) residues are removed. At present, role of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1504

    authors： Pratap J,Kaur J,RajaMohan G,Singh D,Dikshit KL

    更新日期：1996-10-03 00:00:00

  • Unexpected relationships between bacteriophage lambda hypothetical proteins and bacteriophage T4 tail-fiber proteins.

    abstract：:Hypothetical lambda protein ORF314 shows significant homology with the carboxyl end of phage T4 tail-fiber protein gp37. Homology can also be demonstrated between hypothetical lambda protein ORF194 and a fragment of bacteriophage T4 protein gp38. This sequence homology is also reflected in the genomic sequences of the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(83)80043-6

    authors： George DG,Yeh LS,Barker WC

    更新日期：1983-09-30 00:00:00

  • Fcf1p and Fcf2p are novel nucleolar Saccharomyces cerevisiae proteins involved in pre-rRNA processing.

    abstract：:The uncharacterized Saccharomyces cerevisiae proteins Fcf1 and Fcf2, encoded by the ORFs YDR339c and YLR051c, respectively, were identified in a tandem affinity purification experiment of the known 40S factor Faf1p. Most of the proteins associated with TAP-Faf1p are trans-acting factors involved in pre-rRNA processing...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.05.140

    authors： Rempola B,Karkusiewicz I,Piekarska I,Rytka J

    更新日期：2006-07-28 00:00:00

  • Downregulation of ZMYND11 induced by miR-196a-5p promotes the progression and growth of GBM.

    abstract：:ZMYND11 (zinc finger MYND-type containing 11) has been widely regarded to be involved in a variety of cancers as a potential suppressor. However, the biological role and mechanism of ZMYND11 in glioblastoma multiform (GBM) remain unknown. In this study, we found that ZMYND11 expression was remarkably decreased in GBM ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.10.098

    authors： Yang JP,Yang JK,Li C,Cui ZQ,Liu HJ,Sun XF,Geng SM,Lu SK,Song J,Guo CY,Jiao BH

    更新日期：2017-12-16 00:00:00

  • CYLD, a deubiquitinase specific for lysine63-linked polyubiquitins, accumulates at the postsynaptic density in an activity-dependent manner.

    abstract：:Polyubiquitin chains on proteins flag them for distinct fates depending on the type of polyubiquitin linkage. While lysine48-linked polyubiquitination directs proteins to proteasomal degradation, lysine63-linked polyubiquitination promotes different protein trafficking and is involved in autophagy. Here we show that p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.10.131

    authors： Dosemeci A,Thein S,Yang Y,Reese TS,Tao-Cheng JH

    更新日期：2013-01-04 00:00:00

  • Identification of FOXE3 transcription factor as a potent oncogenic factor in triple-negative breast cancer.

    abstract：:Triple-negative breast cancer (TNBC) represents a unique subgroup of breast cancers (BCa) with potential to be highly proliferative and invasive. Patient with TNBC are prone to developing resistance to chemotherapy. Therefore, TNBC usually has a poor clinical outcome. The key factors driving these malignant features r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.12.034

    authors： Wang H,Yang T,Yuan Y,Sun X

    更新日期：2020-02-26 00:00:00

  • Opsonization of multiple drug resistant (MDR)-bacteria by antimicrobial peptide fused hepatitis B virus surface antigen (HBsAg) in vaccinated individuals.

    abstract：:Vaccination evoking immunity in susceptible individuals has become the most effective solution to combat infectious diseases. The surface antigen of hepatitis B virus (HBsAg) is a mandatory vaccine for children in China. Herein, we designed an antimicrobial protein consisting of an antimicrobial peptide Thanatin at th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.11.113

    authors： Fan X,Xu W,Gao W,Xiao H,Wu G

    更新日期：2021-01-01 00:00:00

  • Connective tissue growth factor induces cardiac hypertrophy through Akt signaling.

    abstract：:In the process of cardiac remodeling, connective tissue growth factor (CTGF/CCN2) is secreted from cardiac myocytes. Though CTGF is well known to promote fibroblast proliferation, its pathophysiological effects in cardiac myocytes remain to be elucidated. In this study, we examined the biological effects of CTGF in ra...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.03.100

    authors： Hayata N,Fujio Y,Yamamoto Y,Iwakura T,Obana M,Takai M,Mohri T,Nonen S,Maeda M,Azuma J

