Abstract:
:We report high resolution images of RecA-double stranded (ds) DNA complexes obtained by atomic force microscopy (AFM). When a carbon nanotube (CNT) tip was used, AFM images visualized the 10-nm pitch of RecA-dsDNA complexes and RecA filaments as three-dimensional surface topography without reconstruction analysis. The depth of the notch between two pitches was less than 1 nm. When adsorbed on a soft surface covered with proteins, naked DNA, RecA monomers, RecA hexamers, and short RecA filaments were all clearly resolved in one image. The high resolution images with a CNT tip provided valuable information on the initiation process of RecA-dsDNA complex formation.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Umemura K,Komatsu J,Uchihashi T,Choi N,Ikawa S,Nishinaka T,Shibata T,Nakayama Y,Katsura S,Mizuno A,Tokumoto H,Ishikawa M,Kuroda Rdoi
10.1006/bbrc.2001.4333subject
Has Abstractpub_date
2001-02-23 00:00:00pages
390-5issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(01)94333-5journal_volume
281pub_type
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