The serine protease HtrA2 cleaves UCH-L1 and inhibits its hydrolase activity: implication in the UCH-L1-mediated cell death.


:Ubiquitin (Ub) carboxyl-terminal hydrolase L1 (UCH-L1) has dual functions, such as hydrolase activity on the chemical bonds formed by the C-terminal Gly of Ub and dimerization-dependent ubiquitin ligase activity. Accumulating evidence suggests that dual activities of UCH-L1 were intimately associated with Parkinson's diseases (PD) and cancer. However, the molecular mechanism that regulates UCH-L1 enzymatic activity has not yet been fully elucidated. The serine protease high temperature requirement A2 (HtrA2), a PD-associated gene, is important in regulating cell survival as well as apoptosis. Using in vitro and in vivo cleavage assays, we have demonstrated that UCH-L1 is a natural substrate for the serine protease HtrA2 in the apoptotic pathway. Notably, we show that released, cytosolic HtrA2 decreases UCH-L1 protein level and its hydrolase activity through HtrA2-mediated cleavage of UCH-L1 under apoptotic conditions. These findings suggest that the HtrA2-mediated cleavage of UCH-L1 may play important roles in regulating the fine balance between cell growth and cell death.


Park DW,Nam MK,Rhim H




Has Abstract


2011-11-11 00:00:00














  • Imatinib mesylate elicits extracellular signal-related kinase (ERK) activation and enhances the survival of γ-irradiated epithelial cells.

    abstract::Imatinib mesylate, commercially known as Gleevec/Glivec, is the first targeted anticancer drug that inhibits activity of the tyrosine kinases, c-ABL, c-KIT, and PDGFR. A number of studies have shown that treatment with imatinib mesylate elicits extracellular signal-related kinase (ERK) activation, which, in turn, has ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lee J,Ko J,Kim HJ,Im H,Kim EJ,Yi JY

    更新日期:2018-12-02 00:00:00

  • Increased gene expression and production of murine endothelin receptors after birth.

    abstract::We developed the real-time PCR quantification of endothelin-A (ET-A) and endothelin-B (ET-B) receptor genes and present their relative expression levels in various adult tissues and during development in mouse using the 2(-Delta Delta C(T)) method. ET-A and ET-B receptors were detected in all tissues examined. Gene ex...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Adur J,Takizawa S,Quan J,Uchide T,Saida K

    更新日期:2003-06-06 00:00:00

  • Phorbol ester induces differential membrane-association of protein kinase C subspecies in human platelets.

    abstract::Human platelets contained proteins which cross-reacted with antisera specific for brain protein kinase C-alpha and -beta. When platelets were incubated with 12-O-tetradecanoylphorbol-13-acetate there was a rapid accumulation of protein kinase C-alpha in the particulate fraction associated with a loss of this subspecie...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fournier A,Hardy SJ,Clark KJ,Murray AW

    更新日期:1989-06-15 00:00:00

  • Nitric oxide toxicity in islet cells involves poly(ADP-ribose) polymerase activation and concomitant NAD+ depletion.

    abstract::Previous studies have shown that DNA strand breaks are an early consequence of nitric oxide toxicity in pancreatic islet cells. We show here that exposure of islet cells to chemical NO donors causes the formation of ADP-ribose polymers in cell nuclei, with concomitant depletion of intracellular NAD+. Islet cell lysis ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Radons J,Heller B,Bürkle A,Hartmann B,Rodriguez ML,Kröncke KD,Burkart V,Kolb H

    更新日期:1994-03-30 00:00:00

  • Preparation of an antibody recognizing both human and rodent MRP1.

    abstract::Based on the high level of identity among human, mouse, and rat MRP1 protein sequence, we produced a specific polyclonal antibody (MRP1-A23) against a synthetic polypeptide covering the C-terminus of the human protein. Western blot analysis showed a reactivity against human MRP1 similar to that obtained with the monoc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fernetti C,Pascolo L,Podda E,Gennaro R,Stebel M,Tiribelli C

    更新日期:2001-11-09 00:00:00

  • Characterization of a new calcium-binding protein abundant in amniotic fluid, CAAF2, which is produced by fetal epidermal keratinocytes during embryogenesis.

    abstract::By using a 45Ca2+ overlay technique, we found two calcium-binding proteins (CaBPs) abundant in bovine amniotic fluid, and named them CAAF1 (calcium-binding protein in amniotic fluid-1) and CAAF2. CAAF1 was identified as a novel S100 protein produced by fetal epidermal keratinocytes, squamous epithelial cells and polym...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hitomi J,Maruyama K,Kikuchi Y,Nagasaki K,Yamaguchi K