    更新日期：2008-05-30 00:00:00

  • Stabilization of C-terminal binding protein 2 by cellular inhibitor of apoptosis protein 1 via BIR domains without E3 ligase activity.

    abstract：:C-terminal binding protein 2 (CtBP2) is a transcriptional co-repressor that regulates many genes involved in normal cellular events. Because CtBP2 overexpression has been implicated in various human cancers, its protein levels must be precisely regulated. Previously, we reported that CtBP1 and CtBP1-mediated transcrip...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.05.098

    authors： Seo TW,Lee YT,Lee JS,Yoo SJ

    更新日期：2020-09-17 00:00:00

  • Dissection of Rab7 localization on Mycobacterium tuberculosis phagosome.

    abstract：:The late endosomal marker Rab7 has been long believed to be absent from the phagosome containing Mycobacterium tuberculosis (M.tb) in macrophage, but the detail kinetics remains elusive. Here, we found that Rab7 is transiently recruited to and subsequently released from M.tb phagosomes. For further understanding of th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.06.152

    authors： Seto S,Matsumoto S,Ohta I,Tsujimura K,Koide Y

    更新日期：2009-09-18 00:00:00

  • The oncoprotein HBXIP promotes migration of breast cancer cells via GCN5-mediated microtubule acetylation.

    abstract：:We have documented that the oncoprotein hepatitis B X-interacting protein (HBXIP) is able to promote migration of breast cancer cells. A subset of acetylated microtubules that accumulates in the cell leading edge is necessary for cell polarization and directional migration. In this study, we explored the hypothesis th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.02.036

    authors： Li L,Liu B,Zhang X,Ye L

    更新日期：2015-03-13 00:00:00

  • Identification of a complement receptor 1 peptide for inhibition of immune hemolysis.

    abstract：:Complement sensitization of red blood cells (RBCs) can cause life-threatening hemolytic anemias. We have previously shown that complement receptor 1 (CR1) derivatives specifically the N-terminal region with decay accelerating activity (DAA) for inactivation of a key enzyme in the complement cascade can reduce compleme...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.12.020

    authors： Yu J,Heck S,Debnath A,Yazdanbakhsh K

    更新日期：2007-02-09 00:00:00

  • Functional in vivo gene transfer into the myofibers of adult skeletal muscle.

    abstract：:The postmitotic nature and longevity of skeletal muscle fibers permit stable expression of any transfected gene. Direct in vivo injection of plasmid DNA, in both adult and regenerating muscles, is a safe, inexpensive, and easy approach. Here we present an optimized electroporation protocol based on the use of spatula ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.11.032

    authors： Donà M,Sandri M,Rossini K,Dell'Aica I,Podhorska-Okolow M,Carraro U

    更新日期：2003-12-26 00:00:00

  • An endogenous membrane-bound protein inhibits the phosphorylation of the L-type calcium channel by the cAMP-dependent protein kinase.

    abstract：:An endogenous protein inhibits the PKA-phosphorylation of the DHP-binding calcium channel complex in vitro. The inhibitory activity could be reduced by a treatment with detergents or dithiothreitol. Further purification separates the inhibitory activity from the dihydropyridine-binding calcium channel complex. Both ac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81589-x

    authors： Woscholski R,Marmé D

    更新日期：1992-08-14 00:00:00

  • A newly identified iron-binding protein in rat liver: purification and characterization.

    abstract：:A novel iron-binding protein from rat liver homogenates was purified 1,800-fold with a 5.7% yield, to apparent homogeneity. The molecular weight of the protein was estimated to be 16,000, by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The purified protein exhibited 0.43 mol of iron bi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81434-2

    authors： Furukawa T,Taketani S,Kohno H,Tokunaga R

    更新日期：1991-11-27 00:00:00

  • Selective destabilization of soluble amyloid beta oligomers by divalent metal ions.

    abstract：:Aggregation of the amyloid beta (Abeta) peptide yields both fibrillar precipitates and soluble oligomers, and is associated with Alzheimer's disease (AD). In vitro, Cu(2+) and Zn(2+) strongly bind Abeta and promote its precipitation. However, less is known about their interactions with the soluble oligomers, which are...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.04.056

    authors： Garai K,Sengupta P,Sahoo B,Maiti S

    更新日期：2006-06-23 00:00:00

  • Mutant proinsulin proteins associated with neonatal diabetes are retained in the endoplasmic reticulum and not efficiently secreted.

