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Functional in vivo gene transfer into the myofibers of adult skeletal muscle.

Abstract：

:The postmitotic nature and longevity of skeletal muscle fibers permit stable expression of any transfected gene. Direct in vivo injection of plasmid DNA, in both adult and regenerating muscles, is a safe, inexpensive, and easy approach. Here we present an optimized electroporation protocol based on the use of spatula electrodes to transfer cDNA in vivo into the adult myofibers of an anatomically defined muscle, which could be functionally characterized. In our hands, about 80% of adult myofibers were transfected in vivo by different plasmids for GFP fusion proteins or for beta-galactosidase. The luciferase activity increased several orders of magnitude when compared to standard DNA delivery. In an anatomical defined muscle, the wide gene transfer was comparable to or better than that of retrovirus delivery, that recently has been shown to be prone to severe side-effects in human clinical studies. Furthermore, with our method the tissue damage was greatly decreased. Thus, the present work describes in vivo functional electrotransfer of genes in adult skeletal muscle fibers by a protocol that is of great potential for gene therapy, as well as for basic research.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Donà M,Sandri M,Rossini K,Dell'Aica I,Podhorska-Okolow M,Carraro U

doi

10.1016/j.bbrc.2003.11.032

subject

Has Abstract

pub_date

2003-12-26 00:00:00

pages

1132-8

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006291X03023805

journal_volume

312

pub_type

杂志文章

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