    更新日期:1996-11-21 00:00:00

  • Simultaneous detection of thiamine and its phosphate esters from microalgae by HPLC.

    abstract::We present an easy and sensitive method for measuring thiamine and its phosphate esters in small biological samples of microalgae (Amphidinium carterae Hulburt and Nitzschia microcephala Grun). The method consists of extraction of thiamine and its derivatives in acid solution, followed by liquid chromatography with fl...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pinto E,Pedersén M,Snoeijs P,Van Nieuwerburgh L,Colepicolo P

    更新日期:2002-02-22 00:00:00

  • Selective inactivation of MAO-B by benzyl-dimethyl-silyl-methanamines in vitro.

    abstract::The alpha-silyl amines benzyl-dimethyl-silyl-methanamine and the p-fluoro and p-chloro derivatives are potent time-dependent inhibitors of rat brain MAO-B. The inhibition exhibits saturation kinetics, takes place in the enzyme active-site and is irreversible. The most potent inhibitor in the series is 4-fluorobenzyl-d...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Danzin C,Collard JN,Marchal P,Schirlin D

    更新日期:1989-04-28 00:00:00

  • Sites on FIP-3 (NEMO/IKKgamma) essential for its phosphorylation and NF-kappaB modulating activity.

    abstract::FIP-3 (NEMO/IKKgamma) is an essential modulator of the activity of NF-kappaB by mechanisms that include alterations in the phosphorylation, ubiquination, and degradation of IkappaBalpha. The multiple protein-protein interactions of FIP-3 (NEMO/IKKgamma) in a high molecular weight IKK complex indicated that this protei...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tarassishin L,Horwitz MS

    更新日期:2001-07-13 00:00:00

  • Acetaldehyde-dependent oxidation of glutathione catalyzed by rat liver cytosol.

    abstract::We have identified a novel reaction in which acetaldehyde promotes rat hepatic cytosolic catalysis of O2 consumption coupled with glutathione oxidation without apparent release of activated forms of O2. Acetaldehyde is not consumed in the reaction. The reaction (O2 consumption or oxidized glutathione production) is sa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lyon I,Kaplowitz N

    更新日期:1985-06-28 00:00:00

  • Amantadine- and oseltamivir-resistant variants of influenza A viruses in Thailand.

    abstract::Amantadine and oseltamivir are used to treat influenza A virus infections; however, resistance to these drugs has been widely reported throughout the world. In this study, the frequency and genetic characteristics of the drug-resistant influenza A viruses that circulated in Thailand from 2006 to 2008 were investigated...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bai GR,Chittaganpitch M,Kanai Y,Li YG,Auwanit W,Ikuta K,Sawanpanyalert P

    更新日期:2009-12-18 00:00:00

  • Rundown of GH3 cell K+ conductance response to TRH following patch recording can be obviated with GH3 cell extract.

    abstract::GH3/B6 pituitary cells release prolactin (PRL) in response to thyrotropin releasing hormone (TRH). Electrophysiological assays of individual GH3 cells with sharp high-resistance microelectrodes have revealed complex effects of TRH on membrane excitability consisting of a transient hyperpolarization (1), which is thoug...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dufy B,MacDermott A,Barker JL

    更新日期:1986-05-29 00:00:00

  • Identification of FOXE3 transcription factor as a potent oncogenic factor in triple-negative breast cancer.

    abstract::Triple-negative breast cancer (TNBC) represents a unique subgroup of breast cancers (BCa) with potential to be highly proliferative and invasive. Patient with TNBC are prone to developing resistance to chemotherapy. Therefore, TNBC usually has a poor clinical outcome. The key factors driving these malignant features r...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang H,Yang T,Yuan Y,Sun X

    更新日期:2020-02-26 00:00:00

  • Soluble PTK7 inhibits tube formation, migration, and invasion of endothelial cells and angiogenesis.

    abstract::Human PTK7 is a defective receptor protein tyrosine kinase and its expression is upregulated in various cancers including colorectal carcinomas. To determine whether PTK7 functions in angiogenesis, we have expressed and purified the extracellular domain of PTK7 (soluble PTK7; sPTK7) as a decoy receptor to counteract t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Shin WS,Maeng YS,Jung JW,Min JK,Kwon YG,Lee ST