    abstract：:Mutations in the preproinsulin protein that affect processing of preproinsulin to proinsulin or lead to misfolding of proinsulin are associated with diabetes. We examined the subcellular localization and secretion of 13 neonatal diabetes-associated human proinsulin proteins (A24D, G32R, G32S, L35P, C43G, G47V, F48C, G...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.12.090

    authors： Park SY,Ye H,Steiner DF,Bell GI

    更新日期：2010-01-15 00:00:00

  • Sequence of a human keratin 13 specific cDNA encompassing coil 1B through the 3' end.

    abstract：:An expression library established in lambda gt11 with cDNA from squamous epithelium of the human upper digestive tract was screened with an antibody raised against keratin 13. A 1.2 kb fragment from the most strongly reacting plaque was sequenced and compared to known type I keratin sequences. The highest degree of ho...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)90847-4

    authors： Schulz P,Wachter E,Hochstrasser K,Wild AG,Mischke D

    更新日期：1989-08-15 00:00:00

  • Induction of hepatocyte growth by intraportal infusion of HGF into beagle dogs.

    abstract：:Hepatocyte growth factor (HGF), originally identified as a potent mitogen for mature parenchymal hepatocytes, is a hepatotrophic factor involved in liver regeneration and is even essential for development of the liver. We report here that human recombinant HGF at a very low concentration and given intraportaly stimula...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0347

    authors： Kobayashi Y,Hamanoue M,Ueno S,Aikou T,Tanabe G,Mitsue S,Matsumoto K,Nakamura T

    更新日期：1996-03-07 00:00:00

  • Thyroglobulin structure-function: the effect of iodination on the structure of human thyroglobulin.

    abstract：:Thyroglobulin of very low iodine content has been prepared from a single non-toxic human goitre. The initial iodine content of the protein (0.038%) has been increased to levels of 0.16% and 0.85% by in vitro treatment with thyroid peroxidase and the resulting proteins studied with respect to their intrinsic fluorescen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90724-1

    authors： Zabel KM,Rawitch AB

    更新日期：1984-05-31 00:00:00

  • Proteomic analysis reveals a protective role for DJ-1 during 6-hydroxydopamine-induced cell death.

    abstract：:Loss-of-function mutations in the DJ-1/PARK7 gene are responsible for early-onset autosomal-recessive Parkinson's disease. DJ-1 is implicated in the protection of neurons from oxidative stress by scavenging hydrogen peroxide and regulating the transcriptional activity of multiple pathways. Here, we attempted to identi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.04.063

    authors： Kim SJ,Park YJ,Oh YJ

    更新日期：2012-05-25 00:00:00

  • CK2 regulates in vitro the activity of the yeast cyclin-dependent kinase inhibitor Sic1.

    abstract：:We have previously demonstrated that the cyclin-dependent kinase inhibitor (Cki) Sic1 of Saccharomyces cerevisiae is phosphorylated in vitro by the CK2 kinase on Ser(201) residue. Moreover, we have collected evidence showing that Sic1 is functionally and structurally related to mammalian Cki p27(Kip1) and binds to the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.08.224

    authors： Barberis M,Pagano MA,Gioia LD,Marin O,Vanoni M,Pinna LA,Alberghina L

    更新日期：2005-11-04 00:00:00

  • Leukotriene B4 stimulates human monocyte-derived dendritic cell chemotaxis.

    abstract：:Although leukotriene B4 (LTB4) has been reported to stimulate monocytes and neutrophils, its role on dendritic cell (DC) activity has not been examined. Here, we investigated the expression of LTB4 receptor and the effect of LTB4 on human DC chemotaxis. We analyzed LTB4 receptors, BLT1 and BLT2, by using RT-PCR. DCs e...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.07.084

    authors： Shin EH,Lee HY,Bae YS

    更新日期：2006-09-22 00:00:00

  • Co-localization of a glucose transporter and the insulin receptor in microsomes of insulin-treated rat adipocytes.

    abstract：:Microsomal vesicles prepared from rat adipocytes were immuno-adsorbed to formaldehyde-fixed Staphylococcus aureus cells (Pansorbin) coated with anti-human-erythrocyte-glucose-transporter IgG. More than 75% of the glucose transporter detected was precipitated. The glucose transporter was about 10-fold enriched by the a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92261-4

    authors： Ezaki O,Bono N,Itakura H,Kasahara M

    更新日期：1989-03-31 00:00:00