Abstract:
:An expression library established in lambda gt11 with cDNA from squamous epithelium of the human upper digestive tract was screened with an antibody raised against keratin 13. A 1.2 kb fragment from the most strongly reacting plaque was sequenced and compared to known type I keratin sequences. The highest degree of homology was detected with the murine 47K type I keratin, which we consider to be the counterpart of human keratin 13. Tryptic peptides of keratin 13 were separated on a HPLC column and one peptide was sequenced. The amino acid sequence obtained supports the identity of the cDNA. An eight codon motif has been tandemly repeated in the C-domain of keratin 13. In spite of substantial divergence by point mutations and deletions, the remaining sequence homologies suggest that the C-domains of both the human keratin 13 and the orthologous murine protein have originated from a common ancestor.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Schulz P,Wachter E,Hochstrasser K,Wild AG,Mischke Ddoi
10.1016/0006-291x(89)90847-4subject
Has Abstractpub_date
1989-08-15 00:00:00pages
1522-7issue
3eissn
0006-291Xissn
1090-2104pii
0006-291X(89)90847-4journal_volume
162pub_type
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