    更新日期:2008-07-11 00:00:00

  • Gene organization and 5'-flanking region sequence of conglutinin: a C-type mammalian lectin containing a collagen-like domain.

    abstract::Bovine conglutinin is a Ca(2+)-dependent serum lectin specific for N-acetylglucosamine. In the present study, we report the exon-intron organization and the sequence of 5'-regulatory region of the conglutinin gene. The coding region of conglutinin mRNA, which encoded the signal peptide and the sequence of the mature p...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kawasaki N,Itoh N,Kawasaki T

    更新日期:1994-01-28 00:00:00

  • Alterations in expression and structure of the DNA repair gene XRCC1.

    abstract::The repair-associated gene XRCC1 was previously cloned by complementing the hamster mutant EM9, which has a high rate of spontaneous SCE and hypersensitivity to DNA damaging agents. In analyzing XRCC1 gene expression, similar levels of steady-state mRNA were found in normal cells, Bloom's syndrome cells with altered S...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yoo H,Li L,Sacks PG,Thompson LH,Becker FF,Chan JY

    更新日期:1992-07-31 00:00:00

  • Isolation of rat adrenocortical mitochondria.

    abstract::This report describes a relatively simple and reliable method for isolating adrenocortical mitochondria from rats in good, reasonably pure yield. These organelles, which heretofore have been unobtainable in isolated form from small laboratory animals, are now readily accessible. A high degree of mitochondrial purity i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Solinas P,Fujioka H,Tandler B,Hoppel CL

    更新日期:2012-10-12 00:00:00

  • Inhibition of the acetyl lysine-binding pocket of bromodomain and extraterminal domain proteins interferes with adipogenesis.

    abstract::The bromodomain and extraterminal (BET) domain family proteins are epigenetic modulators involved in the reading of acetylated lysine residues. The first BET protein inhibitor to be identified, (+)-JQ1, a thienotriazolo-1, 4-diazapine, binds selectively to the acetyl lysine-binding pocket of BET proteins. We evaluated...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Goupille O,Penglong T,Kadri Z,Granger-Locatelli M,Fucharoen S,Maouche-Chrétien L,Prost S,Leboulch P,Chrétien S

    更新日期:2016-04-15 00:00:00

  • Knockdown of TIGAR by RNA interference induces apoptosis and autophagy in HepG2 hepatocellular carcinoma cells.

    abstract::Apoptosis and autophagy are crucial mechanisms regulating cell death, and the relationship between apoptosis and autophagy in the liver has yet to be thoroughly explored. TIGAR (TP53-induced glycolysis and apoptosis regulator), which is a p53-inducible gene, functions in the suppression of ROS (reactive oxygen species...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ye L,Zhao X,Lu J,Qian G,Zheng JC,Ge S

    更新日期:2013-07-26 00:00:00

  • Inhibitors of protein phosphatases 1 and 2A differentially prevent intrinsic and extrinsic apoptosis pathways.

    abstract::Inhibitors of serine/threonine protein phosphatases can inhibit apoptosis. We investigated which protein phosphatases are critical for this protection using calyculin A, okadaic acid, and tautomycin. All three phosphatase inhibitors prevented anisomycin-induced apoptosis in leukemia cell models. In vitro, calyculin A ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chatfield K,Eastman A

    更新日期:2004-10-29 00:00:00

  • Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane.

    abstract::The carboxyl terminus of the type 1 angiotensin II receptor (AT(1)) plays an important role in receptor phosphorylation, desensitization, and internalization. The yeast two-hybrid system was employed to isolate proteins associated with the carboxyl terminal region of the AT(1A) receptor. In the present study, we repor...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Guo DF,Chenier I,Tardif V,Orlov SN,Inagami T

    更新日期:2003-10-31 00:00:00

  • Synergism of vitamins A and C on fibrinolysis.

    abstract::A hitherto unknown synergism exerted by retinol (vitamin A) and L-ascorbic acid (vitamin C) was discovered using endothelial cells. Retinol stimulated the extracellular and intracellular activities of plasminogen activator up to approximately 8- and 4-fold from the control values, respectively. L-Ascorbic acid enhance...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Inada Y,Hagiwara H,Kojima S,Shimonaka M,Saito Y

    更新日期:1985-07-16 00:00:00

  • CutC is induced late during copper exposure and can modify intracellular copper content in Enterococcus faecalis.

    abstract::Copper is a micronutrient that is required for proper metabolic functioning of most prokaryotic and eukaryotic organisms. To sustain an adequate supply of copper, a cell requires molecular mechanisms that control the metal content to avoid copper toxicity. This toxicity comes primarily from the reactivity of copper, w...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Latorre M,Olivares F,Reyes-Jara A,López G,González M

    更新日期:2011-03-25 00:00:00

  • Expression profile analysis of colon cancer cells in response to sulindac or aspirin.

    abstract::Nonsteroidal anti-inflammatory drugs (NSAIDs) have a preventive effect against colorectal cancer. Although inhibition of cyclooxygenase-2 plays a crucial role in the suppression of tumors, precise mechanisms of their action remain to be disclosed. To identify genes involved in the growth-suppressive effect of NSAIDs, ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Iizaka M,Furukawa Y,Tsunoda T,Akashi H,Ogawa M,Nakamura Y

    更新日期:2002-03-29 00:00:00

  • Nucleotide triphosphatase activity of the N-terminal nucleotide-binding domains of the multidrug resistance proteins P-glycoprotein and MRP1.

    abstract::The multidrug resistance proteins P-glycoprotein (Pgp) and MRP1 are drug-efflux pumps. In this study, we compared the nucleotide triphosphatase activities of the isolated N-terminal nucleotide binding domains (NBD1) of Pgp and MRP1, and explored the potential role of the phosphorylation target domain of Pgp on the reg...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wilkes DM,Wang C,Aristimuño PC,Castro AF,Altenberg GA

    更新日期:2002-08-16 00:00:00

  • Dramatic suppression of colorectal cancer cell growth by the dual mTORC1 and mTORC2 inhibitor AZD-2014.

    abstract::Colorectal cancer is a major contributor of cancer-related mortality. The mammalian target or rapamycin (mTOR) signaling is frequently hyper-activated in colorectal cancers, promoting cancer progression and chemo-resistance. In the current study, we investigated the anti-colorectal cancer effect of a novel mTOR comple...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Huo HZ,Zhou ZY,Wang B,Qin J,Liu WY,Gu Y

    更新日期:2014-01-10 00:00:00

  • TFDP3 inhibits E2F1-induced, p53-mediated apoptosis.

    abstract::By dimerizing with E2F proteins, TFDP has profound influence on cellular E2F activities. While TFDP1 and 2 enhance the DNA binding and the transcriptional activity of E2F, the newly identified member of the DP family, TFDP3 primarily functions as a negative regulator. To further characterize the inhibitory property of...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tian C,Lv D,Qiao H,Zhang J,Yin YH,Qian XP,Wang YP,Zhang Y,Chen WF

    更新日期:2007-09-14 00:00:00

  • Delaying aging in Caenorhabditis elegans with protein aggregation inhibitors.

    abstract::Recent evidence suggests that during aging there is widespread accumulation of aggregated insoluble proteins, even in the absence of pathological conditions. Pharmacological manipulation of protein aggregation might be helpful to unveil the involvement of protein aggregates during aging, as well as to develop novel st...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Cuanalo-Contreras K,Park KW,Mukherjee A,Millán-Pérez Peña L,Soto C

    更新日期:2017-01-01 00:00:00

  • Altered protoxin activation by midgut enzymes from a Bacillus thuringiensis resistant strain of Plodia interpunctella.

    abstract::Processing of Bacillus thuringiensis protoxins to toxins by midgut proteinases from a strain of the Indianmeal moth, Plodia interpunctella (Hubner), resistant to B. thuringiensis subspecies entomocidus (HD-198) was slower than that by midgut proteinases from the susceptible parent strain or a strain resistant to B. th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Oppert B,Kramer KJ,Johnson DE,MacIntosh SC,McGaughey WH

    更新日期:1994-02-15 00:00:00

  • Hepatitis C NS3 protease: restoration of NS4A cofactor activity by N-biotinylation of mutated NS4A using synthetic peptides.

    abstract::The NS3 serine protase of Hepatitis C virus (HCV) requires NS4A protein as a cofactor for efficient cleavage at four sites in the nonstructural region. The cofactor activity has been mapped to the central hydrophobic region (aa 22-34) of this 54-amino-acid NS4A protein, and site-directed mutagenesis has identified alt...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Butkiewicz NJ,Yao N,Wright-Minogue J,Zhang R,Ramanathan L,Lau JY,Hong Z,Dasmahapatra B

    更新日期:2000-01-07 00:00:00